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[Determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride in maxingshigan decoction by CE].
Zhong Yao Cai. 2011 Apr; 34(4):620-3.ZY

Abstract

OBJECTIVE

To establish the method for determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride in maxingshigan decoction by capillary electrophoresis.

METHODS

The separation was performed on a fused silica capillary of 60 cm x 55 microcrpm ID (52 cm of effective length). 60 mmol/L NaB4O7 + 10% (V/V) CH3OH (pH 9.0) was selected as the running buffer. The separation voltage was 12 kV. The samples was injected by gravity (10 s, 15 cm). The detection wavelength was 210 nm and berberine hydrochloride was the internal standard.

RESULTS

The linear range of determination for ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride were 20.0-160.0 microg/mL (r = 0.9999), 7.5-60.0 microg/mL (r = 0.9991) and 2.0-10.0 microg/mL (r = 0.9993). The average recoveries were 98.0%, 97.0% and 97.8%, the precisions of the method were 2.31%, 2.21% and 2.00% (n=6), respectively.

CONCLUSION

The method is convenient, rapid and accurate for the quality control of maxingshigan decoction.

Authors+Show Affiliations

School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. aleckyu@126.comNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21809550

Citation

Yu, Li-ping, et al. "[Determination of Ephedrine Hydrochloride, Pseudoephedrine Hydrochloride and Methylephedrine Hydrochloride in Maxingshigan Decoction By CE]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 34, no. 4, 2011, pp. 620-3.
Yu LP, Wang XK, Luo JB. [Determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride in maxingshigan decoction by CE]. Zhong Yao Cai. 2011;34(4):620-3.
Yu, L. P., Wang, X. K., & Luo, J. B. (2011). [Determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride in maxingshigan decoction by CE]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 34(4), 620-3.
Yu LP, Wang XK, Luo JB. [Determination of Ephedrine Hydrochloride, Pseudoephedrine Hydrochloride and Methylephedrine Hydrochloride in Maxingshigan Decoction By CE]. Zhong Yao Cai. 2011;34(4):620-3. PubMed PMID: 21809550.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride in maxingshigan decoction by CE]. AU - Yu,Li-ping, AU - Wang,Xiao-ke, AU - Luo,Jia-bo, PY - 2011/8/4/entrez PY - 2011/8/4/pubmed PY - 2012/1/20/medline SP - 620 EP - 3 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 34 IS - 4 N2 - OBJECTIVE: To establish the method for determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride in maxingshigan decoction by capillary electrophoresis. METHODS: The separation was performed on a fused silica capillary of 60 cm x 55 microcrpm ID (52 cm of effective length). 60 mmol/L NaB4O7 + 10% (V/V) CH3OH (pH 9.0) was selected as the running buffer. The separation voltage was 12 kV. The samples was injected by gravity (10 s, 15 cm). The detection wavelength was 210 nm and berberine hydrochloride was the internal standard. RESULTS: The linear range of determination for ephedrine hydrochloride, pseudoephedrine hydrochloride and methylephedrine hydrochloride were 20.0-160.0 microg/mL (r = 0.9999), 7.5-60.0 microg/mL (r = 0.9991) and 2.0-10.0 microg/mL (r = 0.9993). The average recoveries were 98.0%, 97.0% and 97.8%, the precisions of the method were 2.31%, 2.21% and 2.00% (n=6), respectively. CONCLUSION: The method is convenient, rapid and accurate for the quality control of maxingshigan decoction. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/21809550/[Determination_of_ephedrine_hydrochloride_pseudoephedrine_hydrochloride_and_methylephedrine_hydrochloride_in_maxingshigan_decoction_by_CE]_ DB - PRIME DP - Unbound Medicine ER -