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Compatibility of H9N2 avian influenza surface genes and 2009 pandemic H1N1 internal genes for transmission in the ferret model.
Proc Natl Acad Sci U S A. 2011 Jul 19; 108(29):12084-8.PN

Abstract

In 2009, a novel H1N1 influenza (pH1N1) virus caused the first influenza pandemic in 40 y. The virus was identified as a triple reassortant between avian, swine, and human influenza viruses, highlighting the importance of reassortment in the generation of viruses with pandemic potential. Previously, we showed that a reassortant virus composed of wild-type avian H9N2 surface genes in a seasonal human H3N2 backbone could gain efficient respiratory droplet transmission in the ferret model. Here we determine the ability of the H9N2 surface genes in the context of the internal genes of a pH1N1 virus to efficiently transmit via respiratory droplets in ferrets. We generated reassorted viruses carrying the HA gene alone or in combination with the NA gene of a prototypical H9N2 virus in the background of a pH1N1 virus. Four reassortant viruses were generated, with three of them showing efficient respiratory droplet transmission. Differences in replication efficiency were observed for these viruses; however, the results clearly indicate that H9N2 avian influenza viruses and pH1N1 viruses, both of which have occasionally infected pigs, have the potential to reassort and generate novel viruses with respiratory transmission potential in mammals.

Authors+Show Affiliations

Department of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

21730147

Citation

Kimble, J Brian, et al. "Compatibility of H9N2 Avian Influenza Surface Genes and 2009 Pandemic H1N1 Internal Genes for Transmission in the Ferret Model." Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 29, 2011, pp. 12084-8.
Kimble JB, Sorrell E, Shao H, et al. Compatibility of H9N2 avian influenza surface genes and 2009 pandemic H1N1 internal genes for transmission in the ferret model. Proc Natl Acad Sci USA. 2011;108(29):12084-8.
Kimble, J. B., Sorrell, E., Shao, H., Martin, P. L., & Perez, D. R. (2011). Compatibility of H9N2 avian influenza surface genes and 2009 pandemic H1N1 internal genes for transmission in the ferret model. Proceedings of the National Academy of Sciences of the United States of America, 108(29), 12084-8. https://doi.org/10.1073/pnas.1108058108
Kimble JB, et al. Compatibility of H9N2 Avian Influenza Surface Genes and 2009 Pandemic H1N1 Internal Genes for Transmission in the Ferret Model. Proc Natl Acad Sci USA. 2011 Jul 19;108(29):12084-8. PubMed PMID: 21730147.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Compatibility of H9N2 avian influenza surface genes and 2009 pandemic H1N1 internal genes for transmission in the ferret model. AU - Kimble,J Brian, AU - Sorrell,Erin, AU - Shao,Hongxia, AU - Martin,Philip L, AU - Perez,Daniel Roberto, Y1 - 2011/07/05/ PY - 2011/7/7/entrez PY - 2011/7/7/pubmed PY - 2011/10/4/medline SP - 12084 EP - 8 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 108 IS - 29 N2 - In 2009, a novel H1N1 influenza (pH1N1) virus caused the first influenza pandemic in 40 y. The virus was identified as a triple reassortant between avian, swine, and human influenza viruses, highlighting the importance of reassortment in the generation of viruses with pandemic potential. Previously, we showed that a reassortant virus composed of wild-type avian H9N2 surface genes in a seasonal human H3N2 backbone could gain efficient respiratory droplet transmission in the ferret model. Here we determine the ability of the H9N2 surface genes in the context of the internal genes of a pH1N1 virus to efficiently transmit via respiratory droplets in ferrets. We generated reassorted viruses carrying the HA gene alone or in combination with the NA gene of a prototypical H9N2 virus in the background of a pH1N1 virus. Four reassortant viruses were generated, with three of them showing efficient respiratory droplet transmission. Differences in replication efficiency were observed for these viruses; however, the results clearly indicate that H9N2 avian influenza viruses and pH1N1 viruses, both of which have occasionally infected pigs, have the potential to reassort and generate novel viruses with respiratory transmission potential in mammals. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/21730147/Compatibility_of_H9N2_avian_influenza_surface_genes_and_2009_pandemic_H1N1_internal_genes_for_transmission_in_the_ferret_model_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=21730147 DB - PRIME DP - Unbound Medicine ER -