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Antiherpetic activity of a sulfated polysaccharide from Agaricus brasiliensis mycelia.
Antiviral Res. 2011 Oct; 92(1):108-14.AR

Abstract

Sulfated polysaccharides are good candidates for drug discovery in the treatment of herpetic infections. Agaricus brasiliensis (syn A. subrufescens, A. blazei) is a Basidiomycete fungus native to the Atlantic forest region of Southeastern Brazil. Herein we report the chemical modification of a polysaccharide extracted from A. brasiliensis mycelia to obtain its sulfated derivative (MI-S), which presented a promising inhibitory activity against HSV-1 [KOS and 29R (acyclovir-resistant) strains] and HSV-2 strain 333, with selectivity indices (SI = CC50/IC50) higher than 439, 208, and 562, respectively. The mechanisms underlying this inhibitory activity were scrutinized by plaque assay with different methodological strategies. MI-S had no virucidal effects, but inhibited HSV-1 and HSV-2 attachment, penetration, and cell-to-cell spread, as well as reducing the expression of HSV-1 ICP27, UL42, gB, and gD proteins. MI-S also presented synergistic antiviral effect with acyclovir. These results suggest that MI-S presents multiple modes of anti-HSV action.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Cardozo FT
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
Camelini CM
No affiliation info available
Mascarello A
No affiliation info available
Rossi MJ
No affiliation info available
Nunes RJ
No affiliation info available
Barardi CR
No affiliation info available
de Mendonça MM
No affiliation info available
Simões CM
No affiliation info available

MeSH

AgaricusAnimalsAntiviral AgentsChlorocebus aethiopsHerpes SimplexHerpesvirus 1, HumanHerpesvirus 2, HumanHumansMyceliumPolysaccharidesVero Cells

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21787804

Citation

Cardozo, Francielle Tramontini Gomes de Sousa, et al. "Antiherpetic Activity of a Sulfated Polysaccharide From Agaricus Brasiliensis Mycelia." Antiviral Research, vol. 92, no. 1, 2011, pp. 108-14.
Cardozo FT, Camelini CM, Mascarello A, et al. Antiherpetic activity of a sulfated polysaccharide from Agaricus brasiliensis mycelia. Antiviral Res. 2011;92(1):108-14.
Cardozo, F. T., Camelini, C. M., Mascarello, A., Rossi, M. J., Nunes, R. J., Barardi, C. R., de Mendonça, M. M., & Simões, C. M. (2011). Antiherpetic activity of a sulfated polysaccharide from Agaricus brasiliensis mycelia. Antiviral Research, 92(1), 108-14. https://doi.org/10.1016/j.antiviral.2011.07.009
Cardozo FT, et al. Antiherpetic Activity of a Sulfated Polysaccharide From Agaricus Brasiliensis Mycelia. Antiviral Res. 2011;92(1):108-14. PubMed PMID: 21787804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiherpetic activity of a sulfated polysaccharide from Agaricus brasiliensis mycelia. AU - Cardozo,Francielle Tramontini Gomes de Sousa, AU - Camelini,Carla Maísa, AU - Mascarello,Alessandra, AU - Rossi,Márcio José, AU - Nunes,Ricardo José, AU - Barardi,Célia Regina Monte, AU - de Mendonça,Margarida Matos, AU - Simões,Cláudia Maria Oliveira, Y1 - 2011/07/20/ PY - 2011/02/27/received PY - 2011/05/18/revised PY - 2011/07/08/accepted PY - 2011/7/27/entrez PY - 2011/7/27/pubmed PY - 2012/1/6/medline SP - 108 EP - 14 JF - Antiviral research JO - Antiviral Res VL - 92 IS - 1 N2 - Sulfated polysaccharides are good candidates for drug discovery in the treatment of herpetic infections. Agaricus brasiliensis (syn A. subrufescens, A. blazei) is a Basidiomycete fungus native to the Atlantic forest region of Southeastern Brazil. Herein we report the chemical modification of a polysaccharide extracted from A. brasiliensis mycelia to obtain its sulfated derivative (MI-S), which presented a promising inhibitory activity against HSV-1 [KOS and 29R (acyclovir-resistant) strains] and HSV-2 strain 333, with selectivity indices (SI = CC50/IC50) higher than 439, 208, and 562, respectively. The mechanisms underlying this inhibitory activity were scrutinized by plaque assay with different methodological strategies. MI-S had no virucidal effects, but inhibited HSV-1 and HSV-2 attachment, penetration, and cell-to-cell spread, as well as reducing the expression of HSV-1 ICP27, UL42, gB, and gD proteins. MI-S also presented synergistic antiviral effect with acyclovir. These results suggest that MI-S presents multiple modes of anti-HSV action. SN - 1872-9096 UR - https://www.unboundmedicine.com/medline/citation/21787804/Antiherpetic_activity_of_a_sulfated_polysaccharide_from_Agaricus_brasiliensis_mycelia_ DB - PRIME DP - Unbound Medicine ER -