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The mechanoreceptor TRPV4 is localized in adherence junctions of the human bladder urothelium: a morphological study.
J Urol. 2011 Sep; 186(3):1121-7.JU

Abstract

PURPOSE

TRPV4 (transient receptor potential vanilloid 4 channel) is a nonselective cation channel involved in different sensory functions that was recently implicated in bladder mechanosensation. We investigated the cellular site of TRPV4 in bladder urothelium and explored a molecular connection between TRPV4 and urothelial adherence junctions.

MATERIALS AND METHODS

We obtained healthy tissues sections from cystectomy in humans due to cancer in 3 and noncancerous conditions in 2. Besides human biopsies tissues from 7 normal and 7 TRPV4-/-mice, and the urothelial cell line RT4 were also used. Experiments were done with polyclonal antibody against TRPV4 (against the N-terminus of rat TRPV4). A molecular connection between TRPV4 and different adherence junction components was investigated using immunofluorescence, Western blot and immunoprecipitation.

RESULTS

Results revealed TRPV4 on urothelial cell membranes near adherence junctions. Results were comparable in the urothelial cell line, human bladders and mouse bladders. Subsequent immunoprecipitation experiments established a molecular connection of TRPV4 to α-catenin, an integral part of the adherence junction that catenates E-cadherin to the actin-microfilament network.

CONCLUSIONS

Results provide evidence for the location of TRPV4 in human bladder urothelium. TRPV4 is molecularly connected to adherence junctions on the urothelial cell membrane. TRPV4 coupling to a rigid intracellular and intercellular structural network would agree with the hypothesis that TRPV4 can be activated by bladder stretch.

Authors+Show Affiliations

Department of Urology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands. d.janssen@uro.umcn.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21784462

Citation

Janssen, Dick A W., et al. "The Mechanoreceptor TRPV4 Is Localized in Adherence Junctions of the Human Bladder Urothelium: a Morphological Study." The Journal of Urology, vol. 186, no. 3, 2011, pp. 1121-7.
Janssen DA, Hoenderop JG, Jansen KC, et al. The mechanoreceptor TRPV4 is localized in adherence junctions of the human bladder urothelium: a morphological study. J Urol. 2011;186(3):1121-7.
Janssen, D. A., Hoenderop, J. G., Jansen, K. C., Kemp, A. W., Heesakkers, J. P., & Schalken, J. A. (2011). The mechanoreceptor TRPV4 is localized in adherence junctions of the human bladder urothelium: a morphological study. The Journal of Urology, 186(3), 1121-7. https://doi.org/10.1016/j.juro.2011.04.107
Janssen DA, et al. The Mechanoreceptor TRPV4 Is Localized in Adherence Junctions of the Human Bladder Urothelium: a Morphological Study. J Urol. 2011;186(3):1121-7. PubMed PMID: 21784462.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The mechanoreceptor TRPV4 is localized in adherence junctions of the human bladder urothelium: a morphological study. AU - Janssen,Dick A W, AU - Hoenderop,Joost G, AU - Jansen,Kees C F J, AU - Kemp,Annemiete W, AU - Heesakkers,John P F A, AU - Schalken,Jack A, Y1 - 2011/07/23/ PY - 2010/11/29/received PY - 2011/7/26/entrez PY - 2011/7/26/pubmed PY - 2011/10/28/medline SP - 1121 EP - 7 JF - The Journal of urology JO - J Urol VL - 186 IS - 3 N2 - PURPOSE: TRPV4 (transient receptor potential vanilloid 4 channel) is a nonselective cation channel involved in different sensory functions that was recently implicated in bladder mechanosensation. We investigated the cellular site of TRPV4 in bladder urothelium and explored a molecular connection between TRPV4 and urothelial adherence junctions. MATERIALS AND METHODS: We obtained healthy tissues sections from cystectomy in humans due to cancer in 3 and noncancerous conditions in 2. Besides human biopsies tissues from 7 normal and 7 TRPV4-/-mice, and the urothelial cell line RT4 were also used. Experiments were done with polyclonal antibody against TRPV4 (against the N-terminus of rat TRPV4). A molecular connection between TRPV4 and different adherence junction components was investigated using immunofluorescence, Western blot and immunoprecipitation. RESULTS: Results revealed TRPV4 on urothelial cell membranes near adherence junctions. Results were comparable in the urothelial cell line, human bladders and mouse bladders. Subsequent immunoprecipitation experiments established a molecular connection of TRPV4 to α-catenin, an integral part of the adherence junction that catenates E-cadherin to the actin-microfilament network. CONCLUSIONS: Results provide evidence for the location of TRPV4 in human bladder urothelium. TRPV4 is molecularly connected to adherence junctions on the urothelial cell membrane. TRPV4 coupling to a rigid intracellular and intercellular structural network would agree with the hypothesis that TRPV4 can be activated by bladder stretch. SN - 1527-3792 UR - https://www.unboundmedicine.com/medline/citation/21784462/The_mechanoreceptor_TRPV4_is_localized_in_adherence_junctions_of_the_human_bladder_urothelium:_a_morphological_study_ L2 - https://www.jurology.com/doi/10.1016/j.juro.2011.04.107?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -