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Mitochondria protection of baicalein against oxidative damage via induction of manganese superoxide dismutase.
Environ Toxicol Pharmacol. 2011 Jan; 31(1):233-41.ET

Abstract

This study investigated the cytoprotective effect of baicalein (5,6,7-trihydroxyflavone) against oxidative stress-induced mitochondrial dysfunction. Electron spin resonance (ESR) spectrometry revealed that baicalein showed significant scavenging effects on superoxide radicals and hydroxyl radicals. When H(2)O(2) treatment induces an increase in mitochondrial reactive oxygen species (ROS), baicalein treatment decreased high level of ROS. Baicalein significantly reduced alteration of Bcl-2 family proteins, the release of cytochrome c from mitochondria into the cytosol via inhibition of mitogen-activated protein kinase kinase-4 (MKK4/SEK1) and c-Jun NH(2)-terminal kinase (JNK) cascades induced by H(2)O(2) treatment. Manganese superoxide dismutase (MnSOD) is an important antioxidant enzyme in mitochondria against oxidative stress. Baicalein restored both MnSOD protein expression and activity, which were abolished by H(2)O(2) treatment. The transcription factor NF-E2-related factor 2 (Nrf2) is a critical regulator of MnSOD, achieved by binding to the antioxidant response element (ARE). Baicalein restored nuclear Nrf2 protein expression and its ARE binding activity, which were abolished by H(2)O(2) treatment. These studies demonstrate that baicalein attenuates mitochondrial oxidative stress by activating Nrf2-mediated MnSOD induction.

Authors+Show Affiliations

Department of Microbiology and Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21787690

Citation

Lee, In Kyung, et al. "Mitochondria Protection of Baicalein Against Oxidative Damage Via Induction of Manganese Superoxide Dismutase." Environmental Toxicology and Pharmacology, vol. 31, no. 1, 2011, pp. 233-41.
Lee IK, Kang KA, Zhang R, et al. Mitochondria protection of baicalein against oxidative damage via induction of manganese superoxide dismutase. Environ Toxicol Pharmacol. 2011;31(1):233-41.
Lee, I. K., Kang, K. A., Zhang, R., Kim, B. J., Kang, S. S., & Hyun, J. W. (2011). Mitochondria protection of baicalein against oxidative damage via induction of manganese superoxide dismutase. Environmental Toxicology and Pharmacology, 31(1), 233-41. https://doi.org/10.1016/j.etap.2010.11.002
Lee IK, et al. Mitochondria Protection of Baicalein Against Oxidative Damage Via Induction of Manganese Superoxide Dismutase. Environ Toxicol Pharmacol. 2011;31(1):233-41. PubMed PMID: 21787690.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mitochondria protection of baicalein against oxidative damage via induction of manganese superoxide dismutase. AU - Lee,In Kyung, AU - Kang,Kyoung Ah, AU - Zhang,Rui, AU - Kim,Bum Joon, AU - Kang,Sam Sik, AU - Hyun,Jin Won, Y1 - 2010/11/09/ PY - 2010/07/13/received PY - 2010/10/13/revised PY - 2010/11/01/accepted PY - 2011/7/27/entrez PY - 2011/7/27/pubmed PY - 2012/1/24/medline SP - 233 EP - 41 JF - Environmental toxicology and pharmacology JO - Environ Toxicol Pharmacol VL - 31 IS - 1 N2 - This study investigated the cytoprotective effect of baicalein (5,6,7-trihydroxyflavone) against oxidative stress-induced mitochondrial dysfunction. Electron spin resonance (ESR) spectrometry revealed that baicalein showed significant scavenging effects on superoxide radicals and hydroxyl radicals. When H(2)O(2) treatment induces an increase in mitochondrial reactive oxygen species (ROS), baicalein treatment decreased high level of ROS. Baicalein significantly reduced alteration of Bcl-2 family proteins, the release of cytochrome c from mitochondria into the cytosol via inhibition of mitogen-activated protein kinase kinase-4 (MKK4/SEK1) and c-Jun NH(2)-terminal kinase (JNK) cascades induced by H(2)O(2) treatment. Manganese superoxide dismutase (MnSOD) is an important antioxidant enzyme in mitochondria against oxidative stress. Baicalein restored both MnSOD protein expression and activity, which were abolished by H(2)O(2) treatment. The transcription factor NF-E2-related factor 2 (Nrf2) is a critical regulator of MnSOD, achieved by binding to the antioxidant response element (ARE). Baicalein restored nuclear Nrf2 protein expression and its ARE binding activity, which were abolished by H(2)O(2) treatment. These studies demonstrate that baicalein attenuates mitochondrial oxidative stress by activating Nrf2-mediated MnSOD induction. SN - 1872-7077 UR - https://www.unboundmedicine.com/medline/citation/21787690/Mitochondria_protection_of_baicalein_against_oxidative_damage_via_induction_of_manganese_superoxide_dismutase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1382-6689(10)00184-5 DB - PRIME DP - Unbound Medicine ER -