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Mutagenic and genotoxic assessment of atrazine-based herbicide to freshwater fish Channa punctatus (Bloch) using micronucleus test and single cell gel electrophoresis.
Environ Toxicol Pharmacol. 2011 Mar; 31(2):314-22.ET

Abstract

The mutagenic and genotoxic effect of 'Rasayanzine', an atrazine herbicide, was carried out in fish Channa punctatus using micronucleus and single cell gel electrophoresis (SCGE) assays. Three sub-lethal concentrations of the test material viz., SL-I (1/5th LC(50)=∼8.48 mgL(-1)), SL-II (1/8th LC(50)=∼5.30 mgL(-1)) and SL-III (1/10th LC(50)=∼4.24 mgL(-1)) were calculated using LC(50) value and the fish specimens were exposed to these concentrations. Erythrocyte and gill cells were sampled on day 0, 1, 3, 5, 7, 14, 21, 28 and 35 of exposure for assessment of micronuclei induction in blood erythrocytes and DNA damage using SCGE assay in both blood erythrocytes and gill cells. Significant effects (p<0.01) for both concentration and time of exposure were observed in treated fish. Micronuclei induction in erythrocytes was highest (8.4 mgL(-1)) on day 7 of exposure. The highest level of DNA damage in the SCGE was observed in both tissues on day 5 at all concentrations followed by gradual non-linear decline. This study further confirmed that the micronucleus and SCGE assays are useful in determining potential genotoxicity of water pollutants and might be appropriate as part of monitoring program.

Authors+Show Affiliations

Department of Applied Biology, Ebonyi State University, PMB 053, Abakaliki, Ebonyi State, Nigeria.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21787700

Citation

Nwani, C D., et al. "Mutagenic and Genotoxic Assessment of Atrazine-based Herbicide to Freshwater Fish Channa Punctatus (Bloch) Using Micronucleus Test and Single Cell Gel Electrophoresis." Environmental Toxicology and Pharmacology, vol. 31, no. 2, 2011, pp. 314-22.
Nwani CD, Nagpure NS, Kumar R, et al. Mutagenic and genotoxic assessment of atrazine-based herbicide to freshwater fish Channa punctatus (Bloch) using micronucleus test and single cell gel electrophoresis. Environ Toxicol Pharmacol. 2011;31(2):314-22.
Nwani, C. D., Nagpure, N. S., Kumar, R., Kushwaha, B., Kumar, P., & Lakra, W. S. (2011). Mutagenic and genotoxic assessment of atrazine-based herbicide to freshwater fish Channa punctatus (Bloch) using micronucleus test and single cell gel electrophoresis. Environmental Toxicology and Pharmacology, 31(2), 314-22. https://doi.org/10.1016/j.etap.2010.12.001
Nwani CD, et al. Mutagenic and Genotoxic Assessment of Atrazine-based Herbicide to Freshwater Fish Channa Punctatus (Bloch) Using Micronucleus Test and Single Cell Gel Electrophoresis. Environ Toxicol Pharmacol. 2011;31(2):314-22. PubMed PMID: 21787700.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutagenic and genotoxic assessment of atrazine-based herbicide to freshwater fish Channa punctatus (Bloch) using micronucleus test and single cell gel electrophoresis. AU - Nwani,C D, AU - Nagpure,N S, AU - Kumar,Ravindra, AU - Kushwaha,Basdeo, AU - Kumar,Pavan, AU - Lakra,W S, Y1 - 2010/12/17/ PY - 2010/05/20/received PY - 2010/12/08/revised PY - 2010/12/10/accepted PY - 2011/7/27/entrez PY - 2011/7/27/pubmed PY - 2012/2/24/medline SP - 314 EP - 22 JF - Environmental toxicology and pharmacology JO - Environ Toxicol Pharmacol VL - 31 IS - 2 N2 - The mutagenic and genotoxic effect of 'Rasayanzine', an atrazine herbicide, was carried out in fish Channa punctatus using micronucleus and single cell gel electrophoresis (SCGE) assays. Three sub-lethal concentrations of the test material viz., SL-I (1/5th LC(50)=∼8.48 mgL(-1)), SL-II (1/8th LC(50)=∼5.30 mgL(-1)) and SL-III (1/10th LC(50)=∼4.24 mgL(-1)) were calculated using LC(50) value and the fish specimens were exposed to these concentrations. Erythrocyte and gill cells were sampled on day 0, 1, 3, 5, 7, 14, 21, 28 and 35 of exposure for assessment of micronuclei induction in blood erythrocytes and DNA damage using SCGE assay in both blood erythrocytes and gill cells. Significant effects (p<0.01) for both concentration and time of exposure were observed in treated fish. Micronuclei induction in erythrocytes was highest (8.4 mgL(-1)) on day 7 of exposure. The highest level of DNA damage in the SCGE was observed in both tissues on day 5 at all concentrations followed by gradual non-linear decline. This study further confirmed that the micronucleus and SCGE assays are useful in determining potential genotoxicity of water pollutants and might be appropriate as part of monitoring program. SN - 1872-7077 UR - https://www.unboundmedicine.com/medline/citation/21787700/Mutagenic_and_genotoxic_assessment_of_atrazine_based_herbicide_to_freshwater_fish_Channa_punctatus__Bloch__using_micronucleus_test_and_single_cell_gel_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1382-6689(10)00208-5 DB - PRIME DP - Unbound Medicine ER -