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Dengue virus seroprevalence among febrile patients in Bamako, Mali: results of a 2006 surveillance study.
Vector Borne Zoonotic Dis. 2011 Nov; 11(11):1479-85.VB

Abstract

BACKGROUND

Dengue viruses (DENV) are endemic in over 100 countries worldwide, and annually 50 to 100 million people are infected by one of the four DENV serotypes, whereas over 2.5 billion people are at risk for infection. West African countries lack the surveillance to determine the true incidence of dengue; hence, this disease is likely significantly underestimated. In Mali, ?14 million people are potentially at risk of acquiring a dengue infection.

METHODS AND FINDINGS

A serosurvey for DENV was conducted on 95 human serum samples obtained from the Institute National de Recherche en Sante Publique in 2006. DENV-specific IgM and IgG enzyme-linked immunosorbent assays were performed on all samples, and a subset was tested using the plaque-reduction neutralization test against the DENV and yellow fever virus (YFV). Samples collected during the acute infection (0-5 days postonset of symptoms) were tested for dengue NS1 antigen and reverse-transcriptase polymerase chain reaction for Flaviviruses, Alphaviruses, and Bunyaviruses RNA. A total of 87 (93%) of samples were positive for anti-DENV IgG antibodies. Of a subset of 13 IgG positive samples, 2 samples neutralized monotypically against DENV-1 and -2, whereas 3 others neutralized broadly against YFV and multiple DENV. Although no polymerase chain reaction positives were found, DENV NS1 was detected in 1 of the 20 acute samples tested.

CONCLUSIONS

Of the 93 human serum samples tested, the dengue prevalence based on dengue IgG enzyme-linked immunosorbent assay results was 93%. Three DENV specific positive samples and two YFV positives were identified by plaque-reduction neutralization test. Finally, one sample tested positive for dengue NS1, thus suggestive of an acute infection within 14 days of obtaining the sample from the patient. Based on these serological data from this study, YFV and DENV appear to be co-circulating in Mali.

Authors+Show Affiliations

Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Dengue Branch, San Juan, Puerto Rico.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21767159

Citation

Phoutrides, Elena K., et al. "Dengue Virus Seroprevalence Among Febrile Patients in Bamako, Mali: Results of a 2006 Surveillance Study." Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), vol. 11, no. 11, 2011, pp. 1479-85.
Phoutrides EK, Coulibaly MB, George CM, et al. Dengue virus seroprevalence among febrile patients in Bamako, Mali: results of a 2006 surveillance study. Vector Borne Zoonotic Dis. 2011;11(11):1479-85.
Phoutrides, E. K., Coulibaly, M. B., George, C. M., Sacko, A., Traore, S., Bessoff, K., Wiley, M. R., Kolivras, K. N., Adelman, Z., Traore, M., & Hunsperger, E. A. (2011). Dengue virus seroprevalence among febrile patients in Bamako, Mali: results of a 2006 surveillance study. Vector Borne and Zoonotic Diseases (Larchmont, N.Y.), 11(11), 1479-85. https://doi.org/10.1089/vbz.2011.0622
Phoutrides EK, et al. Dengue Virus Seroprevalence Among Febrile Patients in Bamako, Mali: Results of a 2006 Surveillance Study. Vector Borne Zoonotic Dis. 2011;11(11):1479-85. PubMed PMID: 21767159.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dengue virus seroprevalence among febrile patients in Bamako, Mali: results of a 2006 surveillance study. AU - Phoutrides,Elena K, AU - Coulibaly,Mamadou B, AU - George,Christine M, AU - Sacko,Adama, AU - Traore,Sekou, AU - Bessoff,Kovi, AU - Wiley,Michael R, AU - Kolivras,Korine N, AU - Adelman,Zach, AU - Traore,Mohamed, AU - Hunsperger,Elizabeth A, Y1 - 2011/07/18/ PY - 2011/7/20/entrez PY - 2011/7/20/pubmed PY - 2012/5/2/medline SP - 1479 EP - 85 JF - Vector borne and zoonotic diseases (Larchmont, N.Y.) JO - Vector Borne Zoonotic Dis VL - 11 IS - 11 N2 - BACKGROUND: Dengue viruses (DENV) are endemic in over 100 countries worldwide, and annually 50 to 100 million people are infected by one of the four DENV serotypes, whereas over 2.5 billion people are at risk for infection. West African countries lack the surveillance to determine the true incidence of dengue; hence, this disease is likely significantly underestimated. In Mali, ?14 million people are potentially at risk of acquiring a dengue infection. METHODS AND FINDINGS: A serosurvey for DENV was conducted on 95 human serum samples obtained from the Institute National de Recherche en Sante Publique in 2006. DENV-specific IgM and IgG enzyme-linked immunosorbent assays were performed on all samples, and a subset was tested using the plaque-reduction neutralization test against the DENV and yellow fever virus (YFV). Samples collected during the acute infection (0-5 days postonset of symptoms) were tested for dengue NS1 antigen and reverse-transcriptase polymerase chain reaction for Flaviviruses, Alphaviruses, and Bunyaviruses RNA. A total of 87 (93%) of samples were positive for anti-DENV IgG antibodies. Of a subset of 13 IgG positive samples, 2 samples neutralized monotypically against DENV-1 and -2, whereas 3 others neutralized broadly against YFV and multiple DENV. Although no polymerase chain reaction positives were found, DENV NS1 was detected in 1 of the 20 acute samples tested. CONCLUSIONS: Of the 93 human serum samples tested, the dengue prevalence based on dengue IgG enzyme-linked immunosorbent assay results was 93%. Three DENV specific positive samples and two YFV positives were identified by plaque-reduction neutralization test. Finally, one sample tested positive for dengue NS1, thus suggestive of an acute infection within 14 days of obtaining the sample from the patient. Based on these serological data from this study, YFV and DENV appear to be co-circulating in Mali. SN - 1557-7759 UR - https://www.unboundmedicine.com/medline/citation/21767159/Dengue_virus_seroprevalence_among_febrile_patients_in_Bamako_Mali:_results_of_a_2006_surveillance_study_ DB - PRIME DP - Unbound Medicine ER -