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SNP and mRNA expression for glutathione peroxidase 4 in Kashin-Beck disease.
Br J Nutr. 2012 Jan; 107(2):164-9.BJ

Abstract

Kashin-Beck disease (KBD) is a chronic endemic osteoarthropathy, which mainly occurs in West and Northeast China. Epidemiological studies suggest that Se deficiency is an important environmental factor for the incidence of KBD. Glutathione peroxidase 4 (GPx4) belongs to the glutathione peroxidase family, which is crucial for optimal antioxidant defences. Our purpose is to investigate the putative association between GPx4 polymorphisms and the risk of KBD. Restriction fragment length polymorphism-PCR was used to detect two SNP (rs713041, rs4807542) in 219 cases and 194 controls in Han Chinese subjects, and quantitative analysis for the GPx4 mRNA level was performed by the real-time PCR method. The results revealed that linkage disequilibrium existed in the two SNP. A significant difference was observed in the haplotype A-T (P = 0·0066) of GPx4, which was obviously lower in the KBD cases (0·006 v. 0·032 %). Correlation analysis based on a single locus showed no association between each SNP and KBD risk. Furthermore, the GPx4 mRNA level was dramatically lower in the blood of KBD patients. Overall, our finding indicated GPx4 polymorphisms and decreased mRNA level may be related to the development of KBD in the Chinese population, suggesting GPx4 as a possible candidate susceptibility gene for KBD.

Authors+Show Affiliations

Key Laboratory of Environment and Genes Related to Diseases of Education Ministry, College of Medicine, Institute of Endemic Disease, Xi'an Jiaotong University, No. 76 Yanta West Road, Xi'an, Shaanxi 710061, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21733339

Citation

Du, Xiao Hong, et al. "SNP and mRNA Expression for Glutathione Peroxidase 4 in Kashin-Beck Disease." The British Journal of Nutrition, vol. 107, no. 2, 2012, pp. 164-9.
Du XH, Dai XX, Xia Song R, et al. SNP and mRNA expression for glutathione peroxidase 4 in Kashin-Beck disease. Br J Nutr. 2012;107(2):164-9.
Du, X. H., Dai, X. X., Xia Song, R., Zou, X. Z., Yan Sun, W., Mo, X. Y., Lu Bai, G., & Xiong, Y. M. (2012). SNP and mRNA expression for glutathione peroxidase 4 in Kashin-Beck disease. The British Journal of Nutrition, 107(2), 164-9. https://doi.org/10.1017/S0007114511002704
Du XH, et al. SNP and mRNA Expression for Glutathione Peroxidase 4 in Kashin-Beck Disease. Br J Nutr. 2012;107(2):164-9. PubMed PMID: 21733339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SNP and mRNA expression for glutathione peroxidase 4 in Kashin-Beck disease. AU - Du,Xiao Hong, AU - Dai,Xiao Xia, AU - Xia Song,Rui, AU - Zou,Xiu Zhen, AU - Yan Sun,Wen, AU - Mo,Xiao Yan, AU - Lu Bai,Guang, AU - Xiong,Yong Min, Y1 - 2011/06/23/ PY - 2011/7/8/entrez PY - 2011/7/8/pubmed PY - 2012/3/3/medline SP - 164 EP - 9 JF - The British journal of nutrition JO - Br J Nutr VL - 107 IS - 2 N2 - Kashin-Beck disease (KBD) is a chronic endemic osteoarthropathy, which mainly occurs in West and Northeast China. Epidemiological studies suggest that Se deficiency is an important environmental factor for the incidence of KBD. Glutathione peroxidase 4 (GPx4) belongs to the glutathione peroxidase family, which is crucial for optimal antioxidant defences. Our purpose is to investigate the putative association between GPx4 polymorphisms and the risk of KBD. Restriction fragment length polymorphism-PCR was used to detect two SNP (rs713041, rs4807542) in 219 cases and 194 controls in Han Chinese subjects, and quantitative analysis for the GPx4 mRNA level was performed by the real-time PCR method. The results revealed that linkage disequilibrium existed in the two SNP. A significant difference was observed in the haplotype A-T (P = 0·0066) of GPx4, which was obviously lower in the KBD cases (0·006 v. 0·032 %). Correlation analysis based on a single locus showed no association between each SNP and KBD risk. Furthermore, the GPx4 mRNA level was dramatically lower in the blood of KBD patients. Overall, our finding indicated GPx4 polymorphisms and decreased mRNA level may be related to the development of KBD in the Chinese population, suggesting GPx4 as a possible candidate susceptibility gene for KBD. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/21733339/SNP_and_mRNA_expression_for_glutathione_peroxidase_4_in_Kashin_Beck_disease_ L2 - https://www.cambridge.org/core/product/identifier/S0007114511002704/type/journal_article DB - PRIME DP - Unbound Medicine ER -