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Extreme calorie restriction and energy source starvation in Saccharomyces cerevisiae represent distinct physiological states.
Biochim Biophys Acta. 2011 Dec; 1813(12):2133-44.BB

Abstract

Cultivation methods used to investigate microbial calorie restriction often result in carbon and energy starvation. This study aims to dissect cellular responses to calorie restriction and starvation in Saccharomyces cerevisiae by using retentostat cultivation. In retentostats, cells are continuously supplied with a small, constant carbon and energy supply, sufficient for maintenance of cellular viability and integrity but insufficient for growth. When glucose-limited retentostats cultivated under extreme calorie restriction were subjected to glucose starvation, calorie-restricted and glucose-starved cells were found to share characteristics such as increased heat-shock tolerance and expression of quiescence-related genes. However, they also displayed strikingly different features. While calorie-restricted yeast cultures remained metabolically active and viable for prolonged periods of time, glucose starvation resulted in rapid consumption of reserve carbohydrates, population heterogeneity due to appearance of senescent cells and, ultimately, loss of viability. Moreover, during starvation, calculated rates of ATP synthesis from reserve carbohydrates were 2-3 orders of magnitude lower than steady-state ATP-turnover rates calculated under extreme calorie restriction in retentostats. Stringent reduction of ATP turnover during glucose starvation was accompanied by a strong down-regulation of genes involved in protein synthesis. These results demonstrate that extreme calorie restriction and carbon starvation represent different physiological states in S. cerevisiae.

Authors+Show Affiliations

Kluyver Centre for Genomics of Industrial Fermentation, Julianalaan 67, 2628 BC Delft, The Netherlands; Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21803078

Citation

Boender, Léonie G M., et al. "Extreme Calorie Restriction and Energy Source Starvation in Saccharomyces Cerevisiae Represent Distinct Physiological States." Biochimica Et Biophysica Acta, vol. 1813, no. 12, 2011, pp. 2133-44.
Boender LG, Almering MJ, Dijk M, et al. Extreme calorie restriction and energy source starvation in Saccharomyces cerevisiae represent distinct physiological states. Biochim Biophys Acta. 2011;1813(12):2133-44.
Boender, L. G., Almering, M. J., Dijk, M., van Maris, A. J., de Winde, J. H., Pronk, J. T., & Daran-Lapujade, P. (2011). Extreme calorie restriction and energy source starvation in Saccharomyces cerevisiae represent distinct physiological states. Biochimica Et Biophysica Acta, 1813(12), 2133-44. https://doi.org/10.1016/j.bbamcr.2011.07.008
Boender LG, et al. Extreme Calorie Restriction and Energy Source Starvation in Saccharomyces Cerevisiae Represent Distinct Physiological States. Biochim Biophys Acta. 2011;1813(12):2133-44. PubMed PMID: 21803078.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extreme calorie restriction and energy source starvation in Saccharomyces cerevisiae represent distinct physiological states. AU - Boender,Léonie G M, AU - Almering,Marinka J H, AU - Dijk,Madelon, AU - van Maris,Antonius J A, AU - de Winde,Johannes H, AU - Pronk,Jack T, AU - Daran-Lapujade,Pascale, Y1 - 2011/07/22/ PY - 2011/04/05/received PY - 2011/07/07/revised PY - 2011/07/15/accepted PY - 2011/8/2/entrez PY - 2011/8/2/pubmed PY - 2013/3/21/medline SP - 2133 EP - 44 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1813 IS - 12 N2 - Cultivation methods used to investigate microbial calorie restriction often result in carbon and energy starvation. This study aims to dissect cellular responses to calorie restriction and starvation in Saccharomyces cerevisiae by using retentostat cultivation. In retentostats, cells are continuously supplied with a small, constant carbon and energy supply, sufficient for maintenance of cellular viability and integrity but insufficient for growth. When glucose-limited retentostats cultivated under extreme calorie restriction were subjected to glucose starvation, calorie-restricted and glucose-starved cells were found to share characteristics such as increased heat-shock tolerance and expression of quiescence-related genes. However, they also displayed strikingly different features. While calorie-restricted yeast cultures remained metabolically active and viable for prolonged periods of time, glucose starvation resulted in rapid consumption of reserve carbohydrates, population heterogeneity due to appearance of senescent cells and, ultimately, loss of viability. Moreover, during starvation, calculated rates of ATP synthesis from reserve carbohydrates were 2-3 orders of magnitude lower than steady-state ATP-turnover rates calculated under extreme calorie restriction in retentostats. Stringent reduction of ATP turnover during glucose starvation was accompanied by a strong down-regulation of genes involved in protein synthesis. These results demonstrate that extreme calorie restriction and carbon starvation represent different physiological states in S. cerevisiae. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/21803078/Extreme_calorie_restriction_and_energy_source_starvation_in_Saccharomyces_cerevisiae_represent_distinct_physiological_states_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-4889(11)00197-2 DB - PRIME DP - Unbound Medicine ER -