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Elevated serum osteopontin levels and genetic polymorphisms of osteopontin are associated with Vogt-Koyanagi-Harada disease.
Invest Ophthalmol Vis Sci. 2011 Sep 09; 52(10):7084-9.IO

Abstract

PURPOSE

Osteopontin (OPN) is a proinflammatory cytokine involved in chronic inflammatory diseases. This study aimed to analyze the role of OPN in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease.

METHODS

Serum levels of OPN in VKH patients and healthy controls were assayed by enzyme-linked immunosorbent assay (ELISA). Peripheral blood mononuclear cells (PBMCs) or CD4+ T cells were cultured with anti-CD3 and anti-CD28 antibodies in the absence or presence of recombinant OPN for the determination of cell proliferation and cytokines. Cell proliferation was detected using a cell counting kit. Levels of interferon (IFN)-γ and interleukin (IL)-17 were detected by ELISA. Four single nucleotide polymorphisms (SNPs) of OPN and four SNPs of OPN receptors were genotyped in 601 VKH patients and 605 healthy controls using a polymerase chain reaction-restriction fragment length polymorphism assay.

RESULTS

OPN serum levels were significantly higher in patients with active VKH than in patients with inactive VKH and in healthy controls. PBMCs or CD4+ T cells cultured with recombinant OPN induced a marked cell proliferation and profound secretion of IFN-γ and IL-17 from patients with active VKH. A significantly increased frequency of the OPN rs4754 TT genotype (P = 0.004, pc = 0.048) was observed in VKH patients compared with healthy controls. No association could be detected among the four selected SNPs of OPN receptors and VKH.

CONCLUSIONS

OPN may be relevant to the pathogenesis of VKH disease. The TT genotype of rs4754 may be a susceptible factor for VKH disease in a Chinese Han population.

Authors+Show Affiliations

First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology and Chongqing Eye Institute, Chongqing, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21810982

Citation

Chu, Mingliang, et al. "Elevated Serum Osteopontin Levels and Genetic Polymorphisms of Osteopontin Are Associated With Vogt-Koyanagi-Harada Disease." Investigative Ophthalmology & Visual Science, vol. 52, no. 10, 2011, pp. 7084-9.
Chu M, Yang P, Hu R, et al. Elevated serum osteopontin levels and genetic polymorphisms of osteopontin are associated with Vogt-Koyanagi-Harada disease. Invest Ophthalmol Vis Sci. 2011;52(10):7084-9.
Chu, M., Yang, P., Hu, R., Hou, S., Li, F., Chen, Y., & Kijlstra, A. (2011). Elevated serum osteopontin levels and genetic polymorphisms of osteopontin are associated with Vogt-Koyanagi-Harada disease. Investigative Ophthalmology & Visual Science, 52(10), 7084-9. https://doi.org/10.1167/iovs.11-7539
Chu M, et al. Elevated Serum Osteopontin Levels and Genetic Polymorphisms of Osteopontin Are Associated With Vogt-Koyanagi-Harada Disease. Invest Ophthalmol Vis Sci. 2011 Sep 9;52(10):7084-9. PubMed PMID: 21810982.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elevated serum osteopontin levels and genetic polymorphisms of osteopontin are associated with Vogt-Koyanagi-Harada disease. AU - Chu,Mingliang, AU - Yang,Peizeng, AU - Hu,Ranran, AU - Hou,Shengping, AU - Li,Fuzhen, AU - Chen,Yuanyuan, AU - Kijlstra,Aize, Y1 - 2011/09/09/ PY - 2011/8/4/entrez PY - 2011/8/4/pubmed PY - 2011/11/5/medline SP - 7084 EP - 9 JF - Investigative ophthalmology & visual science JO - Invest. Ophthalmol. Vis. Sci. VL - 52 IS - 10 N2 - PURPOSE: Osteopontin (OPN) is a proinflammatory cytokine involved in chronic inflammatory diseases. This study aimed to analyze the role of OPN in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. METHODS: Serum levels of OPN in VKH patients and healthy controls were assayed by enzyme-linked immunosorbent assay (ELISA). Peripheral blood mononuclear cells (PBMCs) or CD4+ T cells were cultured with anti-CD3 and anti-CD28 antibodies in the absence or presence of recombinant OPN for the determination of cell proliferation and cytokines. Cell proliferation was detected using a cell counting kit. Levels of interferon (IFN)-γ and interleukin (IL)-17 were detected by ELISA. Four single nucleotide polymorphisms (SNPs) of OPN and four SNPs of OPN receptors were genotyped in 601 VKH patients and 605 healthy controls using a polymerase chain reaction-restriction fragment length polymorphism assay. RESULTS: OPN serum levels were significantly higher in patients with active VKH than in patients with inactive VKH and in healthy controls. PBMCs or CD4+ T cells cultured with recombinant OPN induced a marked cell proliferation and profound secretion of IFN-γ and IL-17 from patients with active VKH. A significantly increased frequency of the OPN rs4754 TT genotype (P = 0.004, pc = 0.048) was observed in VKH patients compared with healthy controls. No association could be detected among the four selected SNPs of OPN receptors and VKH. CONCLUSIONS: OPN may be relevant to the pathogenesis of VKH disease. The TT genotype of rs4754 may be a susceptible factor for VKH disease in a Chinese Han population. SN - 1552-5783 UR - https://www.unboundmedicine.com/medline/citation/21810982/Elevated_serum_osteopontin_levels_and_genetic_polymorphisms_of_osteopontin_are_associated_with_Vogt_Koyanagi_Harada_disease_ L2 - https://iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.11-7539 DB - PRIME DP - Unbound Medicine ER -