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Identification of the third type of PVL phage in ST59 methicillin-resistant Staphylococcus aureus (MRSA) strains.
FEMS Microbiol Lett 2011; 323(1):20-8FM

Abstract

The genes lukS-PV and lukF-PV for Panton-Valentine leukocidin (PVL) that confers high virulence to Staphylococcus aureus are located on the prophages (PVL phages) which have been classified into group 1 and 2 sfi21-like Siphoviridae. We report novel PVL phages lysogenized in ST59 methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in Japan (JCSC7247) and Taiwan (JCSC5967). The genomes of φ7247PVL and φ5967PVL showed more than 99% identity, and the regions containing the five genes located at both ends of the prophages, int (integrase), hol (holin), ami (amidase), lukS-PV, and lukF-PV, are highly homologous to extant PVL phages. The genes for the structural module are less homologous to these phages, but are highly homologous to non-PVL phages belonging to group 3 Sfi21-like Siphoviridae, for example φN315. Subsequent PCR identification and nucleotide sequencing of an additional 11 Taiwanese ST59 MRSA isolates suggested they all carry the same phage as φ5967PVL, which differed from φ7247PVL by a single base. This study adds evidence to the notion that novel PVL phages would be generated through illegitimate recombination events by acquiring the region at which hol, ami, luk, and int genes would line up upon lytic growth, and suggests that the PVL-positive MRSA clones that have emerged worldwide may carry distinct phages.

Authors+Show Affiliations

Department of Infection Control Science, Graduate School of Medicine, Juntendo University, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21732964

Citation

Zhang, Meng, et al. "Identification of the Third Type of PVL Phage in ST59 Methicillin-resistant Staphylococcus Aureus (MRSA) Strains." FEMS Microbiology Letters, vol. 323, no. 1, 2011, pp. 20-8.
Zhang M, Ito T, Li S, et al. Identification of the third type of PVL phage in ST59 methicillin-resistant Staphylococcus aureus (MRSA) strains. FEMS Microbiol Lett. 2011;323(1):20-8.
Zhang, M., Ito, T., Li, S., Jin, J., Takeuchi, F., Lauderdale, T. L., ... Hiramatsu, K. (2011). Identification of the third type of PVL phage in ST59 methicillin-resistant Staphylococcus aureus (MRSA) strains. FEMS Microbiology Letters, 323(1), pp. 20-8. doi:10.1111/j.1574-6968.2011.02355.x.
Zhang M, et al. Identification of the Third Type of PVL Phage in ST59 Methicillin-resistant Staphylococcus Aureus (MRSA) Strains. FEMS Microbiol Lett. 2011;323(1):20-8. PubMed PMID: 21732964.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of the third type of PVL phage in ST59 methicillin-resistant Staphylococcus aureus (MRSA) strains. AU - Zhang,Meng, AU - Ito,Teruyo, AU - Li,Shanshuang, AU - Jin,Jingxun, AU - Takeuchi,Fumihiko, AU - Lauderdale,Tsai-Ling Yang, AU - Higashide,Masato, AU - Hiramatsu,Keiichi, Y1 - 2011/08/09/ PY - 2011/7/8/entrez PY - 2011/7/8/pubmed PY - 2012/3/8/medline SP - 20 EP - 8 JF - FEMS microbiology letters JO - FEMS Microbiol. Lett. VL - 323 IS - 1 N2 - The genes lukS-PV and lukF-PV for Panton-Valentine leukocidin (PVL) that confers high virulence to Staphylococcus aureus are located on the prophages (PVL phages) which have been classified into group 1 and 2 sfi21-like Siphoviridae. We report novel PVL phages lysogenized in ST59 methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in Japan (JCSC7247) and Taiwan (JCSC5967). The genomes of φ7247PVL and φ5967PVL showed more than 99% identity, and the regions containing the five genes located at both ends of the prophages, int (integrase), hol (holin), ami (amidase), lukS-PV, and lukF-PV, are highly homologous to extant PVL phages. The genes for the structural module are less homologous to these phages, but are highly homologous to non-PVL phages belonging to group 3 Sfi21-like Siphoviridae, for example φN315. Subsequent PCR identification and nucleotide sequencing of an additional 11 Taiwanese ST59 MRSA isolates suggested they all carry the same phage as φ5967PVL, which differed from φ7247PVL by a single base. This study adds evidence to the notion that novel PVL phages would be generated through illegitimate recombination events by acquiring the region at which hol, ami, luk, and int genes would line up upon lytic growth, and suggests that the PVL-positive MRSA clones that have emerged worldwide may carry distinct phages. SN - 1574-6968 UR - https://www.unboundmedicine.com/medline/citation/21732964/Identification_of_the_third_type_of_PVL_phage_in_ST59_methicillin_resistant_Staphylococcus_aureus__MRSA__strains_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2011.02355.x DB - PRIME DP - Unbound Medicine ER -