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(1)H and DOSY NMR spectroscopy analysis of Ligusticum porteri rhizome extracts.
Magn Reson Chem. 2011 Aug; 49(8):469-76.MR

Abstract

The presence of dimeric phthalides and other constituents in extracts of the vegetal species Ligusticum porteri was established by NMR spectroscopy. In comparative qualitative (1)H NMR analyses of acetone extracts of rhizomes from fresh and dried L. porteri samples, we found that the dimeric phthalides tokinolide B (3), diligustilide (4) and riligustilide (5) were naturally produced by the plant and not post-harvest products. We also obtained DOSY (1)H NMR data that provided both virtual separation and structural information for the phthalides present in a dry acetone extract of L. porteri. In addition, we developed a protocol for the quantification of dimeric phthalides, which is performed by calculating the relative ratio of the peak area of selected proton signals for some compounds with respect to the known signal of the internal standard, 4-dimethylaminopyridine. The protocol allows the rapid and direct quantification of dimeric phthalides and others constituents in fresh L. porteri rhizomes.

Authors+Show Affiliations

Instituto de Química, Universidad Nacional Autónoma de México, Circuito Exterior, Ciudad Universitaria, Coyoacán, 04510, México, D.F.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21761449

Citation

León, Alejandra, et al. "(1)H and DOSY NMR Spectroscopy Analysis of Ligusticum Porteri Rhizome Extracts." Magnetic Resonance in Chemistry : MRC, vol. 49, no. 8, 2011, pp. 469-76.
León A, Chávez MI, Delgado G. (1)H and DOSY NMR spectroscopy analysis of Ligusticum porteri rhizome extracts. Magn Reson Chem. 2011;49(8):469-76.
León, A., Chávez, M. I., & Delgado, G. (2011). (1)H and DOSY NMR spectroscopy analysis of Ligusticum porteri rhizome extracts. Magnetic Resonance in Chemistry : MRC, 49(8), 469-76. https://doi.org/10.1002/mrc.2767
León A, Chávez MI, Delgado G. (1)H and DOSY NMR Spectroscopy Analysis of Ligusticum Porteri Rhizome Extracts. Magn Reson Chem. 2011;49(8):469-76. PubMed PMID: 21761449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - (1)H and DOSY NMR spectroscopy analysis of Ligusticum porteri rhizome extracts. AU - León,Alejandra, AU - Chávez,María Isabel, AU - Delgado,Guillermo, Y1 - 2011/07/14/ PY - 2010/11/25/received PY - 2011/04/12/revised PY - 2011/04/21/accepted PY - 2011/7/16/entrez PY - 2011/7/16/pubmed PY - 2015/7/1/medline KW - 1H KW - DOSY NMR KW - Ligusticum porteri KW - NMR KW - Z-ligustilide KW - coniferyl ferulate KW - diligustilide KW - dimeric phthalides KW - qNMR KW - quantification KW - riligustilide KW - tokinolide B SP - 469 EP - 76 JF - Magnetic resonance in chemistry : MRC JO - Magn Reson Chem VL - 49 IS - 8 N2 - The presence of dimeric phthalides and other constituents in extracts of the vegetal species Ligusticum porteri was established by NMR spectroscopy. In comparative qualitative (1)H NMR analyses of acetone extracts of rhizomes from fresh and dried L. porteri samples, we found that the dimeric phthalides tokinolide B (3), diligustilide (4) and riligustilide (5) were naturally produced by the plant and not post-harvest products. We also obtained DOSY (1)H NMR data that provided both virtual separation and structural information for the phthalides present in a dry acetone extract of L. porteri. In addition, we developed a protocol for the quantification of dimeric phthalides, which is performed by calculating the relative ratio of the peak area of selected proton signals for some compounds with respect to the known signal of the internal standard, 4-dimethylaminopyridine. The protocol allows the rapid and direct quantification of dimeric phthalides and others constituents in fresh L. porteri rhizomes. SN - 1097-458X UR - https://www.unboundmedicine.com/medline/citation/21761449/_1_H_and_DOSY_NMR_spectroscopy_analysis_of_Ligusticum_porteri_rhizome_extracts_ L2 - https://doi.org/10.1002/mrc.2767 DB - PRIME DP - Unbound Medicine ER -