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Hepatitis E virus in domestic pigs and surface waters in Slovenia: prevalence and molecular characterization of a novel genotype 3 lineage.
Infect Genet Evol. 2011 Oct; 11(7):1732-7.IG

Abstract

A novel hepatitis E virus (HEV) genotype 3 lineage was identified in Slovenian pig herds. Stool samples from six Slovenian pig farms were collected and tested for the presence of HEV RNA. Of 85 individual samples 15 (20.3%) were positive for HEV RNA of which 2/38 (5.3%), 6/21 (28.6%) and 7/26 (26.9%) were from suckling, weanling and fattening pigs, respectively. Additionally, 51 pooled porcine stool samples were tested in one of the biggest pig farm and the estimated infection rate of individual pig was calculated, resulting in 7.8%, 10.6% and 24.2% for suckling, weanling and fattening pigs, respectively. The majority of HEV positive porcine samples were from the same pig farm. Out of 17 Slovenian patients with confirmed recent hepatitis E in the period 1999-2011, the serum samples of 10 patients were tested and 3 samples turned out to be HEV RNA positive. Furthermore, 60 surface water samples were tested throughout the country, of which 2 (3.3%) were positive for HEV RNA, one of them in the near vicinity of a pig farm. All HEV strains were analysed at 5' ORF1 and 5' ORF2 regions and both genome regions confirmed that Slovenian HEV strains represent a distinct genotype 3 lineage, diverse from all other genotype 3 lineages available in GenBank and described in the literature to date. All but one HEV strains detected in pigs in Slovenia represent a monophyletic branch in phylogenetic trees, with a high degree of sequence identity. One human HEV strain belonged to genotype 1 and two to genotype 3 but did not match the new genotype 3 lineage detected in Slovenian pig herds.

Authors+Show Affiliations

Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloška 4, SI-1000 Ljubljana, Slovenia. andrej.steyer@mf.uni-lj.siNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21802527

Citation

Steyer, Andrej, et al. "Hepatitis E Virus in Domestic Pigs and Surface Waters in Slovenia: Prevalence and Molecular Characterization of a Novel Genotype 3 Lineage." Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, vol. 11, no. 7, 2011, pp. 1732-7.
Steyer A, Naglič T, Močilnik T, et al. Hepatitis E virus in domestic pigs and surface waters in Slovenia: prevalence and molecular characterization of a novel genotype 3 lineage. Infect Genet Evol. 2011;11(7):1732-7.
Steyer, A., Naglič, T., Močilnik, T., Poljšak-Prijatelj, M., & Poljak, M. (2011). Hepatitis E virus in domestic pigs and surface waters in Slovenia: prevalence and molecular characterization of a novel genotype 3 lineage. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 11(7), 1732-7. https://doi.org/10.1016/j.meegid.2011.07.007
Steyer A, et al. Hepatitis E Virus in Domestic Pigs and Surface Waters in Slovenia: Prevalence and Molecular Characterization of a Novel Genotype 3 Lineage. Infect Genet Evol. 2011;11(7):1732-7. PubMed PMID: 21802527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatitis E virus in domestic pigs and surface waters in Slovenia: prevalence and molecular characterization of a novel genotype 3 lineage. AU - Steyer,Andrej, AU - Naglič,Tina, AU - Močilnik,Tina, AU - Poljšak-Prijatelj,Mateja, AU - Poljak,Mario, Y1 - 2011/07/23/ PY - 2011/04/18/received PY - 2011/07/05/revised PY - 2011/07/10/accepted PY - 2011/8/2/entrez PY - 2011/8/2/pubmed PY - 2012/3/1/medline SP - 1732 EP - 7 JF - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JO - Infect Genet Evol VL - 11 IS - 7 N2 - A novel hepatitis E virus (HEV) genotype 3 lineage was identified in Slovenian pig herds. Stool samples from six Slovenian pig farms were collected and tested for the presence of HEV RNA. Of 85 individual samples 15 (20.3%) were positive for HEV RNA of which 2/38 (5.3%), 6/21 (28.6%) and 7/26 (26.9%) were from suckling, weanling and fattening pigs, respectively. Additionally, 51 pooled porcine stool samples were tested in one of the biggest pig farm and the estimated infection rate of individual pig was calculated, resulting in 7.8%, 10.6% and 24.2% for suckling, weanling and fattening pigs, respectively. The majority of HEV positive porcine samples were from the same pig farm. Out of 17 Slovenian patients with confirmed recent hepatitis E in the period 1999-2011, the serum samples of 10 patients were tested and 3 samples turned out to be HEV RNA positive. Furthermore, 60 surface water samples were tested throughout the country, of which 2 (3.3%) were positive for HEV RNA, one of them in the near vicinity of a pig farm. All HEV strains were analysed at 5' ORF1 and 5' ORF2 regions and both genome regions confirmed that Slovenian HEV strains represent a distinct genotype 3 lineage, diverse from all other genotype 3 lineages available in GenBank and described in the literature to date. All but one HEV strains detected in pigs in Slovenia represent a monophyletic branch in phylogenetic trees, with a high degree of sequence identity. One human HEV strain belonged to genotype 1 and two to genotype 3 but did not match the new genotype 3 lineage detected in Slovenian pig herds. SN - 1567-7257 UR - https://www.unboundmedicine.com/medline/citation/21802527/Hepatitis_E_virus_in_domestic_pigs_and_surface_waters_in_Slovenia:_prevalence_and_molecular_characterization_of_a_novel_genotype_3_lineage_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-1348(11)00261-9 DB - PRIME DP - Unbound Medicine ER -