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Inhibition of angiotensin converting enzyme (ACE) activity by polyphenols from tea (Camellia sinensis) and links to processing method.
Food Funct. 2011 Jun; 2(6):310-9.FF

Abstract

The focus of this study was to investigate Angiotensin Converting Enzyme (ACE) inhibiting activity across 34 teas (Camellia sinensis) produced by 5 different processing methods including green (GT), oolong (OT), white (WT), black (BT) and dark (DT) teas. In vitro ACE inhibitory activity was affected by the tea processing method with IC(50) values for ACE inhibition: green < oolong < white < black < dark teas. Substrate-dependence of the reaction kinetics was studied for GT and BT polyphenolic size fractions either < or > 3 kDa and also Green Tea Polyphenolic Isolate (GTPI), and revealed that enzyme velocity curves fitted allosteric, not Michaelis-Menten, relationships. Inhibition was weakly dependent on substrate concentration for GT fraction >3 kDa and independent of substrate concentration for all other GT and BT size fractions and GTPI. Furthermore, evidence for direct inactivation of ACE by GTPI was demonstrated. Overall, the results suggest that tea polyphenolics exert a mixed mode of in vitro inhibition of ACE, mostly of a kinetically uncompetitive type. The results are discussed in the context of in vivo and epidemiological evidence for regulation of blood pressure by tea consumption.

Authors+Show Affiliations

CSIRO Preventative Health Flagship, CSIRO Food and Nutritional Sciences, 671 Sneydes Road, Private Bag 16, Werribee, Victoria 3030, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21779569

Citation

Dong, Junjie, et al. "Inhibition of Angiotensin Converting Enzyme (ACE) Activity By Polyphenols From Tea (Camellia Sinensis) and Links to Processing Method." Food & Function, vol. 2, no. 6, 2011, pp. 310-9.
Dong J, Xu X, Liang Y, et al. Inhibition of angiotensin converting enzyme (ACE) activity by polyphenols from tea (Camellia sinensis) and links to processing method. Food Funct. 2011;2(6):310-9.
Dong, J., Xu, X., Liang, Y., Head, R., & Bennett, L. (2011). Inhibition of angiotensin converting enzyme (ACE) activity by polyphenols from tea (Camellia sinensis) and links to processing method. Food & Function, 2(6), 310-9. https://doi.org/10.1039/c1fo10023h
Dong J, et al. Inhibition of Angiotensin Converting Enzyme (ACE) Activity By Polyphenols From Tea (Camellia Sinensis) and Links to Processing Method. Food Funct. 2011;2(6):310-9. PubMed PMID: 21779569.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of angiotensin converting enzyme (ACE) activity by polyphenols from tea (Camellia sinensis) and links to processing method. AU - Dong,Junjie, AU - Xu,Xinqing, AU - Liang,Yuerong, AU - Head,Richard, AU - Bennett,Louise, Y1 - 2011/05/26/ PY - 2011/7/23/entrez PY - 2011/7/23/pubmed PY - 2012/1/25/medline SP - 310 EP - 9 JF - Food & function JO - Food Funct VL - 2 IS - 6 N2 - The focus of this study was to investigate Angiotensin Converting Enzyme (ACE) inhibiting activity across 34 teas (Camellia sinensis) produced by 5 different processing methods including green (GT), oolong (OT), white (WT), black (BT) and dark (DT) teas. In vitro ACE inhibitory activity was affected by the tea processing method with IC(50) values for ACE inhibition: green < oolong < white < black < dark teas. Substrate-dependence of the reaction kinetics was studied for GT and BT polyphenolic size fractions either < or > 3 kDa and also Green Tea Polyphenolic Isolate (GTPI), and revealed that enzyme velocity curves fitted allosteric, not Michaelis-Menten, relationships. Inhibition was weakly dependent on substrate concentration for GT fraction >3 kDa and independent of substrate concentration for all other GT and BT size fractions and GTPI. Furthermore, evidence for direct inactivation of ACE by GTPI was demonstrated. Overall, the results suggest that tea polyphenolics exert a mixed mode of in vitro inhibition of ACE, mostly of a kinetically uncompetitive type. The results are discussed in the context of in vivo and epidemiological evidence for regulation of blood pressure by tea consumption. SN - 2042-650X UR - https://www.unboundmedicine.com/medline/citation/21779569/Inhibition_of_angiotensin_converting_enzyme__ACE__activity_by_polyphenols_from_tea__Camellia_sinensis__and_links_to_processing_method_ L2 - https://doi.org/10.1039/c1fo10023h DB - PRIME DP - Unbound Medicine ER -