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Regulation of Wnt/β-catenin signaling by CCAAT/enhancer binding protein β during adipogenesis.
Obesity (Silver Spring). 2012 Mar; 20(3):482-7.O

Abstract

Activation of the Wnt/β-catenin signaling pathway inhibits adipogenesis, while disruption of Wnt signaling leads to spontaneous adipogenesis. CCAAT/enhancer binding protein β (C/EBPβ) is rapidly induced in early stages of adipogenesis and is responsible for transcriptional induction of two major adipogenic transcription factors, peroxisome proliferator-activated receptor γ (PPARγ) and C/EBPα. In this study, we examined whether C/EBPβ is involved in the suppression of Wnt/β-catenin signaling during adipogenesis. Knockdown of C/EBPβ expression not only inhibited adipogenesis but also maintained active Wnt/β-catenin signaling, after addition of adipogenic inducers. In contrast, overexpression of C/EBPβ substantially inhibited Wnt signaling. Interestingly, our data showed that C/EBPβ is involved in the expression of Wnt10b, a major Wnt ligand in preadipocytes, even though C/EBPβ is not an essential factor to regulate Wnt10b expression during adipogenesis, and that C/EBPβ inhibits Wnt10b promoter activity by directly binding to specific regions of the promoter. These results suggest a dual function of C/EBPβ: stimulating expression of adipogenic genes and inhibiting Wnt signaling.

Authors+Show Affiliations

Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21760632

Citation

Chung, Sung Soo, et al. "Regulation of Wnt/β-catenin Signaling By CCAAT/enhancer Binding Protein Β During Adipogenesis." Obesity (Silver Spring, Md.), vol. 20, no. 3, 2012, pp. 482-7.
Chung SS, Lee JS, Kim M, et al. Regulation of Wnt/β-catenin signaling by CCAAT/enhancer binding protein β during adipogenesis. Obesity (Silver Spring). 2012;20(3):482-7.
Chung, S. S., Lee, J. S., Kim, M., Ahn, B. Y., Jung, H. S., Lee, H. M., Kim, J. W., & Park, K. S. (2012). Regulation of Wnt/β-catenin signaling by CCAAT/enhancer binding protein β during adipogenesis. Obesity (Silver Spring, Md.), 20(3), 482-7. https://doi.org/10.1038/oby.2011.212
Chung SS, et al. Regulation of Wnt/β-catenin Signaling By CCAAT/enhancer Binding Protein Β During Adipogenesis. Obesity (Silver Spring). 2012;20(3):482-7. PubMed PMID: 21760632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of Wnt/β-catenin signaling by CCAAT/enhancer binding protein β during adipogenesis. AU - Chung,Sung Soo, AU - Lee,Ji Seon, AU - Kim,Min, AU - Ahn,Byung Yong, AU - Jung,Hye Seung, AU - Lee,Hak Mo, AU - Kim,Jae-Woo, AU - Park,Kyong Soo, Y1 - 2011/07/14/ PY - 2011/7/16/entrez PY - 2011/7/16/pubmed PY - 2012/6/13/medline SP - 482 EP - 7 JF - Obesity (Silver Spring, Md.) JO - Obesity (Silver Spring) VL - 20 IS - 3 N2 - Activation of the Wnt/β-catenin signaling pathway inhibits adipogenesis, while disruption of Wnt signaling leads to spontaneous adipogenesis. CCAAT/enhancer binding protein β (C/EBPβ) is rapidly induced in early stages of adipogenesis and is responsible for transcriptional induction of two major adipogenic transcription factors, peroxisome proliferator-activated receptor γ (PPARγ) and C/EBPα. In this study, we examined whether C/EBPβ is involved in the suppression of Wnt/β-catenin signaling during adipogenesis. Knockdown of C/EBPβ expression not only inhibited adipogenesis but also maintained active Wnt/β-catenin signaling, after addition of adipogenic inducers. In contrast, overexpression of C/EBPβ substantially inhibited Wnt signaling. Interestingly, our data showed that C/EBPβ is involved in the expression of Wnt10b, a major Wnt ligand in preadipocytes, even though C/EBPβ is not an essential factor to regulate Wnt10b expression during adipogenesis, and that C/EBPβ inhibits Wnt10b promoter activity by directly binding to specific regions of the promoter. These results suggest a dual function of C/EBPβ: stimulating expression of adipogenic genes and inhibiting Wnt signaling. SN - 1930-739X UR - https://www.unboundmedicine.com/medline/citation/21760632/Regulation_of_Wnt/β_catenin_signaling_by_CCAAT/enhancer_binding_protein_β_during_adipogenesis_ DB - PRIME DP - Unbound Medicine ER -