TY - JOUR T1 - Structural elucidation of a heteroglycan from the fruiting bodies of Agaricus blazei Murill. AU - Liu,Jicheng, AU - Zhang,Chunjing, AU - Wang,Yajun, AU - Yu,Haitao, AU - Liu,Han, AU - Wang,Liping, AU - Yang,Xiuzhen, AU - Liu,Zhecheng, AU - Wen,Xianchun, AU - Sun,Yongxu, AU - Yu,Chunlei, AU - Liu,Lei, Y1 - 2011/07/13/ PY - 2011/05/25/received PY - 2011/06/26/revised PY - 2011/07/04/accepted PY - 2011/7/21/entrez PY - 2011/7/21/pubmed PY - 2011/12/31/medline SP - 716 EP - 20 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 49 IS - 4 N2 - One water-soluble polysaccharide (ABP-W1) was purified from the fruiting bodies of Agaricus blazei by DEAE Sepharose Fast Flow and Sepharose 6 Fast Flow column chromatography. Its molecular weight was about 3.9×10(2) kDa as determined by high-performance size-exclusion chromatography (HPSEC). The structural feature of ABP-W1 was investigated by a combination of chemical and instrumental analysis, including partial hydrolysis with acid, periodate oxidation-Smith degradation, acetylation, methylation analysis and nuclear magnetic resonance spectroscopy (NMR (1)H, (13)C). The results revealed that ABP-W1 had a backbone consisting of (1→6)-linked-α-D-galactopyranosyl and (1→2,6)-linked-α-D-glucopyranosyl, which was branched with one single terminal (1→)-α-D-glucopyranosyl at the O-2 position of (1→2,6)-linked-α-D-glucopyranosyl along the main chain in the ratio of 1:1:1. The observation of the complex-formation between ABP-W1 and Congo Red indicated that ABP-W1 probably existed in a triple-strand helical conformation in water. Based on the data obtained, ABP-W1 was composed of a repeating unit with a structure as below: [structure: see text]. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/21771611/Structural_elucidation_of_a_heteroglycan_from_the_fruiting_bodies_of_Agaricus_blazei_Murill_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(11)00281-9 DB - PRIME DP - Unbound Medicine ER -