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Vitreoscilla hemoglobin expression in engineered Escherichia coli: improved performance in high cell-density batch cultivations.
Biotechnol J. 2011 Aug; 6(8):993-1002.BJ

Abstract

High cell-density cultivations are the preferred system for biomolecules production by Escherichia coli. It has been previously demonstrated that a strain of E. coli with a modified substrate transport system is able to attain high cell densities in batch mode, due to the very low overflow metabolism displayed. The use of elevated amounts of glucose from the beginning of the cultivation, eliminates the existence of substrate gradients due to deficient mixing at large-scale. However, the large amounts of oxygen demanded resulted in microaerobic conditions after some hours of cultivation, even at small-scale. In this work, the effect of expressing the Vitreoscilla hemoglobin (VHb) in the engineered strain during batch cultures using high-glucose concentrations was tested. Together, the expression of VHb and the modified substrate transport system resulted in a 33% increase of biomass production compared to the parental strain (W3110) lacking the VHb in batch cultivations using 25 g/L of glucose. When 50 g/L of glucose were used, expression of VHb in the modified strain led to 11% higher biomass production compared to W3110. The VHb also increased the growth rates of the strains by about 30% in the aerobic phase and more than 200% in the microaerobic phase of batch cultivation.

Authors+Show Affiliations

Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, México, D.F.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21744499

Citation

Pablos, Tania E., et al. "Vitreoscilla Hemoglobin Expression in Engineered Escherichia Coli: Improved Performance in High Cell-density Batch Cultivations." Biotechnology Journal, vol. 6, no. 8, 2011, pp. 993-1002.
Pablos TE, Mora EM, Le Borgne S, et al. Vitreoscilla hemoglobin expression in engineered Escherichia coli: improved performance in high cell-density batch cultivations. Biotechnol J. 2011;6(8):993-1002.
Pablos, T. E., Mora, E. M., Le Borgne, S., Ramírez, O. T., Gosset, G., & Lara, A. R. (2011). Vitreoscilla hemoglobin expression in engineered Escherichia coli: improved performance in high cell-density batch cultivations. Biotechnology Journal, 6(8), 993-1002. https://doi.org/10.1002/biot.201000405
Pablos TE, et al. Vitreoscilla Hemoglobin Expression in Engineered Escherichia Coli: Improved Performance in High Cell-density Batch Cultivations. Biotechnol J. 2011;6(8):993-1002. PubMed PMID: 21744499.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vitreoscilla hemoglobin expression in engineered Escherichia coli: improved performance in high cell-density batch cultivations. AU - Pablos,Tania E, AU - Mora,Eugenio Meza, AU - Le Borgne,Sylvie, AU - Ramírez,Octavio T, AU - Gosset,Guillermo, AU - Lara,Alvaro R, Y1 - 2011/07/11/ PY - 2011/02/28/received PY - 2011/04/29/revised PY - 2011/05/17/accepted PY - 2011/7/12/entrez PY - 2011/7/12/pubmed PY - 2011/12/13/medline SP - 993 EP - 1002 JF - Biotechnology journal JO - Biotechnol J VL - 6 IS - 8 N2 - High cell-density cultivations are the preferred system for biomolecules production by Escherichia coli. It has been previously demonstrated that a strain of E. coli with a modified substrate transport system is able to attain high cell densities in batch mode, due to the very low overflow metabolism displayed. The use of elevated amounts of glucose from the beginning of the cultivation, eliminates the existence of substrate gradients due to deficient mixing at large-scale. However, the large amounts of oxygen demanded resulted in microaerobic conditions after some hours of cultivation, even at small-scale. In this work, the effect of expressing the Vitreoscilla hemoglobin (VHb) in the engineered strain during batch cultures using high-glucose concentrations was tested. Together, the expression of VHb and the modified substrate transport system resulted in a 33% increase of biomass production compared to the parental strain (W3110) lacking the VHb in batch cultivations using 25 g/L of glucose. When 50 g/L of glucose were used, expression of VHb in the modified strain led to 11% higher biomass production compared to W3110. The VHb also increased the growth rates of the strains by about 30% in the aerobic phase and more than 200% in the microaerobic phase of batch cultivation. SN - 1860-7314 UR - https://www.unboundmedicine.com/medline/citation/21744499/Vitreoscilla_hemoglobin_expression_in_engineered_Escherichia_coli:_improved_performance_in_high_cell_density_batch_cultivations_ L2 - https://doi.org/10.1002/biot.201000405 DB - PRIME DP - Unbound Medicine ER -