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Characterization of cellobiohydrolase from a newly isolated strain of Agaricus arvencis.
J Microbiol Biotechnol. 2011 Jul; 21(7):711-8.JM

Abstract

A highly efficient cellobiohydrolase (CBH)-secreting basidiomycetous fungus, Agaricus arvensis KMJ623, was isolated and identified based on its morphological features and sequence analysis of internal transcribed spacer rDNA. An extracellular CBH was purified to homogeneity from A. arvencis culture supernatant using sequential chromatography. The relative molecular mass of A. arvencis CBH was determined to be 65 kDa by SDSPAGE and 130 kDa by size-exclusion chromatography, indicating that the enzyme is a dimer. A. arvencis CBH showed a catalytic efficiency (kcat/Km) of 31.8 mM⁻¹ s⁻¹ for p-nitrophenyl-beta-D-cellobioside, the highest level seen for CBH-producing microorganisms. Its internal amino acid sequences showed significant homology with CBHs from glycoside hydrolase family 7. Although CBHs have been purified and characterized from other sources, A. arvencis CBH is distinguished from other CBHs by its high catalytic efficiency.

Authors+Show Affiliations

Department of Chemical Engineering, Konkuk University, Seoul 143-701, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21791957

Citation

Lee, Kyung-Min, et al. "Characterization of Cellobiohydrolase From a Newly Isolated Strain of Agaricus Arvencis." Journal of Microbiology and Biotechnology, vol. 21, no. 7, 2011, pp. 711-8.
Lee KM, Moon HJ, Kalyani D, et al. Characterization of cellobiohydrolase from a newly isolated strain of Agaricus arvencis. J Microbiol Biotechnol. 2011;21(7):711-8.
Lee, K. M., Moon, H. J., Kalyani, D., Kim, H., Kim, I. W., Jeya, M., & Lee, J. K. (2011). Characterization of cellobiohydrolase from a newly isolated strain of Agaricus arvencis. Journal of Microbiology and Biotechnology, 21(7), 711-8.
Lee KM, et al. Characterization of Cellobiohydrolase From a Newly Isolated Strain of Agaricus Arvencis. J Microbiol Biotechnol. 2011;21(7):711-8. PubMed PMID: 21791957.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of cellobiohydrolase from a newly isolated strain of Agaricus arvencis. AU - Lee,Kyung-Min, AU - Moon,Hee-Jung, AU - Kalyani,Dayanand, AU - Kim,Hoon, AU - Kim,In-Won, AU - Jeya,Marimuthu, AU - Lee,Jung-Kul, PY - 2011/7/28/entrez PY - 2011/7/28/pubmed PY - 2011/11/16/medline SP - 711 EP - 8 JF - Journal of microbiology and biotechnology JO - J. Microbiol. Biotechnol. VL - 21 IS - 7 N2 - A highly efficient cellobiohydrolase (CBH)-secreting basidiomycetous fungus, Agaricus arvensis KMJ623, was isolated and identified based on its morphological features and sequence analysis of internal transcribed spacer rDNA. An extracellular CBH was purified to homogeneity from A. arvencis culture supernatant using sequential chromatography. The relative molecular mass of A. arvencis CBH was determined to be 65 kDa by SDSPAGE and 130 kDa by size-exclusion chromatography, indicating that the enzyme is a dimer. A. arvencis CBH showed a catalytic efficiency (kcat/Km) of 31.8 mM⁻¹ s⁻¹ for p-nitrophenyl-beta-D-cellobioside, the highest level seen for CBH-producing microorganisms. Its internal amino acid sequences showed significant homology with CBHs from glycoside hydrolase family 7. Although CBHs have been purified and characterized from other sources, A. arvencis CBH is distinguished from other CBHs by its high catalytic efficiency. SN - 1738-8872 UR - https://www.unboundmedicine.com/medline/citation/21791957/Characterization_of_cellobiohydrolase_from_a_newly_isolated_strain_of_Agaricus_arvencis_ L2 - http://www.jmb.or.kr/journal/viewJournal.html?year=2011&vol=21&num=7&page=711 DB - PRIME DP - Unbound Medicine ER -