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The Arabidopsis thaliana cysteine-rich receptor-like kinase CRK20 modulates host responses to Pseudomonas syringae pv. tomato DC3000 infection.
J Plant Physiol. 2011 Oct 15; 168(15):1784-94.JP

Abstract

In plants, the cysteine-rich repeat kinases (CRKs) are a sub-family of receptor-like protein kinases that contain the DUF26 motif in their extracellular domains. It has been shown that in Arabidopsis thaliana, CRK20 is transcriptionally induced by pathogens, salicylic acid and ozone (O(3)). However, its role in responses to biotic and abiotic stress remains to be elucidated. To determine the function of CRK20 in such responses, two CRK20 loss-of-function mutants, crk20-1 and crk20-2, were isolated from public collections of Arabidopsis T-DNA tagged lines and examined for responses to O(3) and Pseudomonas syringae pv. tomato (Pst) DC3000. crk20-1 and crk20-2 showed similar O(3) sensitivities and no differences in the expression of defense genes when compared with the wild-type. However, pathogen growth was significantly reduced, while there were no differences in the induction of salicylic acid related defense genes or salicylic acid accumulation. Furthermore, correlation analysis of CRK20 gene expression suggests that it has a role in the control of H(2)O and/or nutrient transport. We therefore propose that CRK20 promotes conditions that are favorable for Pst DC3000 growth in Arabidopsis, possibly through the regulation of apoplastic homeostasis, and consequently, of the environment of this biotrophic pathogen.

Authors+Show Affiliations

Department of Applied Biology, University of Perugia, Borgo XX Giugno, 74, I-06121 Perugia, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21742407

Citation

Ederli, Luisa, et al. "The Arabidopsis Thaliana Cysteine-rich Receptor-like Kinase CRK20 Modulates Host Responses to Pseudomonas Syringae Pv. Tomato DC3000 Infection." Journal of Plant Physiology, vol. 168, no. 15, 2011, pp. 1784-94.
Ederli L, Madeo L, Calderini O, et al. The Arabidopsis thaliana cysteine-rich receptor-like kinase CRK20 modulates host responses to Pseudomonas syringae pv. tomato DC3000 infection. J Plant Physiol. 2011;168(15):1784-94.
Ederli, L., Madeo, L., Calderini, O., Gehring, C., Moretti, C., Buonaurio, R., Paolocci, F., & Pasqualini, S. (2011). The Arabidopsis thaliana cysteine-rich receptor-like kinase CRK20 modulates host responses to Pseudomonas syringae pv. tomato DC3000 infection. Journal of Plant Physiology, 168(15), 1784-94. https://doi.org/10.1016/j.jplph.2011.05.018
Ederli L, et al. The Arabidopsis Thaliana Cysteine-rich Receptor-like Kinase CRK20 Modulates Host Responses to Pseudomonas Syringae Pv. Tomato DC3000 Infection. J Plant Physiol. 2011 Oct 15;168(15):1784-94. PubMed PMID: 21742407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Arabidopsis thaliana cysteine-rich receptor-like kinase CRK20 modulates host responses to Pseudomonas syringae pv. tomato DC3000 infection. AU - Ederli,Luisa, AU - Madeo,Laura, AU - Calderini,Ornella, AU - Gehring,Chris, AU - Moretti,Chiaraluce, AU - Buonaurio,Roberto, AU - Paolocci,Francesco, AU - Pasqualini,Stefania, Y1 - 2011/07/13/ PY - 2011/02/01/received PY - 2011/03/31/revised PY - 2011/05/03/accepted PY - 2011/7/12/entrez PY - 2011/7/12/pubmed PY - 2013/5/4/medline SP - 1784 EP - 94 JF - Journal of plant physiology JO - J. Plant Physiol. VL - 168 IS - 15 N2 - In plants, the cysteine-rich repeat kinases (CRKs) are a sub-family of receptor-like protein kinases that contain the DUF26 motif in their extracellular domains. It has been shown that in Arabidopsis thaliana, CRK20 is transcriptionally induced by pathogens, salicylic acid and ozone (O(3)). However, its role in responses to biotic and abiotic stress remains to be elucidated. To determine the function of CRK20 in such responses, two CRK20 loss-of-function mutants, crk20-1 and crk20-2, were isolated from public collections of Arabidopsis T-DNA tagged lines and examined for responses to O(3) and Pseudomonas syringae pv. tomato (Pst) DC3000. crk20-1 and crk20-2 showed similar O(3) sensitivities and no differences in the expression of defense genes when compared with the wild-type. However, pathogen growth was significantly reduced, while there were no differences in the induction of salicylic acid related defense genes or salicylic acid accumulation. Furthermore, correlation analysis of CRK20 gene expression suggests that it has a role in the control of H(2)O and/or nutrient transport. We therefore propose that CRK20 promotes conditions that are favorable for Pst DC3000 growth in Arabidopsis, possibly through the regulation of apoplastic homeostasis, and consequently, of the environment of this biotrophic pathogen. SN - 1618-1328 UR - https://www.unboundmedicine.com/medline/citation/21742407/The_Arabidopsis_thaliana_cysteine_rich_receptor_like_kinase_CRK20_modulates_host_responses_to_Pseudomonas_syringae_pv__tomato_DC3000_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0176-1617(11)00277-X DB - PRIME DP - Unbound Medicine ER -