Tags

Type your tag names separated by a space and hit enter

Robust and sensitive high-performance liquid chromatographic-UV detection technique for the determination of tigecycline in rabbit plasma.
J AOAC Int. 2011 May-Jun; 94(3):847-56.JA

Abstract

An isocratic HPLC method with detection at 248 nm was developed and fully validated for the determination of tigecycline in rabbit plasma. Minocycline was used as an internal standard. A Hypersil BDS RP-C18 column (250 x 4.6 mm, 5 microm particle size) was used with the mobile phase phosphate buffer (pH 7.10, 0.070 M)-acetonitrile (76 + 24, v/v) at a flow rate of 1.0 mL/min. The elution time of tigecycline and minocycline was approximately 8.1 and 9.9 min, respectively. Calibration curves of tigecycline were linear in the concentration range of 0.021-3.15 microg/mL in plasma. The LOD and LOQ in plasma were estimated as 7 and 21 ng/mL, respectively. The intraday and interday precision values of the method were in the range of 5.0-7.1 and 5.6-9.1%, while the corresponding accuracy values were in the ranges of 92.8-111.1 and 97.6-102.3%, respectively. At the LOQ, the intraday precision was 18.7%, while intraday and interday accuracy values were 97.3 and 98.0%, respectively. Robustness of the proposed method was studied using a Plackett-Burman experimental design. A pharmacokinetic profile is presented for confirmation of the applicability of the method to pharmacokinetic studies.

Authors+Show Affiliations

University of Athens, Department of Chemistry, Laboratory of Analytical Chemistry, 15771 Athens, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21797013

Citation

Zorpas, Kostas M., et al. "Robust and Sensitive High-performance Liquid chromatographic-UV Detection Technique for the Determination of Tigecycline in Rabbit Plasma." Journal of AOAC International, vol. 94, no. 3, 2011, pp. 847-56.
Zorpas KM, Valsami GN, Vryonis EV, et al. Robust and sensitive high-performance liquid chromatographic-UV detection technique for the determination of tigecycline in rabbit plasma. J AOAC Int. 2011;94(3):847-56.
Zorpas, K. M., Valsami, G. N., Vryonis, E. V., Skoutelis, A. T., & Archontaki, H. A. (2011). Robust and sensitive high-performance liquid chromatographic-UV detection technique for the determination of tigecycline in rabbit plasma. Journal of AOAC International, 94(3), 847-56.
Zorpas KM, et al. Robust and Sensitive High-performance Liquid chromatographic-UV Detection Technique for the Determination of Tigecycline in Rabbit Plasma. J AOAC Int. 2011 May-Jun;94(3):847-56. PubMed PMID: 21797013.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Robust and sensitive high-performance liquid chromatographic-UV detection technique for the determination of tigecycline in rabbit plasma. AU - Zorpas,Kostas M, AU - Valsami,Georgia N, AU - Vryonis,Evangelos V, AU - Skoutelis,Athanasios T, AU - Archontaki,Helen A, PY - 2011/7/30/entrez PY - 2011/7/30/pubmed PY - 2011/8/24/medline SP - 847 EP - 56 JF - Journal of AOAC International JO - J AOAC Int VL - 94 IS - 3 N2 - An isocratic HPLC method with detection at 248 nm was developed and fully validated for the determination of tigecycline in rabbit plasma. Minocycline was used as an internal standard. A Hypersil BDS RP-C18 column (250 x 4.6 mm, 5 microm particle size) was used with the mobile phase phosphate buffer (pH 7.10, 0.070 M)-acetonitrile (76 + 24, v/v) at a flow rate of 1.0 mL/min. The elution time of tigecycline and minocycline was approximately 8.1 and 9.9 min, respectively. Calibration curves of tigecycline were linear in the concentration range of 0.021-3.15 microg/mL in plasma. The LOD and LOQ in plasma were estimated as 7 and 21 ng/mL, respectively. The intraday and interday precision values of the method were in the range of 5.0-7.1 and 5.6-9.1%, while the corresponding accuracy values were in the ranges of 92.8-111.1 and 97.6-102.3%, respectively. At the LOQ, the intraday precision was 18.7%, while intraday and interday accuracy values were 97.3 and 98.0%, respectively. Robustness of the proposed method was studied using a Plackett-Burman experimental design. A pharmacokinetic profile is presented for confirmation of the applicability of the method to pharmacokinetic studies. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/21797013/Robust_and_sensitive_high_performance_liquid_chromatographic_UV_detection_technique_for_the_determination_of_tigecycline_in_rabbit_plasma_ L2 - https://medlineplus.gov/antibiotics.html DB - PRIME DP - Unbound Medicine ER -