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Nonstructural protein 1 antibody-based epitope-blocking enzyme-linked immunosorbent assay to differentiate Japanese encephalitis virus from dengue virus infections in humans.
Jpn J Infect Dis. 2011; 64(4):284-91.JJ

Abstract

Japanese encephalitis virus (JEV) and the four dengue viruses (DENV1-4) are co-distributed in Southeast and South Asia. Since JEV is antigenically cross-reactive with DENV1-4, the differentiation between these viruses using antibody assays may be difficult. Herein, we describe the development of an epitope-blocking enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody specific for the nonstructural protein 1 (NS1) of JEV (JEV-NS1) to differentiate antibodies against JEV from those against DENV1-4. Hyperimmune mouse sera against JEV-NS1 showed >60% inhibition, whereas those against NS1 of DENV1-4 showed <30% inhibition. The present assay could therefore detect antibodies specific for JEV. For testing of human sera, a temporary cutoff value (30.8%) was calculated the average and standard deviation obtained for sera of control humans negative for JEV antibodies. Human sera positive for antibodies to any of DENV1-4 NS1 but negative for antibodies to JEV-NS1 showed a lower percentage inhibition than the cutoff value. On the other hand, sera with JEV-NS1 antibody levels of ≥0.400, as determined by the conventional ELISA (medially/strongly positive for JEV-NS1 antibodies), showed percentage inhibition greater than the cutoff. Although this blocking ELISA afforded false-negative results for most sera that were weakly positive for JEV-NS1 antibodies, it may be useful for investigating the seroepidemiology of JEV antibodies in dengue-endemic areas.

Authors+Show Affiliations

Division of Infectious Diseases, Department of International Health, Kobe University Graduate School of Health Sciences, Kobe 654-0142, Japan. ekon@kobe-u.ac.jpNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21788702

Citation

Konishi, Eiji, and Mayu Konishi. "Nonstructural Protein 1 Antibody-based Epitope-blocking Enzyme-linked Immunosorbent Assay to Differentiate Japanese Encephalitis Virus From Dengue Virus Infections in Humans." Japanese Journal of Infectious Diseases, vol. 64, no. 4, 2011, pp. 284-91.
Konishi E, Konishi M. Nonstructural protein 1 antibody-based epitope-blocking enzyme-linked immunosorbent assay to differentiate Japanese encephalitis virus from dengue virus infections in humans. Jpn J Infect Dis. 2011;64(4):284-91.
Konishi, E., & Konishi, M. (2011). Nonstructural protein 1 antibody-based epitope-blocking enzyme-linked immunosorbent assay to differentiate Japanese encephalitis virus from dengue virus infections in humans. Japanese Journal of Infectious Diseases, 64(4), 284-91.
Konishi E, Konishi M. Nonstructural Protein 1 Antibody-based Epitope-blocking Enzyme-linked Immunosorbent Assay to Differentiate Japanese Encephalitis Virus From Dengue Virus Infections in Humans. Jpn J Infect Dis. 2011;64(4):284-91. PubMed PMID: 21788702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nonstructural protein 1 antibody-based epitope-blocking enzyme-linked immunosorbent assay to differentiate Japanese encephalitis virus from dengue virus infections in humans. AU - Konishi,Eiji, AU - Konishi,Mayu, PY - 2011/7/27/entrez PY - 2011/7/27/pubmed PY - 2011/12/13/medline SP - 284 EP - 91 JF - Japanese journal of infectious diseases JO - Jpn J Infect Dis VL - 64 IS - 4 N2 - Japanese encephalitis virus (JEV) and the four dengue viruses (DENV1-4) are co-distributed in Southeast and South Asia. Since JEV is antigenically cross-reactive with DENV1-4, the differentiation between these viruses using antibody assays may be difficult. Herein, we describe the development of an epitope-blocking enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody specific for the nonstructural protein 1 (NS1) of JEV (JEV-NS1) to differentiate antibodies against JEV from those against DENV1-4. Hyperimmune mouse sera against JEV-NS1 showed >60% inhibition, whereas those against NS1 of DENV1-4 showed <30% inhibition. The present assay could therefore detect antibodies specific for JEV. For testing of human sera, a temporary cutoff value (30.8%) was calculated the average and standard deviation obtained for sera of control humans negative for JEV antibodies. Human sera positive for antibodies to any of DENV1-4 NS1 but negative for antibodies to JEV-NS1 showed a lower percentage inhibition than the cutoff value. On the other hand, sera with JEV-NS1 antibody levels of ≥0.400, as determined by the conventional ELISA (medially/strongly positive for JEV-NS1 antibodies), showed percentage inhibition greater than the cutoff. Although this blocking ELISA afforded false-negative results for most sera that were weakly positive for JEV-NS1 antibodies, it may be useful for investigating the seroepidemiology of JEV antibodies in dengue-endemic areas. SN - 1884-2836 UR - https://www.unboundmedicine.com/medline/citation/21788702/Nonstructural_protein_1_antibody_based_epitope_blocking_enzyme_linked_immunosorbent_assay_to_differentiate_Japanese_encephalitis_virus_from_dengue_virus_infections_in_humans_ L2 - http://www.diseaseinfosearch.org/result/3899 DB - PRIME DP - Unbound Medicine ER -