Tags

Type your tag names separated by a space and hit enter

Pharmacokinetic study of lappaconitine hydrobromide in mice by LC-MS.
Yao Xue Xue Bao. 2011 Apr; 46(4):432-7.YX

Abstract

A high sensitive and rapid method was developed for the analysis of lappaconitine in mouse plasma using liquid chromatography coupled to mass spectrometry (LC-MS). Detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode, monitoring the transitions m/z 585 --> m/z 535 and m/z 356 --> m/z 192, for the quantification of lappaconitine and tetrahydropalmatine (internal standard, IS), respectively. The method was linear over the concentration range of 3.0-2000.0 ng x mL(-1). The lower limit of quantification was 3.0 ng x mL(-1). Intra- and inter-run precisions (RSD) were both less than 9.9% and accuracy (RE) within +/- 4.8%. After single intravenous injections of lappaconitine hydrobromide at 1.0, 2.0 and 4.0 mg x kg(-1), the elimination half-lives (t(1/2)) were 0.47, 0.48 and 0.49 h, and the areas under the curve (AUC(0-t)) were 55.5, 110.5 and 402.9 ng x h x mL(-1), separately. The pharmacokinetic profile of lappaconitine was linear at relatively lower dose levels (1.0-2.0 mg x kg(-1)). When the dose increased farther to 4.0 mg x kg(-1), the Vz and CL decreased, and the increase fold of the AUC was much larger than that of the dose.

Authors+Show Affiliations

School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21748973

Citation

Wang, Qing, et al. "Pharmacokinetic Study of Lappaconitine Hydrobromide in Mice By LC-MS." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 46, no. 4, 2011, pp. 432-7.
Wang Q, Li ZJ, Sun L, et al. Pharmacokinetic study of lappaconitine hydrobromide in mice by LC-MS. Yao Xue Xue Bao. 2011;46(4):432-7.
Wang, Q., Li, Z. J., Sun, L., Gao, L. Y., Li, M. H., Hao, J. J., Zhang, X., & Sun, Y. M. (2011). Pharmacokinetic study of lappaconitine hydrobromide in mice by LC-MS. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 46(4), 432-7.
Wang Q, et al. Pharmacokinetic Study of Lappaconitine Hydrobromide in Mice By LC-MS. Yao Xue Xue Bao. 2011;46(4):432-7. PubMed PMID: 21748973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pharmacokinetic study of lappaconitine hydrobromide in mice by LC-MS. AU - Wang,Qing, AU - Li,Zi-jing, AU - Sun,Lu, AU - Gao,Li-ying, AU - Li,Ming-hui, AU - Hao,Jia-jia, AU - Zhang,Xin, AU - Sun,Yu-ming, PY - 2011/7/14/entrez PY - 2011/7/14/pubmed PY - 2013/2/26/medline SP - 432 EP - 7 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 46 IS - 4 N2 - A high sensitive and rapid method was developed for the analysis of lappaconitine in mouse plasma using liquid chromatography coupled to mass spectrometry (LC-MS). Detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode, monitoring the transitions m/z 585 --> m/z 535 and m/z 356 --> m/z 192, for the quantification of lappaconitine and tetrahydropalmatine (internal standard, IS), respectively. The method was linear over the concentration range of 3.0-2000.0 ng x mL(-1). The lower limit of quantification was 3.0 ng x mL(-1). Intra- and inter-run precisions (RSD) were both less than 9.9% and accuracy (RE) within +/- 4.8%. After single intravenous injections of lappaconitine hydrobromide at 1.0, 2.0 and 4.0 mg x kg(-1), the elimination half-lives (t(1/2)) were 0.47, 0.48 and 0.49 h, and the areas under the curve (AUC(0-t)) were 55.5, 110.5 and 402.9 ng x h x mL(-1), separately. The pharmacokinetic profile of lappaconitine was linear at relatively lower dose levels (1.0-2.0 mg x kg(-1)). When the dose increased farther to 4.0 mg x kg(-1), the Vz and CL decreased, and the increase fold of the AUC was much larger than that of the dose. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/21748973/Pharmacokinetic_study_of_lappaconitine_hydrobromide_in_mice_by_LC_MS_ L2 - https://medlineplus.gov/painrelievers.html DB - PRIME DP - Unbound Medicine ER -