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Anti-enteric bacterial activity and phytochemical analysis of the seed kernel extract of Mangifera indica Linnaeus against Shigella dysenteriae (Shiga, corrig.) Castellani and Chalmers.
Asian Pac J Trop Med. 2011 Apr; 4(4):294-300.AP

Abstract

OBJECTIVE

To evaluate the phytochemical and anti-bacterial efficacy of the seed kernel extract of Mangifera indica (M. indica) against the enteropathogen, Shigella dysenteriae (S. dysenteriae), isolated from the diarrhoeal stool specimens.

METHODS

The preliminary phytochemical screening was performed by the standard methods as described by Harborne. Cold extraction method was employed to extract the bioactive compounds from mango seed kernel. Disc diffusion method was adopted to screen antibacterial activity. Minimum inhibitory concentration (MIC) was evaluated by agar dilution method. The crude extracts were partially purified by thin layer chromatography (TLC) and the fractions were analyzed by high performance thin layer chromatography (HPTLC) to identify the bioactive compounds.

RESULTS

Phytochemical scrutiny of M. indica indicated the presence of phytochemical constituents such as alkaloids, gums, flavanoids, phenols, saponins, steroids, tannins and xanthoproteins. Antibacterial activity was observed in two crude extracts and various fractions viz. hexane, benzene, chloroform, methanol and water. MIC of methanol fraction was found to be (95±11.8) μg/mL. MIC of other fractions ranged from 130-380 μg/mL.

CONCLUSIONS

The present study confirmed that each crude extracts and fractions of M. indica have significant antimicrobial activity against the isolated pathogen S. dysenteriae. The antibacterial activity may be due to the phytochemical constituents of the mango seed kernel. The phytochemical tannin could be the reason for its antibacterial activity.

Authors+Show Affiliations

Department of Microbiology, Srimad Andavan Arts and Science College, Tiruchirapalli-620 005, Tamilnadu, India.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21771473

Citation

Rajan, S, et al. "Anti-enteric Bacterial Activity and Phytochemical Analysis of the Seed Kernel Extract of Mangifera Indica Linnaeus Against Shigella Dysenteriae (Shiga, Corrig.) Castellani and Chalmers." Asian Pacific Journal of Tropical Medicine, vol. 4, no. 4, 2011, pp. 294-300.
Rajan S, Thirunalasundari T, Jeeva S. Anti-enteric bacterial activity and phytochemical analysis of the seed kernel extract of Mangifera indica Linnaeus against Shigella dysenteriae (Shiga, corrig.) Castellani and Chalmers. Asian Pac J Trop Med. 2011;4(4):294-300.
Rajan, S., Thirunalasundari, T., & Jeeva, S. (2011). Anti-enteric bacterial activity and phytochemical analysis of the seed kernel extract of Mangifera indica Linnaeus against Shigella dysenteriae (Shiga, corrig.) Castellani and Chalmers. Asian Pacific Journal of Tropical Medicine, 4(4), 294-300. https://doi.org/10.1016/S1995-7645(11)60089-8
Rajan S, Thirunalasundari T, Jeeva S. Anti-enteric Bacterial Activity and Phytochemical Analysis of the Seed Kernel Extract of Mangifera Indica Linnaeus Against Shigella Dysenteriae (Shiga, Corrig.) Castellani and Chalmers. Asian Pac J Trop Med. 2011;4(4):294-300. PubMed PMID: 21771473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-enteric bacterial activity and phytochemical analysis of the seed kernel extract of Mangifera indica Linnaeus against Shigella dysenteriae (Shiga, corrig.) Castellani and Chalmers. AU - Rajan,S, AU - Thirunalasundari,T, AU - Jeeva,S, Y1 - 2011/05/29/ PY - 2011/01/07/received PY - 2011/02/01/revised PY - 2011/03/15/accepted PY - 2011/7/21/entrez PY - 2011/7/21/pubmed PY - 2012/1/24/medline SP - 294 EP - 300 JF - Asian Pacific journal of tropical medicine JO - Asian Pac J Trop Med VL - 4 IS - 4 N2 - OBJECTIVE: To evaluate the phytochemical and anti-bacterial efficacy of the seed kernel extract of Mangifera indica (M. indica) against the enteropathogen, Shigella dysenteriae (S. dysenteriae), isolated from the diarrhoeal stool specimens. METHODS: The preliminary phytochemical screening was performed by the standard methods as described by Harborne. Cold extraction method was employed to extract the bioactive compounds from mango seed kernel. Disc diffusion method was adopted to screen antibacterial activity. Minimum inhibitory concentration (MIC) was evaluated by agar dilution method. The crude extracts were partially purified by thin layer chromatography (TLC) and the fractions were analyzed by high performance thin layer chromatography (HPTLC) to identify the bioactive compounds. RESULTS: Phytochemical scrutiny of M. indica indicated the presence of phytochemical constituents such as alkaloids, gums, flavanoids, phenols, saponins, steroids, tannins and xanthoproteins. Antibacterial activity was observed in two crude extracts and various fractions viz. hexane, benzene, chloroform, methanol and water. MIC of methanol fraction was found to be (95±11.8) μg/mL. MIC of other fractions ranged from 130-380 μg/mL. CONCLUSIONS: The present study confirmed that each crude extracts and fractions of M. indica have significant antimicrobial activity against the isolated pathogen S. dysenteriae. The antibacterial activity may be due to the phytochemical constituents of the mango seed kernel. The phytochemical tannin could be the reason for its antibacterial activity. SN - 2352-4146 UR - https://www.unboundmedicine.com/medline/citation/21771473/Anti_enteric_bacterial_activity_and_phytochemical_analysis_of_the_seed_kernel_extract_of_Mangifera_indica_Linnaeus_against_Shigella_dysenteriae__Shiga_corrig___Castellani_and_Chalmers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1995-7645(11)60089-8 DB - PRIME DP - Unbound Medicine ER -