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Quercetin protects oligodendrocyte precursor cells from oxygen/glucose deprivation injury in vitro via the activation of the PI3K/Akt signaling pathway.
Brain Res Bull. 2011 Oct 10; 86(3-4):277-84.BR

Abstract

The aim of this study was to investigate the protection of quercetin (QUE) on oligodendrocyte precursor cells (OPCs) from oxygen/glucose deprivation (OGD)-induced injury in vitro and explore whether the PI3K/Akt signaling pathway contributed to the protection provided by quercetin. The OGD condition was induced by including 2mM sodium dithionite (Na(2)S(2)O(4)) in glucose-free DMEM medium. The concentration of QUE in this study ranged from 3μM to 81μM. OPCs were identified by immunocytochemical staining. Cell viability was analyzed using the water soluble tetrazolium salt-8 (WST-8) and lactate dehydrogenase assay (LDH). The morphological changes of the nucleus were measured using Hoechst 33258 nuclear staining, and the ratio of apoptotic cells was determined by FITC annexin V- and propidium iodide (PI) flow cytometry assay kit. In addition, the levels of pro-apoptotic proteins such as cleaved-caspase-3 and Bax and the anti-apoptotic proteins p-Akt and Bcl-2 were quantified using western blotting. The results showed that the OPC cell survival rate was significantly increased by incubation in conditioned medium supplemented with QUE as measured by the WST-8 assay, while the LDH release rate was significantly decreased as analyzed by the LDH assay. Furthermore, apoptosis assay showed that the apoptosis ratio of OPCs was also dramatically reduced by QUE. Western blotting showed that the expression levels of Bax and cleaved-caspase-3 proteins were down-regulated, while Bcl-2 and p-Akt were up-regulated. Further study showed that the increase in p-Akt by QUE was reduced by the PI3K inhibitor LY294002. These results indicated that QUE effectively protected OPCs from OGD-induced injury and that the mechanism might be related to the activation of the PI3K/Akt signaling pathway.

Authors+Show Affiliations

Department of Neurobiology, Xuzhou Medical College, Xuzhou, Jiangsu Province, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21803128

Citation

Wang, X-Q, et al. "Quercetin Protects Oligodendrocyte Precursor Cells From Oxygen/glucose Deprivation Injury in Vitro Via the Activation of the PI3K/Akt Signaling Pathway." Brain Research Bulletin, vol. 86, no. 3-4, 2011, pp. 277-84.
Wang XQ, Yao RQ, Liu X, et al. Quercetin protects oligodendrocyte precursor cells from oxygen/glucose deprivation injury in vitro via the activation of the PI3K/Akt signaling pathway. Brain Res Bull. 2011;86(3-4):277-84.
Wang, X. Q., Yao, R. Q., Liu, X., Huang, J. J., Qi, D. S., & Yang, L. H. (2011). Quercetin protects oligodendrocyte precursor cells from oxygen/glucose deprivation injury in vitro via the activation of the PI3K/Akt signaling pathway. Brain Research Bulletin, 86(3-4), 277-84. https://doi.org/10.1016/j.brainresbull.2011.07.014
Wang XQ, et al. Quercetin Protects Oligodendrocyte Precursor Cells From Oxygen/glucose Deprivation Injury in Vitro Via the Activation of the PI3K/Akt Signaling Pathway. Brain Res Bull. 2011 Oct 10;86(3-4):277-84. PubMed PMID: 21803128.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quercetin protects oligodendrocyte precursor cells from oxygen/glucose deprivation injury in vitro via the activation of the PI3K/Akt signaling pathway. AU - Wang,X-Q, AU - Yao,R-Q, AU - Liu,X, AU - Huang,J-J, AU - Qi,D-S, AU - Yang,L-H, Y1 - 2011/07/23/ PY - 2011/01/21/received PY - 2011/07/14/revised PY - 2011/07/15/accepted PY - 2011/8/2/entrez PY - 2011/8/2/pubmed PY - 2012/1/24/medline SP - 277 EP - 84 JF - Brain research bulletin JO - Brain Res. Bull. VL - 86 IS - 3-4 N2 - The aim of this study was to investigate the protection of quercetin (QUE) on oligodendrocyte precursor cells (OPCs) from oxygen/glucose deprivation (OGD)-induced injury in vitro and explore whether the PI3K/Akt signaling pathway contributed to the protection provided by quercetin. The OGD condition was induced by including 2mM sodium dithionite (Na(2)S(2)O(4)) in glucose-free DMEM medium. The concentration of QUE in this study ranged from 3μM to 81μM. OPCs were identified by immunocytochemical staining. Cell viability was analyzed using the water soluble tetrazolium salt-8 (WST-8) and lactate dehydrogenase assay (LDH). The morphological changes of the nucleus were measured using Hoechst 33258 nuclear staining, and the ratio of apoptotic cells was determined by FITC annexin V- and propidium iodide (PI) flow cytometry assay kit. In addition, the levels of pro-apoptotic proteins such as cleaved-caspase-3 and Bax and the anti-apoptotic proteins p-Akt and Bcl-2 were quantified using western blotting. The results showed that the OPC cell survival rate was significantly increased by incubation in conditioned medium supplemented with QUE as measured by the WST-8 assay, while the LDH release rate was significantly decreased as analyzed by the LDH assay. Furthermore, apoptosis assay showed that the apoptosis ratio of OPCs was also dramatically reduced by QUE. Western blotting showed that the expression levels of Bax and cleaved-caspase-3 proteins were down-regulated, while Bcl-2 and p-Akt were up-regulated. Further study showed that the increase in p-Akt by QUE was reduced by the PI3K inhibitor LY294002. These results indicated that QUE effectively protected OPCs from OGD-induced injury and that the mechanism might be related to the activation of the PI3K/Akt signaling pathway. SN - 1873-2747 UR - https://www.unboundmedicine.com/medline/citation/21803128/Quercetin_protects_oligodendrocyte_precursor_cells_from_oxygen/glucose_deprivation_injury_in_vitro_via_the_activation_of_the_PI3K/Akt_signaling_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0361-9230(11)00227-9 DB - PRIME DP - Unbound Medicine ER -