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Induced autophagy reduces virus output in dengue infected monocytic cells.
Virology. 2011 Sep 15; 418(1):74-84.V

Abstract

While several studies have shown a role for autophagy in the replication of dengue virus (DENV), these studies have been performed in directly infected cells. However, in severe cases of DENV infection the critical cell in the disease is believed to be monocytes which are poorly infected directly, but are highly susceptible to antibody enhanced infection. This study sought to determine the involvement of autophagy in the DENV infection of monocytic cells, using U937 cells as a model system. While the induction of autophagy was seen in response to DENV-2 infection, biochemical induction of autophagy resulted in a significant decrease in virus output. Down regulation of autophagy resulted in only a very slight increase in intracellular virus levels. In monocytic cells autophagy is not a significant part of the DENV replication mechanism, and there are distinct cell type specific differences in the DENV-autophagy interaction.

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Authors+Show Affiliations

Panyasrivanit M
Molecular Pathology Laboratory, Institute of Molecular Biosciences, Mahidol University, Thailand.
Greenwood MP
No affiliation info available
Murphy D
No affiliation info available
Isidoro C
No affiliation info available
Auewarakul P
No affiliation info available
Smith DR
No affiliation info available

MeSH

AutophagyCell LineDengue VirusHumansMonocytesViral LoadVirus CultivationVirus Replication

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21813150

Citation

Panyasrivanit, Mingkwan, et al. "Induced Autophagy Reduces Virus Output in Dengue Infected Monocytic Cells." Virology, vol. 418, no. 1, 2011, pp. 74-84.
Panyasrivanit M, Greenwood MP, Murphy D, et al. Induced autophagy reduces virus output in dengue infected monocytic cells. Virology. 2011;418(1):74-84.
Panyasrivanit, M., Greenwood, M. P., Murphy, D., Isidoro, C., Auewarakul, P., & Smith, D. R. (2011). Induced autophagy reduces virus output in dengue infected monocytic cells. Virology, 418(1), 74-84. https://doi.org/10.1016/j.virol.2011.07.010
Panyasrivanit M, et al. Induced Autophagy Reduces Virus Output in Dengue Infected Monocytic Cells. Virology. 2011 Sep 15;418(1):74-84. PubMed PMID: 21813150.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induced autophagy reduces virus output in dengue infected monocytic cells. AU - Panyasrivanit,Mingkwan, AU - Greenwood,Michael P, AU - Murphy,David, AU - Isidoro,Ciro, AU - Auewarakul,Prasert, AU - Smith,Duncan R, Y1 - 2011/08/02/ PY - 2011/06/08/received PY - 2011/07/03/revised PY - 2011/07/13/accepted PY - 2011/8/5/entrez PY - 2011/8/5/pubmed PY - 2011/10/19/medline SP - 74 EP - 84 JF - Virology JO - Virology VL - 418 IS - 1 N2 - While several studies have shown a role for autophagy in the replication of dengue virus (DENV), these studies have been performed in directly infected cells. However, in severe cases of DENV infection the critical cell in the disease is believed to be monocytes which are poorly infected directly, but are highly susceptible to antibody enhanced infection. This study sought to determine the involvement of autophagy in the DENV infection of monocytic cells, using U937 cells as a model system. While the induction of autophagy was seen in response to DENV-2 infection, biochemical induction of autophagy resulted in a significant decrease in virus output. Down regulation of autophagy resulted in only a very slight increase in intracellular virus levels. In monocytic cells autophagy is not a significant part of the DENV replication mechanism, and there are distinct cell type specific differences in the DENV-autophagy interaction. SN - 1096-0341 UR - https://www.unboundmedicine.com/medline/citation/21813150/Induced_autophagy_reduces_virus_output_in_dengue_infected_monocytic_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(11)00316-3 DB - PRIME DP - Unbound Medicine ER -