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Bacterial symbiont and salivary peptide evolution in the context of leech phylogeny.
Parasitology. 2011 Nov; 138(13):1815-27.P

Abstract

The evolutionary history of leeches is employed as a general framework for understanding more than merely the systematics of this charismatic group of annelid worms, and serves as a basis for understanding blood-feeding related correlates ranging from the specifics of gut-associated bacterial symbionts to salivary anticoagulant peptides. A variety of medicinal leech families were examined for intraluminal crop bacterial symbionts. Species of Aeromonas and Bacteroidetes were characterized with DNA gyrase B and 16S rDNA. Bacteroidetes isolates were found to be much more phylogenetically diverse and suggested stronger evidence of phylogenetic correlation than the gammaproteobacteria. Patterns that look like co-speciation with limited taxon sampling do not in the full context of phylogeny. Bioactive compounds that are expressed as gene products, like those in leech salivary glands, have 'passed the test' of evolutionary selection. We produced and bioinformatically mined salivary gland EST libraries across medicinal leech lineages to experimentally and statistically evaluate whether evolutionary selection on peptides can identify structure-function activities of known therapeutically relevant bioactive compounds like antithrombin, hirudin and antistasin. The combined information content of a well corroborated leech phylogeny and broad taxonomic coverage of expressed proteins leads to a rich understanding of evolution and function in leech history.

Authors+Show Affiliations

Sackler Institute of Comparative Genomics & Division of Invertebrate Zoology, American Museum of Natural History, New York, NY, USA. siddall@amnh.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21729354

Citation

Siddall, Mark E., et al. "Bacterial Symbiont and Salivary Peptide Evolution in the Context of Leech Phylogeny." Parasitology, vol. 138, no. 13, 2011, pp. 1815-27.
Siddall ME, Min GS, Fontanella FM, et al. Bacterial symbiont and salivary peptide evolution in the context of leech phylogeny. Parasitology. 2011;138(13):1815-27.
Siddall, M. E., Min, G. S., Fontanella, F. M., Phillips, A. J., & Watson, S. C. (2011). Bacterial symbiont and salivary peptide evolution in the context of leech phylogeny. Parasitology, 138(13), 1815-27. https://doi.org/10.1017/S0031182011000539
Siddall ME, et al. Bacterial Symbiont and Salivary Peptide Evolution in the Context of Leech Phylogeny. Parasitology. 2011;138(13):1815-27. PubMed PMID: 21729354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bacterial symbiont and salivary peptide evolution in the context of leech phylogeny. AU - Siddall,Mark E, AU - Min,Gi-Sik, AU - Fontanella,Frank M, AU - Phillips,Anna J, AU - Watson,Sara C, Y1 - 2011/05/10/ PY - 2011/7/7/entrez PY - 2011/7/7/pubmed PY - 2012/1/31/medline SP - 1815 EP - 27 JF - Parasitology JO - Parasitology VL - 138 IS - 13 N2 - The evolutionary history of leeches is employed as a general framework for understanding more than merely the systematics of this charismatic group of annelid worms, and serves as a basis for understanding blood-feeding related correlates ranging from the specifics of gut-associated bacterial symbionts to salivary anticoagulant peptides. A variety of medicinal leech families were examined for intraluminal crop bacterial symbionts. Species of Aeromonas and Bacteroidetes were characterized with DNA gyrase B and 16S rDNA. Bacteroidetes isolates were found to be much more phylogenetically diverse and suggested stronger evidence of phylogenetic correlation than the gammaproteobacteria. Patterns that look like co-speciation with limited taxon sampling do not in the full context of phylogeny. Bioactive compounds that are expressed as gene products, like those in leech salivary glands, have 'passed the test' of evolutionary selection. We produced and bioinformatically mined salivary gland EST libraries across medicinal leech lineages to experimentally and statistically evaluate whether evolutionary selection on peptides can identify structure-function activities of known therapeutically relevant bioactive compounds like antithrombin, hirudin and antistasin. The combined information content of a well corroborated leech phylogeny and broad taxonomic coverage of expressed proteins leads to a rich understanding of evolution and function in leech history. SN - 1469-8161 UR - https://www.unboundmedicine.com/medline/citation/21729354/Bacterial_symbiont_and_salivary_peptide_evolution_in_the_context_of_leech_phylogeny_ L2 - https://www.cambridge.org/core/product/identifier/S0031182011000539/type/journal_article DB - PRIME DP - Unbound Medicine ER -