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Antioxidant activity and protective effect against plasmid DNA strand scission of leaf, bark, and heartwood extracts from Acacia catechu.
J Food Sci. 2011 Sep; 76(7):C959-64.JF

Abstract

The antioxidant activity of methanol extract/fractions of leaf, bark, and heartwood of Acacia catechu was evaluated by various antioxidant assays, including free radical, superoxide and hydroxyl radical, reducing power, metal ion chelation, as well as hydroxyl radical induced DNA strand scission. The leaf, bark, and heartwood powder was extracted in methanol and the lyophilized methanol extract was fractionated with different solvents in the order of increasing polarity. The results indicate that ethyl acetate fraction of heartwood has the highest antioxidant capacities, presenting lower EC(50) values particularly in free radical scavenging activity, including DPPH radicals (4.76 ± 0.14 μg/mL), superoxide anions (26.21 ± 0.79 μg/mL), and hydroxyl radicals (33.69 ± 1.42 μg/mL), in direct assay systems. Reducing power was also highest in ethyl acetate fraction of heartwood (EC(50) of 79.05 ± 1.02 μg/mL). As for the chelating power on ferrous ions, leaf extract was more effective than bark and heartwood extracts. Furthermore, the ethyl acetate and acetone fractions of heartwood significantly protected pBR322 supercoiled plasmid DNA against strand scission induced by hydroxyl radicals in a Fenton's reaction mixture.

PRACTICAL APPLICATION

The present investigation suggests that the three organs of A. catechu differ significantly in their antioxidant potential as seen in the DPPH radical scavenging assay, reducing power assay, metal ion chelating assay, superoxide radical scavenging assay and hydroxyl radical scavenging assay. Further, our results showed that crude methanol extract and ethyl acetate fraction of heartwood of A. catechu might have a good potential as a source for natural health products due to its antioxidant and DNA protective activities.

Authors+Show Affiliations

Div of Biochemistry and Plant Physiology, SK Univ of Agricultural Sciences and Technology, Chatha 180 009, Jammu, India. guleria71@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21806606

Citation

Guleria, Sanjay, et al. "Antioxidant Activity and Protective Effect Against Plasmid DNA Strand Scission of Leaf, Bark, and Heartwood Extracts From Acacia Catechu." Journal of Food Science, vol. 76, no. 7, 2011, pp. C959-64.
Guleria S, Tiku AK, Singh G, et al. Antioxidant activity and protective effect against plasmid DNA strand scission of leaf, bark, and heartwood extracts from Acacia catechu. J Food Sci. 2011;76(7):C959-64.
Guleria, S., Tiku, A. K., Singh, G., Vyas, D., & Bhardwaj, A. (2011). Antioxidant activity and protective effect against plasmid DNA strand scission of leaf, bark, and heartwood extracts from Acacia catechu. Journal of Food Science, 76(7), C959-64. https://doi.org/10.1111/j.1750-3841.2011.02284.x
Guleria S, et al. Antioxidant Activity and Protective Effect Against Plasmid DNA Strand Scission of Leaf, Bark, and Heartwood Extracts From Acacia Catechu. J Food Sci. 2011;76(7):C959-64. PubMed PMID: 21806606.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant activity and protective effect against plasmid DNA strand scission of leaf, bark, and heartwood extracts from Acacia catechu. AU - Guleria,Sanjay, AU - Tiku,A K, AU - Singh,Gurjinder, AU - Vyas,Dhiraj, AU - Bhardwaj,Ashwani, Y1 - 2011/08/01/ PY - 2011/8/3/entrez PY - 2011/8/3/pubmed PY - 2012/7/14/medline SP - C959 EP - 64 JF - Journal of food science JO - J Food Sci VL - 76 IS - 7 N2 - UNLABELLED: The antioxidant activity of methanol extract/fractions of leaf, bark, and heartwood of Acacia catechu was evaluated by various antioxidant assays, including free radical, superoxide and hydroxyl radical, reducing power, metal ion chelation, as well as hydroxyl radical induced DNA strand scission. The leaf, bark, and heartwood powder was extracted in methanol and the lyophilized methanol extract was fractionated with different solvents in the order of increasing polarity. The results indicate that ethyl acetate fraction of heartwood has the highest antioxidant capacities, presenting lower EC(50) values particularly in free radical scavenging activity, including DPPH radicals (4.76 ± 0.14 μg/mL), superoxide anions (26.21 ± 0.79 μg/mL), and hydroxyl radicals (33.69 ± 1.42 μg/mL), in direct assay systems. Reducing power was also highest in ethyl acetate fraction of heartwood (EC(50) of 79.05 ± 1.02 μg/mL). As for the chelating power on ferrous ions, leaf extract was more effective than bark and heartwood extracts. Furthermore, the ethyl acetate and acetone fractions of heartwood significantly protected pBR322 supercoiled plasmid DNA against strand scission induced by hydroxyl radicals in a Fenton's reaction mixture. PRACTICAL APPLICATION: The present investigation suggests that the three organs of A. catechu differ significantly in their antioxidant potential as seen in the DPPH radical scavenging assay, reducing power assay, metal ion chelating assay, superoxide radical scavenging assay and hydroxyl radical scavenging assay. Further, our results showed that crude methanol extract and ethyl acetate fraction of heartwood of A. catechu might have a good potential as a source for natural health products due to its antioxidant and DNA protective activities. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/21806606/Antioxidant_activity_and_protective_effect_against_plasmid_DNA_strand_scission_of_leaf_bark_and_heartwood_extracts_from_Acacia_catechu_ L2 - https://doi.org/10.1111/j.1750-3841.2011.02284.x DB - PRIME DP - Unbound Medicine ER -