Tags

Type your tag names separated by a space and hit enter

Telomere length is related to alternative splice patterns of telomerase in thyroid tumors.
Am J Pathol. 2011 Sep; 179(3):1415-24.AJ

Abstract

Telomere dysfunction and aberrant telomerase expression play important roles in tumorigenesis. In thyroid tumors, three possibly inhibitory splice variants of the active full-length isoform of human telomerase reverse transcriptase (hTERT) may be expressed. These variants might regulate telomerase activity and telomere length because it is the fraction of the full-length isoform, rather than the total transcript level, that correlates with enzymatic activity. Telomerase reactivation may be critical in the early stages of tumorigenesis, when progressive telomere shortening may be limiting cell viability. The aim of this study was to investigate the relationship between telomere length and hTERT splice variant expression patterns in benign and well-differentiated malignant thyroid tumors. Telomere lengths of 61 thyroid tumors were examined by fluorescence in situ hybridization, comparing tumors with adjacent normal thyroid tissue on the same slide. Expression patterns of hTERT splice variants were evaluated by quantitative and nested RT-PCR. Telomere length was inversely correlated with percentage of full-length hTERT expression rather than with total hTERT expression levels. Short telomeres and high fractions of full-length hTERT transcripts were associated with follicular and papillary thyroid carcinomas, whereas long telomeres and low levels of full-length hTERT were associated with benign thyroid nodules. Intermediate levels of full-length hTERT and telomere length were found in follicular variant of papillary thyroid carcinomas and follicular adenomas.

Authors+Show Affiliations

Department of Surgery, The Johns Hopkins Medical Institutions, Baltimore, Maryland, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21763260

Citation

Wang, Yongchun, et al. "Telomere Length Is Related to Alternative Splice Patterns of Telomerase in Thyroid Tumors." The American Journal of Pathology, vol. 179, no. 3, 2011, pp. 1415-24.
Wang Y, Meeker AK, Kowalski J, et al. Telomere length is related to alternative splice patterns of telomerase in thyroid tumors. Am J Pathol. 2011;179(3):1415-24.
Wang, Y., Meeker, A. K., Kowalski, J., Tsai, H. L., Somervell, H., Heaphy, C., Sangenario, L. E., Prasad, N., Westra, W. H., Zeiger, M. A., & Umbricht, C. B. (2011). Telomere length is related to alternative splice patterns of telomerase in thyroid tumors. The American Journal of Pathology, 179(3), 1415-24. https://doi.org/10.1016/j.ajpath.2011.05.056
Wang Y, et al. Telomere Length Is Related to Alternative Splice Patterns of Telomerase in Thyroid Tumors. Am J Pathol. 2011;179(3):1415-24. PubMed PMID: 21763260.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Telomere length is related to alternative splice patterns of telomerase in thyroid tumors. AU - Wang,Yongchun, AU - Meeker,Alan K, AU - Kowalski,Jeanne, AU - Tsai,Hua-Ling, AU - Somervell,Helina, AU - Heaphy,Christopher, AU - Sangenario,Lauren E, AU - Prasad,Nijaguna, AU - Westra,William H, AU - Zeiger,Martha A, AU - Umbricht,Christopher B, Y1 - 2011/07/16/ PY - 2011/01/21/received PY - 2011/04/19/revised PY - 2011/05/11/accepted PY - 2011/7/19/entrez PY - 2011/7/19/pubmed PY - 2011/12/14/medline SP - 1415 EP - 24 JF - The American journal of pathology JO - Am J Pathol VL - 179 IS - 3 N2 - Telomere dysfunction and aberrant telomerase expression play important roles in tumorigenesis. In thyroid tumors, three possibly inhibitory splice variants of the active full-length isoform of human telomerase reverse transcriptase (hTERT) may be expressed. These variants might regulate telomerase activity and telomere length because it is the fraction of the full-length isoform, rather than the total transcript level, that correlates with enzymatic activity. Telomerase reactivation may be critical in the early stages of tumorigenesis, when progressive telomere shortening may be limiting cell viability. The aim of this study was to investigate the relationship between telomere length and hTERT splice variant expression patterns in benign and well-differentiated malignant thyroid tumors. Telomere lengths of 61 thyroid tumors were examined by fluorescence in situ hybridization, comparing tumors with adjacent normal thyroid tissue on the same slide. Expression patterns of hTERT splice variants were evaluated by quantitative and nested RT-PCR. Telomere length was inversely correlated with percentage of full-length hTERT expression rather than with total hTERT expression levels. Short telomeres and high fractions of full-length hTERT transcripts were associated with follicular and papillary thyroid carcinomas, whereas long telomeres and low levels of full-length hTERT were associated with benign thyroid nodules. Intermediate levels of full-length hTERT and telomere length were found in follicular variant of papillary thyroid carcinomas and follicular adenomas. SN - 1525-2191 UR - https://www.unboundmedicine.com/medline/citation/21763260/Telomere_length_is_related_to_alternative_splice_patterns_of_telomerase_in_thyroid_tumors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002-9440(11)00556-6 DB - PRIME DP - Unbound Medicine ER -