Tags

Type your tag names separated by a space and hit enter

CuO and ZnO nanoparticles differently affect the secretion of fluorescent siderophores in the beneficial root colonizer, Pseudomonas chlororaphis O6.
Nanotoxicology. 2012 Sep; 6(6):635-42.N

Abstract

To understand the impact of environmental deposition of CuO and ZnO nanoparticles (NPs) on the production of bacterial metabolites, we examined the effects of their sub-lethal levels on the production of a pyoverdine (PVD) siderophore in a plant-beneficial bacterium, Pseudomonas chlororaphis O6 (PcO6). The NPs were characterized for size, shape, surface charge and ion release. Production of PVD by the cells was evaluated by fluorimetry and the expression of genes for PVD biosynthesis and export. The level of PVD in the presence of ZnO NPs was similar to that with Zn ions at 24 h, but the NPs maintained higher level than control at 48 h. In contrast, the reduction in PVD by CuO NPs was not duplicated by Cu ions. Expression of a gene encoding an inner membrane PVD transporter was inhibited by CuO NPs, supporting the observed low amounts of external PVD detected in those cells. These findings revealed NP-specific effect for CuO NPs on PcO6 metabolism, while for ZnO NPs, the release of ions was important. Because siderophores are part of the chemical communication between soil microbes and plants, the outcomes of plant-microbe interactions could be affected by responses to NPs.

Authors+Show Affiliations

Department of Biological Engineering, Utah State University, Logan, UT 84322, USA. cdimkpa@usu.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21740347

Citation

Dimkpa, Christian O., et al. "CuO and ZnO Nanoparticles Differently Affect the Secretion of Fluorescent Siderophores in the Beneficial Root Colonizer, Pseudomonas Chlororaphis O6." Nanotoxicology, vol. 6, no. 6, 2012, pp. 635-42.
Dimkpa CO, McLean JE, Britt DW, et al. CuO and ZnO nanoparticles differently affect the secretion of fluorescent siderophores in the beneficial root colonizer, Pseudomonas chlororaphis O6. Nanotoxicology. 2012;6(6):635-42.
Dimkpa, C. O., McLean, J. E., Britt, D. W., & Anderson, A. J. (2012). CuO and ZnO nanoparticles differently affect the secretion of fluorescent siderophores in the beneficial root colonizer, Pseudomonas chlororaphis O6. Nanotoxicology, 6(6), 635-42. https://doi.org/10.3109/17435390.2011.598246
Dimkpa CO, et al. CuO and ZnO Nanoparticles Differently Affect the Secretion of Fluorescent Siderophores in the Beneficial Root Colonizer, Pseudomonas Chlororaphis O6. Nanotoxicology. 2012;6(6):635-42. PubMed PMID: 21740347.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CuO and ZnO nanoparticles differently affect the secretion of fluorescent siderophores in the beneficial root colonizer, Pseudomonas chlororaphis O6. AU - Dimkpa,Christian O, AU - McLean,Joan E, AU - Britt,David W, AU - Anderson,Anne J, Y1 - 2011/07/11/ PY - 2011/7/12/entrez PY - 2011/7/12/pubmed PY - 2012/12/18/medline SP - 635 EP - 42 JF - Nanotoxicology JO - Nanotoxicology VL - 6 IS - 6 N2 - To understand the impact of environmental deposition of CuO and ZnO nanoparticles (NPs) on the production of bacterial metabolites, we examined the effects of their sub-lethal levels on the production of a pyoverdine (PVD) siderophore in a plant-beneficial bacterium, Pseudomonas chlororaphis O6 (PcO6). The NPs were characterized for size, shape, surface charge and ion release. Production of PVD by the cells was evaluated by fluorimetry and the expression of genes for PVD biosynthesis and export. The level of PVD in the presence of ZnO NPs was similar to that with Zn ions at 24 h, but the NPs maintained higher level than control at 48 h. In contrast, the reduction in PVD by CuO NPs was not duplicated by Cu ions. Expression of a gene encoding an inner membrane PVD transporter was inhibited by CuO NPs, supporting the observed low amounts of external PVD detected in those cells. These findings revealed NP-specific effect for CuO NPs on PcO6 metabolism, while for ZnO NPs, the release of ions was important. Because siderophores are part of the chemical communication between soil microbes and plants, the outcomes of plant-microbe interactions could be affected by responses to NPs. SN - 1743-5404 UR - https://www.unboundmedicine.com/medline/citation/21740347/CuO_and_ZnO_nanoparticles_differently_affect_the_secretion_of_fluorescent_siderophores_in_the_beneficial_root_colonizer_Pseudomonas_chlororaphis_O6_ L2 - http://www.tandfonline.com/doi/full/10.3109/17435390.2011.598246 DB - PRIME DP - Unbound Medicine ER -