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[Study on the quality and genetic diversity of Gentiana macrophylla pall of different habitats].
Zhong Yao Cai. 2011 Apr; 34(4):517-9.ZY

Abstract

OBJECTIVE

To study the quality and genetic diversity in Gentiana macrophylla of different habitats for controlling the quality of Gentiana macrophylla.

METHODS

Main characteristics and microscopic identification were adapted to identify Gentiana macrophylla; HPLC method was applied for analysising the contents of Gentiana macrophylla. RAPD method was applied to study genetic structure and genetic diversity of Gentiana macrophylla.

RESULTS

The experiment showed that the Gentiana macrophylla of different habitats have different main charactericstics, microscopic identifications and contents, and effective ingredient contents of gentiopicroside. The levels of population genetic diversity of various groups followed by HUANGZHONG County population (K) > HUAN County population (J) > ZiWU Mountain population (H).

CONCLUSION

The study of external morphology, internal structure, active ingredient content and genetic level of Gentiana macrophylla from different areas provides a scientific basis for protecting wild species and ensuring the quality in introducing and cultivating Gentiana macrophylla.

Authors+Show Affiliations

Wu YH
Gansu College of TCM, Lanzhou 730000, China. yhw67@126.com
Di W
No affiliation info available
Cui ZJ
No affiliation info available
Li YF
No affiliation info available
Liu LS
No affiliation info available

MeSH

Chromatography, High Pressure LiquidGenetic VariationGentianaIridoid GlucosidesPlant RootsPlants, MedicinalQuality ControlRandom Amplified Polymorphic DNA TechniqueSpecies Specificity

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21809537

Citation

Wu, Yu-hong, et al. "[Study On the Quality and Genetic Diversity of Gentiana Macrophylla Pall of Different Habitats]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 34, no. 4, 2011, pp. 517-9.
Wu YH, Di W, Cui ZJ, et al. [Study on the quality and genetic diversity of Gentiana macrophylla pall of different habitats]. Zhong Yao Cai. 2011;34(4):517-9.
Wu, Y. H., Di, W., Cui, Z. J., Li, Y. F., & Liu, L. S. (2011). [Study on the quality and genetic diversity of Gentiana macrophylla pall of different habitats]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 34(4), 517-9.
Wu YH, et al. [Study On the Quality and Genetic Diversity of Gentiana Macrophylla Pall of Different Habitats]. Zhong Yao Cai. 2011;34(4):517-9. PubMed PMID: 21809537.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on the quality and genetic diversity of Gentiana macrophylla pall of different habitats]. AU - Wu,Yu-hong, AU - Di,Wu, AU - Cui,Zhi-jia, AU - Li,Yue-feng, AU - Liu,Li-sha, PY - 2011/8/4/entrez PY - 2011/8/4/pubmed PY - 2012/1/20/medline SP - 517 EP - 9 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 34 IS - 4 N2 - OBJECTIVE: To study the quality and genetic diversity in Gentiana macrophylla of different habitats for controlling the quality of Gentiana macrophylla. METHODS: Main characteristics and microscopic identification were adapted to identify Gentiana macrophylla; HPLC method was applied for analysising the contents of Gentiana macrophylla. RAPD method was applied to study genetic structure and genetic diversity of Gentiana macrophylla. RESULTS: The experiment showed that the Gentiana macrophylla of different habitats have different main charactericstics, microscopic identifications and contents, and effective ingredient contents of gentiopicroside. The levels of population genetic diversity of various groups followed by HUANGZHONG County population (K) > HUAN County population (J) > ZiWU Mountain population (H). CONCLUSION: The study of external morphology, internal structure, active ingredient content and genetic level of Gentiana macrophylla from different areas provides a scientific basis for protecting wild species and ensuring the quality in introducing and cultivating Gentiana macrophylla. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/21809537/[Study_on_the_quality_and_genetic_diversity_of_Gentiana_macrophylla_pall_of_different_habitats]_ DB - PRIME DP - Unbound Medicine ER -