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The antimitogenic effect of the cannabinoid receptor agonist WIN55212-2 on human melanoma cells is mediated by the membrane lipid raft.

Abstract

Here are reported the antiproliferative effects of the cannabinoid agonist WIN upon human melanoma cells expressing mRNA and protein for both CB1 and CB2 receptors. While WIN exerted antimitogenic effects, selective CB1 or CB2 agonists were unable to reproduce such effects and selective CB1 and CB2 antagonists did not inhibit WIN-induced cell death. Cells treated with WIN, preincubated with the lipid raft disruptor methylcyclodestrin, were rescued from death. WIN induced activation of caspases and phosphorylation of ERK that were attenuated in cultures treated with methylcyclodestrin. Membrane lipid raft complex-mediated antimitogenic effect of WIN in melanoma could represents a potential targets for a melanoma treatment.

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  • Authors+Show Affiliations

    ,

    Department of Clinical and Molecular Biomedicine, University of Catania School of Medicine, Catania, Italy.

    , , , , ,

    Source

    Cancer letters 310:2 2011 Nov 28 pg 240-9

    MeSH

    Apoptosis
    Benzoxazines
    Cannabinoids
    Cell Death
    Cell Line, Tumor
    Cell Survival
    Humans
    Melanoma
    Membrane Microdomains
    Morpholines
    Naphthalenes
    Receptor, Cannabinoid, CB1
    Receptor, Cannabinoid, CB2
    Signal Transduction

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    21807457

    Citation

    Scuderi, Mariagrazia Rita, et al. "The Antimitogenic Effect of the Cannabinoid Receptor Agonist WIN55212-2 On Human Melanoma Cells Is Mediated By the Membrane Lipid Raft." Cancer Letters, vol. 310, no. 2, 2011, pp. 240-9.
    Scuderi MR, Cantarella G, Scollo M, et al. The antimitogenic effect of the cannabinoid receptor agonist WIN55212-2 on human melanoma cells is mediated by the membrane lipid raft. Cancer Lett. 2011;310(2):240-9.
    Scuderi, M. R., Cantarella, G., Scollo, M., Lempereur, L., Palumbo, M., Saccani-Jotti, G., & Bernardini, R. (2011). The antimitogenic effect of the cannabinoid receptor agonist WIN55212-2 on human melanoma cells is mediated by the membrane lipid raft. Cancer Letters, 310(2), pp. 240-9. doi:10.1016/j.canlet.2011.07.008.
    Scuderi MR, et al. The Antimitogenic Effect of the Cannabinoid Receptor Agonist WIN55212-2 On Human Melanoma Cells Is Mediated By the Membrane Lipid Raft. Cancer Lett. 2011 Nov 28;310(2):240-9. PubMed PMID: 21807457.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The antimitogenic effect of the cannabinoid receptor agonist WIN55212-2 on human melanoma cells is mediated by the membrane lipid raft. AU - Scuderi,Mariagrazia Rita, AU - Cantarella,Giuseppina, AU - Scollo,Mimmo, AU - Lempereur,Laurence, AU - Palumbo,Marco, AU - Saccani-Jotti,Gloria, AU - Bernardini,Renato, Y1 - 2011/07/19/ PY - 2011/06/21/received PY - 2011/07/06/revised PY - 2011/07/07/accepted PY - 2011/8/3/entrez PY - 2011/8/3/pubmed PY - 2011/11/16/medline SP - 240 EP - 9 JF - Cancer letters JO - Cancer Lett. VL - 310 IS - 2 N2 - Here are reported the antiproliferative effects of the cannabinoid agonist WIN upon human melanoma cells expressing mRNA and protein for both CB1 and CB2 receptors. While WIN exerted antimitogenic effects, selective CB1 or CB2 agonists were unable to reproduce such effects and selective CB1 and CB2 antagonists did not inhibit WIN-induced cell death. Cells treated with WIN, preincubated with the lipid raft disruptor methylcyclodestrin, were rescued from death. WIN induced activation of caspases and phosphorylation of ERK that were attenuated in cultures treated with methylcyclodestrin. Membrane lipid raft complex-mediated antimitogenic effect of WIN in melanoma could represents a potential targets for a melanoma treatment. SN - 1872-7980 UR - https://www.unboundmedicine.com/medline/citation/21807457/The_antimitogenic_effect_of_the_cannabinoid_receptor_agonist_WIN55212_2_on_human_melanoma_cells_is_mediated_by_the_membrane_lipid_raft_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3835(11)00404-6 DB - PRIME DP - Unbound Medicine ER -