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Microsatellite mapping of Ae. speltoides and map-based comparative analysis of the S, G, and B genomes of Triticeae species.
Theor Appl Genet. 2011 Nov; 123(7):1145-57.TA

Abstract

The first microsatellite linkage map of Ae. speltoides Tausch (2n = 2x = 14, SS), which is a wild species with a genome closely related to the B and G genomes of polyploid wheats, was developed based on two F(2) mapping populations using microsatellite (SSR) markers from Ae. speltoides, wheat genomic SSRs (g-SSRs) and EST-derived SSRs. A total of 144 different microsatellite loci were mapped in the Ae. speltoides genome. The transferability of the SSRs markers between the related S, B, and G genomes allowed possible integration of new markers into the T. timopheevii G genome chromosomal maps and map-based comparisons. Thirty-one new microsatellite loci assigned to the genetic framework of the T. timopheevii G genome maps were composed of wheat g-SSR (genomic SSR) markers. Most of the used Ae. speltoides SSRs were mapped onto chromosomes of the G genome supporting a close relationship between the G and S genomes. Comparative microsatellite mapping of the S, B, and G genomes demonstrated colinearity between the chromosomes within homoeologous groups, except for intergenomic T6A(t)S.1G, T4AL.5AL.7BS translocations. A translocation between chromosomes 2 and 6 that is present in the T. aestivum B genome was found in neither Ae. speltoides nor in T. timopheevii. Although the marker order was generally conserved among the B, S, and G genomes, the total length of the Ae. speltoides chromosomal maps and the genetic distances between homoeologous loci located in the proximal regions of the S genome chromosomes were reduced compared with the B, and G genome chromosomes.

Authors+Show Affiliations

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Lavrentieva Ave. 10, Novosibirsk, 630090, Russia. oxanad@bionet.nsc.ruNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21792632

Citation

Dobrovolskaya, O, et al. "Microsatellite Mapping of Ae. Speltoides and Map-based Comparative Analysis of the S, G, and B Genomes of Triticeae Species." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 123, no. 7, 2011, pp. 1145-57.
Dobrovolskaya O, Boeuf C, Salse J, et al. Microsatellite mapping of Ae. speltoides and map-based comparative analysis of the S, G, and B genomes of Triticeae species. Theor Appl Genet. 2011;123(7):1145-57.
Dobrovolskaya, O., Boeuf, C., Salse, J., Pont, C., Sourdille, P., Bernard, M., & Salina, E. (2011). Microsatellite mapping of Ae. speltoides and map-based comparative analysis of the S, G, and B genomes of Triticeae species. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 123(7), 1145-57. https://doi.org/10.1007/s00122-011-1655-z
Dobrovolskaya O, et al. Microsatellite Mapping of Ae. Speltoides and Map-based Comparative Analysis of the S, G, and B Genomes of Triticeae Species. Theor Appl Genet. 2011;123(7):1145-57. PubMed PMID: 21792632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microsatellite mapping of Ae. speltoides and map-based comparative analysis of the S, G, and B genomes of Triticeae species. AU - Dobrovolskaya,O, AU - Boeuf,C, AU - Salse,J, AU - Pont,C, AU - Sourdille,P, AU - Bernard,M, AU - Salina,E, Y1 - 2011/07/27/ PY - 2008/08/24/received PY - 2011/07/09/accepted PY - 2011/7/28/entrez PY - 2011/7/28/pubmed PY - 2012/3/6/medline SP - 1145 EP - 57 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 123 IS - 7 N2 - The first microsatellite linkage map of Ae. speltoides Tausch (2n = 2x = 14, SS), which is a wild species with a genome closely related to the B and G genomes of polyploid wheats, was developed based on two F(2) mapping populations using microsatellite (SSR) markers from Ae. speltoides, wheat genomic SSRs (g-SSRs) and EST-derived SSRs. A total of 144 different microsatellite loci were mapped in the Ae. speltoides genome. The transferability of the SSRs markers between the related S, B, and G genomes allowed possible integration of new markers into the T. timopheevii G genome chromosomal maps and map-based comparisons. Thirty-one new microsatellite loci assigned to the genetic framework of the T. timopheevii G genome maps were composed of wheat g-SSR (genomic SSR) markers. Most of the used Ae. speltoides SSRs were mapped onto chromosomes of the G genome supporting a close relationship between the G and S genomes. Comparative microsatellite mapping of the S, B, and G genomes demonstrated colinearity between the chromosomes within homoeologous groups, except for intergenomic T6A(t)S.1G, T4AL.5AL.7BS translocations. A translocation between chromosomes 2 and 6 that is present in the T. aestivum B genome was found in neither Ae. speltoides nor in T. timopheevii. Although the marker order was generally conserved among the B, S, and G genomes, the total length of the Ae. speltoides chromosomal maps and the genetic distances between homoeologous loci located in the proximal regions of the S genome chromosomes were reduced compared with the B, and G genome chromosomes. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/21792632/Microsatellite_mapping_of_Ae__speltoides_and_map_based_comparative_analysis_of_the_S_G_and_B_genomes_of_Triticeae_species_ L2 - https://dx.doi.org/10.1007/s00122-011-1655-z DB - PRIME DP - Unbound Medicine ER -