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Continuous hypoxia attenuates paraquat-induced cytotoxicity in the human A549 lung carcinoma cell line.
Exp Mol Med. 2011 Sep 30; 43(9):494-500.EM

Abstract

Paraquat (1,1'-dimethyl-4,4'-bipyridinium dichloride; PQ), an effective and widely used herbicide, was commercially introduced in 1962. It is reduced by the electron donor NADPH, and then reduced PQ transfers the electrons to molecular oxygen, resulting in the production of reactive oxygen species (ROS), which are related to cellular toxicity. However, the influence of continuous hypoxia on PQ-induced ROS production has not fully been investigated. We evaluated in vitro the protective effect of continuous hypoxia on PQ-induced cytotoxicity in the human carcinogenic alveolar basal epithelial cell line (A549 cells) by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and live and dead assay, and by measuring lactate dehydrogenase (LDH) release. To elucidate the mechanism underlying this effect, we monitored the immunofluorescence of intracellular ROS and measured malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities. Continuous hypoxia protected the A549 cells from PQ-induced cytotoxicity. Continuous hypoxia for a period of 24 h significantly reduced intracellular ROS, decreased MDA concentration in the supernatant, and normalized SOD and GPx activities. Continuous hypoxia attenuated PQ-induced cell toxicity in A549 cells. This protective effect might be attributable to the suppression of PQ-induced ROS generation.

Authors+Show Affiliations

Department of Emergency Medicine, Chungbuk National University College of Medicine, Cheongju 361-763, Korea. nichekh2000@chungbuk.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21734449

Citation

Kim, Hoon, et al. "Continuous Hypoxia Attenuates Paraquat-induced Cytotoxicity in the Human A549 Lung Carcinoma Cell Line." Experimental & Molecular Medicine, vol. 43, no. 9, 2011, pp. 494-500.
Kim H, Lee SW, Baek KM, et al. Continuous hypoxia attenuates paraquat-induced cytotoxicity in the human A549 lung carcinoma cell line. Exp Mol Med. 2011;43(9):494-500.
Kim, H., Lee, S. W., Baek, K. M., Park, J. S., & Min, J. H. (2011). Continuous hypoxia attenuates paraquat-induced cytotoxicity in the human A549 lung carcinoma cell line. Experimental & Molecular Medicine, 43(9), 494-500.
Kim H, et al. Continuous Hypoxia Attenuates Paraquat-induced Cytotoxicity in the Human A549 Lung Carcinoma Cell Line. Exp Mol Med. 2011 Sep 30;43(9):494-500. PubMed PMID: 21734449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Continuous hypoxia attenuates paraquat-induced cytotoxicity in the human A549 lung carcinoma cell line. AU - Kim,Hoon, AU - Lee,Suk Woo, AU - Baek,Kyung Min, AU - Park,Jung Soo, AU - Min,Jin Hong, PY - 2011/7/8/entrez PY - 2011/7/8/pubmed PY - 2012/2/4/medline SP - 494 EP - 500 JF - Experimental & molecular medicine JO - Exp Mol Med VL - 43 IS - 9 N2 - Paraquat (1,1'-dimethyl-4,4'-bipyridinium dichloride; PQ), an effective and widely used herbicide, was commercially introduced in 1962. It is reduced by the electron donor NADPH, and then reduced PQ transfers the electrons to molecular oxygen, resulting in the production of reactive oxygen species (ROS), which are related to cellular toxicity. However, the influence of continuous hypoxia on PQ-induced ROS production has not fully been investigated. We evaluated in vitro the protective effect of continuous hypoxia on PQ-induced cytotoxicity in the human carcinogenic alveolar basal epithelial cell line (A549 cells) by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and live and dead assay, and by measuring lactate dehydrogenase (LDH) release. To elucidate the mechanism underlying this effect, we monitored the immunofluorescence of intracellular ROS and measured malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities. Continuous hypoxia protected the A549 cells from PQ-induced cytotoxicity. Continuous hypoxia for a period of 24 h significantly reduced intracellular ROS, decreased MDA concentration in the supernatant, and normalized SOD and GPx activities. Continuous hypoxia attenuated PQ-induced cell toxicity in A549 cells. This protective effect might be attributable to the suppression of PQ-induced ROS generation. SN - 2092-6413 UR - https://www.unboundmedicine.com/medline/citation/21734449/Continuous_hypoxia_attenuates_paraquat_induced_cytotoxicity_in_the_human_A549_lung_carcinoma_cell_line_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21734449/ DB - PRIME DP - Unbound Medicine ER -