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Curculigoside A attenuates experimental cerebral ischemia injury in vitro and vivo.
Neuroscience. 2011 Sep 29; 192:572-9.N

Abstract

Recent studies have demonstrated nuclear factor-κB (NF-κB) and high-mobility group box1 (HMGB1) associated with the pathophysiology of cerebral ischemia. We isolated Curculigoside A, the major bioactive compound present in Curculigo orchioides. The objectives of this study were to determine the effects of Curculigoside A on cultured neuronal cell line, SH-SY5Y in vitro and experimental ischemic stroke in vivo. For oxygen-glucose deprivation and tumor necrosis factor-α (TNF-α) stimulated SH-SY5Y cell line in vitro, SH-SY5Y cells were pre-incubated with Curculigoside A. For in vivo experiment, rats were subjected to middle cerebral artery occlusion (MCAO) for 1 h, then followed by reperfusion for 23 h. Treatment of SH-SY5Y cells with Curculigoside A reduced the oxygen-glucose deprivation-induced cytotoxicity and apoptosis, blocked TNF-α-induced NF-κB and IκB-α phosphorylation, and decreased HMGB1 expression. At doses higher than 10 mg/kg, Curculigoside A produced a significant neuroprotective potential in rats with ischemia and reperfusion (I/R). Curculigoside A (20 mg/kg) demonstrated significant neuroprotective activity even after delayed administration at 1 h, 3 h, and 5 h after I/R. Curculigoside A 20 mg/kg attenuated histopathological damage, decreased cerebral Evans Blue extravasation, inhibited NF-κB activation and reduced HMGB1 expression. These data show that Curculigoside A protects brain against I/R injury with a favorable therapeutic time-window by alleviating cerebral I/R injury and attenuating blood-brain barrier (BBB) breakdown, and its protective effects may involve HMGB1 and NF-κB signaling pathway.

Authors+Show Affiliations

School of Pharmacy, Yantai University, Yantai 264003, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21756977

Citation

Jiang, W, et al. "Curculigoside a Attenuates Experimental Cerebral Ischemia Injury in Vitro and Vivo." Neuroscience, vol. 192, 2011, pp. 572-9.
Jiang W, Fu F, Tian J, et al. Curculigoside A attenuates experimental cerebral ischemia injury in vitro and vivo. Neuroscience. 2011;192:572-9.
Jiang, W., Fu, F., Tian, J., Zhu, H., & Hou, J. (2011). Curculigoside A attenuates experimental cerebral ischemia injury in vitro and vivo. Neuroscience, 192, 572-9. https://doi.org/10.1016/j.neuroscience.2011.06.079
Jiang W, et al. Curculigoside a Attenuates Experimental Cerebral Ischemia Injury in Vitro and Vivo. Neuroscience. 2011 Sep 29;192:572-9. PubMed PMID: 21756977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Curculigoside A attenuates experimental cerebral ischemia injury in vitro and vivo. AU - Jiang,W, AU - Fu,F, AU - Tian,J, AU - Zhu,H, AU - Hou,J, Y1 - 2011/07/01/ PY - 2011/04/14/received PY - 2011/06/25/revised PY - 2011/06/28/accepted PY - 2011/7/16/entrez PY - 2011/7/16/pubmed PY - 2012/1/19/medline SP - 572 EP - 9 JF - Neuroscience JO - Neuroscience VL - 192 N2 - Recent studies have demonstrated nuclear factor-κB (NF-κB) and high-mobility group box1 (HMGB1) associated with the pathophysiology of cerebral ischemia. We isolated Curculigoside A, the major bioactive compound present in Curculigo orchioides. The objectives of this study were to determine the effects of Curculigoside A on cultured neuronal cell line, SH-SY5Y in vitro and experimental ischemic stroke in vivo. For oxygen-glucose deprivation and tumor necrosis factor-α (TNF-α) stimulated SH-SY5Y cell line in vitro, SH-SY5Y cells were pre-incubated with Curculigoside A. For in vivo experiment, rats were subjected to middle cerebral artery occlusion (MCAO) for 1 h, then followed by reperfusion for 23 h. Treatment of SH-SY5Y cells with Curculigoside A reduced the oxygen-glucose deprivation-induced cytotoxicity and apoptosis, blocked TNF-α-induced NF-κB and IκB-α phosphorylation, and decreased HMGB1 expression. At doses higher than 10 mg/kg, Curculigoside A produced a significant neuroprotective potential in rats with ischemia and reperfusion (I/R). Curculigoside A (20 mg/kg) demonstrated significant neuroprotective activity even after delayed administration at 1 h, 3 h, and 5 h after I/R. Curculigoside A 20 mg/kg attenuated histopathological damage, decreased cerebral Evans Blue extravasation, inhibited NF-κB activation and reduced HMGB1 expression. These data show that Curculigoside A protects brain against I/R injury with a favorable therapeutic time-window by alleviating cerebral I/R injury and attenuating blood-brain barrier (BBB) breakdown, and its protective effects may involve HMGB1 and NF-κB signaling pathway. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/21756977/Curculigoside_A_attenuates_experimental_cerebral_ischemia_injury_in_vitro_and_vivo_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(11)00789-5 DB - PRIME DP - Unbound Medicine ER -