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Gold nanoparticle-based homogeneous fluorescent aptasensor for multiplex detection.
Analyst. 2011 Sep 21; 136(18):3720-4.A

Abstract

We developed a homogeneous fluorescence assay for multiplex detection based on the target induced conformational change of DNA aptamers. DNA aptamers were immobilized on quantum dots (QDs), and QDs conjugated ssDNA was adsorbed on the surface of gold nanoparticles (AuNPs) by electrostatic interaction between uncoiled ssDNA and the AuNPs. Subsequently the fluorescence of QDs was effectively quenched by the AuNPs due to fluorescence resonance energy transfer (FRET) of QDs to AuNPs. In the presence of targets, the QDs conjugated aptamers were detached from AuNPs by target induced conformational change of aptamers, consequently the fluorescence of the QDs was recovered proportional to the target concentration. In this study, three different QD/aptamer conjugates were used for multiplex detection of mercury ions, adenosine and potassium ions. In a control experiment, all of the three targets were simultaneously detected with high selectivity.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Kim YS
Environment Division, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul, 136-791, South Korea.
Jurng J
No affiliation info available

MeSH

AdenosineAptamers, NucleotideDNA, Single-StrandedFluorescence Resonance Energy TransferGoldIonsMercuryMetal NanoparticlesPotassiumQuantum DotsStatic Electricity

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21799952

Citation

Kim, Yeon Seok, and Jongsoo Jurng. "Gold Nanoparticle-based Homogeneous Fluorescent Aptasensor for Multiplex Detection." The Analyst, vol. 136, no. 18, 2011, pp. 3720-4.
Kim YS, Jurng J. Gold nanoparticle-based homogeneous fluorescent aptasensor for multiplex detection. Analyst. 2011;136(18):3720-4.
Kim, Y. S., & Jurng, J. (2011). Gold nanoparticle-based homogeneous fluorescent aptasensor for multiplex detection. The Analyst, 136(18), 3720-4. https://doi.org/10.1039/c1an15261k
Kim YS, Jurng J. Gold Nanoparticle-based Homogeneous Fluorescent Aptasensor for Multiplex Detection. Analyst. 2011 Sep 21;136(18):3720-4. PubMed PMID: 21799952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gold nanoparticle-based homogeneous fluorescent aptasensor for multiplex detection. AU - Kim,Yeon Seok, AU - Jurng,Jongsoo, Y1 - 2011/07/28/ PY - 2011/7/30/entrez PY - 2011/7/30/pubmed PY - 2011/12/15/medline SP - 3720 EP - 4 JF - The Analyst JO - Analyst VL - 136 IS - 18 N2 - We developed a homogeneous fluorescence assay for multiplex detection based on the target induced conformational change of DNA aptamers. DNA aptamers were immobilized on quantum dots (QDs), and QDs conjugated ssDNA was adsorbed on the surface of gold nanoparticles (AuNPs) by electrostatic interaction between uncoiled ssDNA and the AuNPs. Subsequently the fluorescence of QDs was effectively quenched by the AuNPs due to fluorescence resonance energy transfer (FRET) of QDs to AuNPs. In the presence of targets, the QDs conjugated aptamers were detached from AuNPs by target induced conformational change of aptamers, consequently the fluorescence of the QDs was recovered proportional to the target concentration. In this study, three different QD/aptamer conjugates were used for multiplex detection of mercury ions, adenosine and potassium ions. In a control experiment, all of the three targets were simultaneously detected with high selectivity. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/21799952/Gold_nanoparticle_based_homogeneous_fluorescent_aptasensor_for_multiplex_detection_ L2 - https://doi.org/10.1039/c1an15261k DB - PRIME DP - Unbound Medicine ER -