Tags

Type your tag names separated by a space and hit enter

Antioxidant activity and phytochemical screening of the methanol extracts of Euphorbia hirta L.
Asian Pac J Trop Med. 2011 May; 4(5):386-90.AP

Abstract

OBJECTIVE

To assess antioxidant activities of different parts of Euphorbia hirta (E. hirta), and to search for new sources of safe and inexpensive antioxidants.

METHODS

Samples of leaves, stems, flowers and roots from E. hirta were tested for total phenolic content, and flavonoids content and in vitro antioxidant activity by diphenyl-1-picrylhydrazyl (DPPH) assay and reducing power was measured using cyanoferrate method.

RESULTS

The leaves extract exhibited a maximum DPPH scavenging activity of (72.96±0.78)% followed by the flowers, roots and stems whose scavenging activities were (52.45±0.66)%, (48.59±0.97)%, and (44.42±0.94)%, respectively. The standard butylated hydroxytoluene (BHT) was (75.13±0.75)%. The IC(50) for leaves, flowers, roots, stems and BHT were 0.803, 0.972, 0.989, 1.358 and 0.794 mg/mL, respectively. The reducing power of the leaves extract was comparable with that of ascorbic acid and found to be dose dependent. Leaves extract had the highest total phenolic content [(206.17±1.95) mg GAE/g], followed by flowers, roots and stems extracts which were (117.08±3.10) mg GAE/g, (83.15±1.19) mg GAE/g, and (65.70±1.72) mg GAE/g, respectively. On the other hand, total flavonoids content also from leave had the highest value [(37.970±0.003) mg CEQ/g], followed by flowers, roots and stems extracts which were (35.200±0.002) mg CEQ/g, (24.350±0.006) mg CEQ/g, and (24.120±0.004) mg CEQ/g, respectively. HPTLC bioautography analysis of phenolic and antioxidant substance revealed phenolic compounds. Phytochemical screening of E. hirta leaf extract revealed the presence of reducing sugars, terpenoids, alkaloids, steroids, tannins, flavanoids and phenolic compounds.

CONCLUSIONS

These results suggeste that E. hirta have strong antioxidant potential. Further study is necessary for isolation and characterization of the active antioxidant agents, which can be used to treat various oxidative stress-related diseases.

Authors+Show Affiliations

Biology Division, School of Distance Education, Universiti Sains Malaysia, USM 11800, Pulau Pinang, Malaysia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21771682

Citation

Basma, Abu Arra, et al. "Antioxidant Activity and Phytochemical Screening of the Methanol Extracts of Euphorbia Hirta L." Asian Pacific Journal of Tropical Medicine, vol. 4, no. 5, 2011, pp. 386-90.
Basma AA, Zakaria Z, Latha LY, et al. Antioxidant activity and phytochemical screening of the methanol extracts of Euphorbia hirta L. Asian Pac J Trop Med. 2011;4(5):386-90.
Basma, A. A., Zakaria, Z., Latha, L. Y., & Sasidharan, S. (2011). Antioxidant activity and phytochemical screening of the methanol extracts of Euphorbia hirta L. Asian Pacific Journal of Tropical Medicine, 4(5), 386-90. https://doi.org/10.1016/S1995-7645(11)60109-0
Basma AA, et al. Antioxidant Activity and Phytochemical Screening of the Methanol Extracts of Euphorbia Hirta L. Asian Pac J Trop Med. 2011;4(5):386-90. PubMed PMID: 21771682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity and phytochemical screening of the methanol extracts of Euphorbia hirta L. AU - Basma,Abu Arra, AU - Zakaria,Zuraini, AU - Latha,Lacimanan Yoga, AU - Sasidharan,Sreenivasan, Y1 - 2011/06/22/ PY - 2011/02/25/received PY - 2011/03/27/revised PY - 2011/04/02/accepted PY - 2011/7/21/entrez PY - 2011/7/21/pubmed PY - 2012/2/1/medline SP - 386 EP - 90 JF - Asian Pacific journal of tropical medicine JO - Asian Pac J Trop Med VL - 4 IS - 5 N2 - OBJECTIVE: To assess antioxidant activities of different parts of Euphorbia hirta (E. hirta), and to search for new sources of safe and inexpensive antioxidants. METHODS: Samples of leaves, stems, flowers and roots from E. hirta were tested for total phenolic content, and flavonoids content and in vitro antioxidant activity by diphenyl-1-picrylhydrazyl (DPPH) assay and reducing power was measured using cyanoferrate method. RESULTS: The leaves extract exhibited a maximum DPPH scavenging activity of (72.96±0.78)% followed by the flowers, roots and stems whose scavenging activities were (52.45±0.66)%, (48.59±0.97)%, and (44.42±0.94)%, respectively. The standard butylated hydroxytoluene (BHT) was (75.13±0.75)%. The IC(50) for leaves, flowers, roots, stems and BHT were 0.803, 0.972, 0.989, 1.358 and 0.794 mg/mL, respectively. The reducing power of the leaves extract was comparable with that of ascorbic acid and found to be dose dependent. Leaves extract had the highest total phenolic content [(206.17±1.95) mg GAE/g], followed by flowers, roots and stems extracts which were (117.08±3.10) mg GAE/g, (83.15±1.19) mg GAE/g, and (65.70±1.72) mg GAE/g, respectively. On the other hand, total flavonoids content also from leave had the highest value [(37.970±0.003) mg CEQ/g], followed by flowers, roots and stems extracts which were (35.200±0.002) mg CEQ/g, (24.350±0.006) mg CEQ/g, and (24.120±0.004) mg CEQ/g, respectively. HPTLC bioautography analysis of phenolic and antioxidant substance revealed phenolic compounds. Phytochemical screening of E. hirta leaf extract revealed the presence of reducing sugars, terpenoids, alkaloids, steroids, tannins, flavanoids and phenolic compounds. CONCLUSIONS: These results suggeste that E. hirta have strong antioxidant potential. Further study is necessary for isolation and characterization of the active antioxidant agents, which can be used to treat various oxidative stress-related diseases. SN - 2352-4146 UR - https://www.unboundmedicine.com/medline/citation/21771682/Antioxidant_activity_and_phytochemical_screening_of_the_methanol_extracts_of_Euphorbia_hirta_L_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1995-7645(11)60109-0 DB - PRIME DP - Unbound Medicine ER -