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Tri-enzyme functionalized ZnO-NPs/CHIT/c-MWCNT/PANI composite film for amperometric determination of creatinine.
Biosens Bioelectron. 2011 Oct 15; 28(1):64-70.BB

Abstract

A new zinc oxide nanoparticles/chitosan/carboxylated multiwall carbonnanotube/polyaniline (ZnO-NPs/CHIT/c-MWCNT/PANI) composite film has been synthesized on platinum (Pt) electrode using electrochemical techniques. Three enzymes, creatinine amidohydrolase (CA), creatine amidinohydrolase (CI) and sarcosine oxidase (SO) were immobilized on ZnO-NPs/CHIT/c-MWCNT/PANI/Pt electrode to construct the creatinine biosensor. The enzyme electrode was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS). The enzyme electrode detects creatinine level as low as 0.5 μM at a signal to noise ratio of 3 within 10s at pH 7.5 and 30°C. The fabricated creatinine biosensor showed linear working range of 10-650 μM creatinine with a sensitivity of 0.030 μA μM(-1)cm(-2). The biosensor shows only 15% loss of its initial response over a period of 120 days when stored at 4°C. The fabricated biosensor was successfully employed for determination of creatinine in human blood serum.

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Authors+Show Affiliations

Yadav S
Department of Biochemistry, Maharshi Dayanand University, Rohtak 124001, Haryana, India.
Devi R
No affiliation info available
Kumar A
No affiliation info available
Pundir CS
No affiliation info available

MeSH

Aniline CompoundsBiosensing TechniquesChitosanCreatinineDielectric SpectroscopyElectrochemical TechniquesNanoparticlesNanotubes, CarbonSpectroscopy, Fourier Transform InfraredZinc Oxide

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21803561

Citation

Yadav, Sandeep, et al. "Tri-enzyme Functionalized ZnO-NPs/CHIT/c-MWCNT/PANI Composite Film for Amperometric Determination of Creatinine." Biosensors & Bioelectronics, vol. 28, no. 1, 2011, pp. 64-70.
Yadav S, Devi R, Kumar A, et al. Tri-enzyme functionalized ZnO-NPs/CHIT/c-MWCNT/PANI composite film for amperometric determination of creatinine. Biosens Bioelectron. 2011;28(1):64-70.
Yadav, S., Devi, R., Kumar, A., & Pundir, C. S. (2011). Tri-enzyme functionalized ZnO-NPs/CHIT/c-MWCNT/PANI composite film for amperometric determination of creatinine. Biosensors & Bioelectronics, 28(1), 64-70. https://doi.org/10.1016/j.bios.2011.06.044
Yadav S, et al. Tri-enzyme Functionalized ZnO-NPs/CHIT/c-MWCNT/PANI Composite Film for Amperometric Determination of Creatinine. Biosens Bioelectron. 2011 Oct 15;28(1):64-70. PubMed PMID: 21803561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tri-enzyme functionalized ZnO-NPs/CHIT/c-MWCNT/PANI composite film for amperometric determination of creatinine. AU - Yadav,Sandeep, AU - Devi,Rooma, AU - Kumar,Ashok, AU - Pundir,C S, Y1 - 2011/07/06/ PY - 2011/04/15/received PY - 2011/06/22/revised PY - 2011/06/28/accepted PY - 2011/8/2/entrez PY - 2011/8/2/pubmed PY - 2012/1/24/medline SP - 64 EP - 70 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 28 IS - 1 N2 - A new zinc oxide nanoparticles/chitosan/carboxylated multiwall carbonnanotube/polyaniline (ZnO-NPs/CHIT/c-MWCNT/PANI) composite film has been synthesized on platinum (Pt) electrode using electrochemical techniques. Three enzymes, creatinine amidohydrolase (CA), creatine amidinohydrolase (CI) and sarcosine oxidase (SO) were immobilized on ZnO-NPs/CHIT/c-MWCNT/PANI/Pt electrode to construct the creatinine biosensor. The enzyme electrode was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS). The enzyme electrode detects creatinine level as low as 0.5 μM at a signal to noise ratio of 3 within 10s at pH 7.5 and 30°C. The fabricated creatinine biosensor showed linear working range of 10-650 μM creatinine with a sensitivity of 0.030 μA μM(-1)cm(-2). The biosensor shows only 15% loss of its initial response over a period of 120 days when stored at 4°C. The fabricated biosensor was successfully employed for determination of creatinine in human blood serum. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/21803561/Tri_enzyme_functionalized_ZnO_NPs/CHIT/c_MWCNT/PANI_composite_film_for_amperometric_determination_of_creatinine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(11)00408-8 DB - PRIME DP - Unbound Medicine ER -