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Structural elucidation of a heteroglycan from the fruiting bodies of Agaricus blazei Murill.
Int J Biol Macromol. 2011 Nov 01; 49(4):716-20.IJ

Abstract

One water-soluble polysaccharide (ABP-W1) was purified from the fruiting bodies of Agaricus blazei by DEAE Sepharose Fast Flow and Sepharose 6 Fast Flow column chromatography. Its molecular weight was about 3.9×10(2) kDa as determined by high-performance size-exclusion chromatography (HPSEC). The structural feature of ABP-W1 was investigated by a combination of chemical and instrumental analysis, including partial hydrolysis with acid, periodate oxidation-Smith degradation, acetylation, methylation analysis and nuclear magnetic resonance spectroscopy (NMR (1)H, (13)C). The results revealed that ABP-W1 had a backbone consisting of (1→6)-linked-α-D-galactopyranosyl and (1→2,6)-linked-α-D-glucopyranosyl, which was branched with one single terminal (1→)-α-D-glucopyranosyl at the O-2 position of (1→2,6)-linked-α-D-glucopyranosyl along the main chain in the ratio of 1:1:1. The observation of the complex-formation between ABP-W1 and Congo Red indicated that ABP-W1 probably existed in a triple-strand helical conformation in water. Based on the data obtained, ABP-W1 was composed of a repeating unit with a structure as below: [structure: see text].

Authors+Show Affiliations

Qiqihar Medical University, Qiqihar, PR China. jichengliu@yahoo.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21771611

Citation

Liu, Jicheng, et al. "Structural Elucidation of a Heteroglycan From the Fruiting Bodies of Agaricus Blazei Murill." International Journal of Biological Macromolecules, vol. 49, no. 4, 2011, pp. 716-20.
Liu J, Zhang C, Wang Y, et al. Structural elucidation of a heteroglycan from the fruiting bodies of Agaricus blazei Murill. Int J Biol Macromol. 2011;49(4):716-20.
Liu, J., Zhang, C., Wang, Y., Yu, H., Liu, H., Wang, L., Yang, X., Liu, Z., Wen, X., Sun, Y., Yu, C., & Liu, L. (2011). Structural elucidation of a heteroglycan from the fruiting bodies of Agaricus blazei Murill. International Journal of Biological Macromolecules, 49(4), 716-20. https://doi.org/10.1016/j.ijbiomac.2011.07.003
Liu J, et al. Structural Elucidation of a Heteroglycan From the Fruiting Bodies of Agaricus Blazei Murill. Int J Biol Macromol. 2011 Nov 1;49(4):716-20. PubMed PMID: 21771611.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural elucidation of a heteroglycan from the fruiting bodies of Agaricus blazei Murill. AU - Liu,Jicheng, AU - Zhang,Chunjing, AU - Wang,Yajun, AU - Yu,Haitao, AU - Liu,Han, AU - Wang,Liping, AU - Yang,Xiuzhen, AU - Liu,Zhecheng, AU - Wen,Xianchun, AU - Sun,Yongxu, AU - Yu,Chunlei, AU - Liu,Lei, Y1 - 2011/07/13/ PY - 2011/05/25/received PY - 2011/06/26/revised PY - 2011/07/04/accepted PY - 2011/7/21/entrez PY - 2011/7/21/pubmed PY - 2011/12/31/medline SP - 716 EP - 20 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 49 IS - 4 N2 - One water-soluble polysaccharide (ABP-W1) was purified from the fruiting bodies of Agaricus blazei by DEAE Sepharose Fast Flow and Sepharose 6 Fast Flow column chromatography. Its molecular weight was about 3.9×10(2) kDa as determined by high-performance size-exclusion chromatography (HPSEC). The structural feature of ABP-W1 was investigated by a combination of chemical and instrumental analysis, including partial hydrolysis with acid, periodate oxidation-Smith degradation, acetylation, methylation analysis and nuclear magnetic resonance spectroscopy (NMR (1)H, (13)C). The results revealed that ABP-W1 had a backbone consisting of (1→6)-linked-α-D-galactopyranosyl and (1→2,6)-linked-α-D-glucopyranosyl, which was branched with one single terminal (1→)-α-D-glucopyranosyl at the O-2 position of (1→2,6)-linked-α-D-glucopyranosyl along the main chain in the ratio of 1:1:1. The observation of the complex-formation between ABP-W1 and Congo Red indicated that ABP-W1 probably existed in a triple-strand helical conformation in water. Based on the data obtained, ABP-W1 was composed of a repeating unit with a structure as below: [structure: see text]. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/21771611/Structural_elucidation_of_a_heteroglycan_from_the_fruiting_bodies_of_Agaricus_blazei_Murill_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(11)00281-9 DB - PRIME DP - Unbound Medicine ER -