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Engineering of 2-Cys peroxiredoxin for enhanced stress-tolerance.
Mol Cells. 2011 Sep; 32(3):257-64.MC

Abstract

A typical 2-cysteine peroxiredoxin (2-Cys Prx)-like protein (PpPrx) that alternatively acts as a peroxidase or a molecular chaperone in Pseudomonas putida KT2440 was previously characterized. The dual functions of PpPrx are regulated by the existence of an additional Cys(112) between the active Cys(51) and Cys(171) residues. In the present study, additional Cys residues (Cys(31), Cys(112), and Cys(192)) were added to PpPrx variants to improve their enzymatic function. The optimal position of the additional Cys residues for the dual functionality was assessed. The peroxidase activities of the S31C and Y192C mutants were increased 3- to 4-fold compared to the wild-type, while the chaperone activity was maintained at > 66% of PpPrx. To investigate whether optimization of the dual functions could enhance stress-tolerance in vivo, a complementation study was performed. The S31C and Y192C mutants showed a much greater tolerance than other variants under a complex condition of heat and oxidative stresses. The optimized dual functions of PpPrx could be adapted for use in bioengineering systems and industries, such as to develop organisms that are more resistant to extreme environments.

Authors+Show Affiliations

Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21773675

Citation

An, Byung Chull, et al. "Engineering of 2-Cys Peroxiredoxin for Enhanced Stress-tolerance." Molecules and Cells, vol. 32, no. 3, 2011, pp. 257-64.
An BC, Lee SS, Lee JT, et al. Engineering of 2-Cys peroxiredoxin for enhanced stress-tolerance. Mol Cells. 2011;32(3):257-64.
An, B. C., Lee, S. S., Lee, J. T., Hong, S. H., Wi, S. G., & Chung, B. Y. (2011). Engineering of 2-Cys peroxiredoxin for enhanced stress-tolerance. Molecules and Cells, 32(3), 257-64. https://doi.org/10.1007/s10059-011-1047-x
An BC, et al. Engineering of 2-Cys Peroxiredoxin for Enhanced Stress-tolerance. Mol Cells. 2011;32(3):257-64. PubMed PMID: 21773675.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Engineering of 2-Cys peroxiredoxin for enhanced stress-tolerance. AU - An,Byung Chull, AU - Lee,Seung Sik, AU - Lee,Jae Taek, AU - Hong,Sung Hyun, AU - Wi,Seung Gon, AU - Chung,Byung Yeoup, Y1 - 2011/07/15/ PY - 2011/03/11/received PY - 2011/06/13/accepted PY - 2011/06/13/revised PY - 2011/7/21/entrez PY - 2011/7/21/pubmed PY - 2012/1/31/medline SP - 257 EP - 64 JF - Molecules and cells JO - Mol Cells VL - 32 IS - 3 N2 - A typical 2-cysteine peroxiredoxin (2-Cys Prx)-like protein (PpPrx) that alternatively acts as a peroxidase or a molecular chaperone in Pseudomonas putida KT2440 was previously characterized. The dual functions of PpPrx are regulated by the existence of an additional Cys(112) between the active Cys(51) and Cys(171) residues. In the present study, additional Cys residues (Cys(31), Cys(112), and Cys(192)) were added to PpPrx variants to improve their enzymatic function. The optimal position of the additional Cys residues for the dual functionality was assessed. The peroxidase activities of the S31C and Y192C mutants were increased 3- to 4-fold compared to the wild-type, while the chaperone activity was maintained at > 66% of PpPrx. To investigate whether optimization of the dual functions could enhance stress-tolerance in vivo, a complementation study was performed. The S31C and Y192C mutants showed a much greater tolerance than other variants under a complex condition of heat and oxidative stresses. The optimized dual functions of PpPrx could be adapted for use in bioengineering systems and industries, such as to develop organisms that are more resistant to extreme environments. SN - 0219-1032 UR - https://www.unboundmedicine.com/medline/citation/21773675/Engineering_of_2_Cys_peroxiredoxin_for_enhanced_stress_tolerance_ L2 - http://www.molcells.org/journal/view.html?year=2011&volume=32&number=3&spage=257 DB - PRIME DP - Unbound Medicine ER -