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Lentiviral vector-mediated siRNA knockdown of the YAP gene inhibits growth and induces apoptosis in the SGC7901 gastric cancer cell line.
Mol Med Rep. 2011 Nov-Dec; 4(6):1075-82.MM

Abstract

Gastric carcinoma is among the most prevalent malignancies, and a leading cause of cancer deaths worldwide. The Hippo pathway defines a novel signaling pathway regulating cell proliferation. The key factor in this kinase cascade is the transcriptional co-activator yes-associated protein (YAP), which is constitutively activated in various types of cancer, including gastric carcinoma. To determine the effect on SGC7901 gastric cancer cells after inhibition of the YAP expression, we used lentivirus-derived small hairpin RNA (shRNA) against YAP. The YAP-specific shRNA significantly suppressed YAP expression at the mRNA and protein levels, reduced cancer cell proliferation and promoted cancer cell apoptosis. It was also found that YAP knockdown significantly increased the G0/G1 cell population and reduced expression levels of a number of genes crucial to cell proliferation and apoptosis, including Ki-67, proliferating cell nuclear antigen (PCNA), survivin and cyclin D1. Thus, our data suggested that knockdown of YAP expression plays a significant role in gastric cancer cell proliferation and apoptosis. Therefore, the YAP gene serves as a potential therapeutic target in gastric cancers.

Authors+Show Affiliations

Department of Gastroenterology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, PR China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21805037

Citation

Zhou, Zhou, et al. "Lentiviral Vector-mediated siRNA Knockdown of the YAP Gene Inhibits Growth and Induces Apoptosis in the SGC7901 Gastric Cancer Cell Line." Molecular Medicine Reports, vol. 4, no. 6, 2011, pp. 1075-82.
Zhou Z, Zhu JS, Xu ZP, et al. Lentiviral vector-mediated siRNA knockdown of the YAP gene inhibits growth and induces apoptosis in the SGC7901 gastric cancer cell line. Mol Med Rep. 2011;4(6):1075-82.
Zhou, Z., Zhu, J. S., Xu, Z. P., & Zhang, Q. (2011). Lentiviral vector-mediated siRNA knockdown of the YAP gene inhibits growth and induces apoptosis in the SGC7901 gastric cancer cell line. Molecular Medicine Reports, 4(6), 1075-82. https://doi.org/10.3892/mmr.2011.543
Zhou Z, et al. Lentiviral Vector-mediated siRNA Knockdown of the YAP Gene Inhibits Growth and Induces Apoptosis in the SGC7901 Gastric Cancer Cell Line. Mol Med Rep. 2011 Nov-Dec;4(6):1075-82. PubMed PMID: 21805037.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lentiviral vector-mediated siRNA knockdown of the YAP gene inhibits growth and induces apoptosis in the SGC7901 gastric cancer cell line. AU - Zhou,Zhou, AU - Zhu,Jin-Shui, AU - Xu,Zhi-Peng, AU - Zhang,Qiang, Y1 - 2011/07/28/ PY - 2011/03/23/received PY - 2011/07/22/accepted PY - 2011/8/2/entrez PY - 2011/8/2/pubmed PY - 2012/1/10/medline SP - 1075 EP - 82 JF - Molecular medicine reports JO - Mol Med Rep VL - 4 IS - 6 N2 - Gastric carcinoma is among the most prevalent malignancies, and a leading cause of cancer deaths worldwide. The Hippo pathway defines a novel signaling pathway regulating cell proliferation. The key factor in this kinase cascade is the transcriptional co-activator yes-associated protein (YAP), which is constitutively activated in various types of cancer, including gastric carcinoma. To determine the effect on SGC7901 gastric cancer cells after inhibition of the YAP expression, we used lentivirus-derived small hairpin RNA (shRNA) against YAP. The YAP-specific shRNA significantly suppressed YAP expression at the mRNA and protein levels, reduced cancer cell proliferation and promoted cancer cell apoptosis. It was also found that YAP knockdown significantly increased the G0/G1 cell population and reduced expression levels of a number of genes crucial to cell proliferation and apoptosis, including Ki-67, proliferating cell nuclear antigen (PCNA), survivin and cyclin D1. Thus, our data suggested that knockdown of YAP expression plays a significant role in gastric cancer cell proliferation and apoptosis. Therefore, the YAP gene serves as a potential therapeutic target in gastric cancers. SN - 1791-3004 UR - https://www.unboundmedicine.com/medline/citation/21805037/Lentiviral_vector_mediated_siRNA_knockdown_of_the_YAP_gene_inhibits_growth_and_induces_apoptosis_in_the_SGC7901_gastric_cancer_cell_line_ L2 - http://www.spandidos-publications.com/mmr/4/6/1075 DB - PRIME DP - Unbound Medicine ER -