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A single exposure to iron oxide nanoparticles attenuates antigen-specific antibody production and T-cell reactivity in ovalbumin-sensitized BALB/c mice.
Int J Nanomedicine. 2011; 6:1229-35.IJ

Abstract

BACKGROUND

Superparamagnetic iron oxide nanoparticles have been used in clinical applications as a diagnostic contrasting agent. Previous studies showed that iron oxide nanoparticles deposited in the liver and spleen after systemic administration. The present study investigated the effect of iron oxide nanoparticles on antigen-specific immune responses in mice sensitized with the T cell-dependent antigen ovalbumin (OVA).

METHODS

BALB/c mice were intravenously administered with a single dose of iron oxide nanoparticles (10-60 mg Fe/kg) 1 hour prior to OVA sensitization, and the serum antibody production and splenocyte reactivity were examined 7 days later.

RESULTS

The serum levels of OVA-specific IgG(1) and IgG(2a) were significantly attenuated by treatment with iron oxide nanoparticles. The production of interferon-γ and interleukin-4 by splenocytes re-stimulated with OVA in culture was robustly suppressed in mice administered with iron oxide nanoparticles. The viability of OVA-stimulated splenocytes was also attenuated. In contrast, treatment with iron oxide nanoparticles did not affect the viability of splenocytes stimulated with concanavalin A, a T-cell mitogen.

CONCLUSION

Collectively, these data indicate that systemic exposure to a single dose of iron oxide nanoparticles compromises subsequent antigen-specific immune reactions, including the serum production of antigen-specific antibodies, and the functionality of T cells.

Authors+Show Affiliations

Department and Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21753874

Citation

Shen, Chien-Chang, et al. "A Single Exposure to Iron Oxide Nanoparticles Attenuates Antigen-specific Antibody Production and T-cell Reactivity in Ovalbumin-sensitized BALB/c Mice." International Journal of Nanomedicine, vol. 6, 2011, pp. 1229-35.
Shen CC, Wang CC, Liao MH, et al. A single exposure to iron oxide nanoparticles attenuates antigen-specific antibody production and T-cell reactivity in ovalbumin-sensitized BALB/c mice. Int J Nanomedicine. 2011;6:1229-35.
Shen, C. C., Wang, C. C., Liao, M. H., & Jan, T. R. (2011). A single exposure to iron oxide nanoparticles attenuates antigen-specific antibody production and T-cell reactivity in ovalbumin-sensitized BALB/c mice. International Journal of Nanomedicine, 6, 1229-35. https://doi.org/10.2147/IJN.S21019
Shen CC, et al. A Single Exposure to Iron Oxide Nanoparticles Attenuates Antigen-specific Antibody Production and T-cell Reactivity in Ovalbumin-sensitized BALB/c Mice. Int J Nanomedicine. 2011;6:1229-35. PubMed PMID: 21753874.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A single exposure to iron oxide nanoparticles attenuates antigen-specific antibody production and T-cell reactivity in ovalbumin-sensitized BALB/c mice. AU - Shen,Chien-Chang, AU - Wang,Chia-Chi, AU - Liao,Mei-Hsiu, AU - Jan,Tong-Rong, Y1 - 2011/06/20/ PY - 2011/06/18/received PY - 2011/7/15/entrez PY - 2011/7/15/pubmed PY - 2011/11/4/medline KW - antigen-specific KW - immune KW - iron oxide nanoparticle KW - ovalbumin SP - 1229 EP - 35 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 6 N2 - BACKGROUND: Superparamagnetic iron oxide nanoparticles have been used in clinical applications as a diagnostic contrasting agent. Previous studies showed that iron oxide nanoparticles deposited in the liver and spleen after systemic administration. The present study investigated the effect of iron oxide nanoparticles on antigen-specific immune responses in mice sensitized with the T cell-dependent antigen ovalbumin (OVA). METHODS: BALB/c mice were intravenously administered with a single dose of iron oxide nanoparticles (10-60 mg Fe/kg) 1 hour prior to OVA sensitization, and the serum antibody production and splenocyte reactivity were examined 7 days later. RESULTS: The serum levels of OVA-specific IgG(1) and IgG(2a) were significantly attenuated by treatment with iron oxide nanoparticles. The production of interferon-γ and interleukin-4 by splenocytes re-stimulated with OVA in culture was robustly suppressed in mice administered with iron oxide nanoparticles. The viability of OVA-stimulated splenocytes was also attenuated. In contrast, treatment with iron oxide nanoparticles did not affect the viability of splenocytes stimulated with concanavalin A, a T-cell mitogen. CONCLUSION: Collectively, these data indicate that systemic exposure to a single dose of iron oxide nanoparticles compromises subsequent antigen-specific immune reactions, including the serum production of antigen-specific antibodies, and the functionality of T cells. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/21753874/A_single_exposure_to_iron_oxide_nanoparticles_attenuates_antigen_specific_antibody_production_and_T_cell_reactivity_in_ovalbumin_sensitized_BALB/c_mice_ L2 - https://dx.doi.org/10.2147/IJN.S21019 DB - PRIME DP - Unbound Medicine ER -