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Memory T-cell immune response in healthy young adults vaccinated with live attenuated influenza A (H5N2) vaccine.
Clin Vaccine Immunol. 2011 Oct; 18(10):1710-8.CV

Abstract

Cellular immune responses of both CD4 and CD8 memory/effector T cells were evaluated in healthy young adults who received two doses of live attenuated influenza A (H5N2) vaccine. The vaccine was developed by reassortment of nonpathogenic avian A/Duck/Potsdam/1402-6/68 (H5N2) and cold-adapted A/Leningrad/134/17/57 (H2N2) viruses. T-cell responses were measured by standard methods of intracellular cytokine staining of gamma interferon (IFN-γ)-producing cells and a novel T-cell recognition of antigen-presenting cells by protein capture (TRAP) assay based on the trogocytosis phenomenon, namely, plasma membrane exchange between interacting immune cells. TRAP enables the detection of activated trogocytosis-positive T cells after virus stimulation. We showed that two doses of live attenuated influenza A (H5N2) vaccine promoted both CD4 and CD8 T-memory-cell responses in peripheral blood of healthy young subjects in the clinical study. Significant differences in geometric mean titers (GMTs) of influenza A (H5N2)-specific IFN-γ(+) cells were observed at day 42 following the second vaccination, while peak levels of trogocytosis(+) T cells were detected earlier, on the 21st day after the second vaccination. The inverse correlation of baseline levels compared to postvaccine fold changes in GMTs of influenza-specific CD4 and CD8 T cells demonstrated that baseline levels of these specific cells could be considered a predictive factor of vaccine immunogenicity.

Authors+Show Affiliations

Department of Virology, Institute of Experimental Medicine, Acad. Pavlov St. 12, Saint Petersburg 197376, Russia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21813657

Citation

Chirkova, T V., et al. "Memory T-cell Immune Response in Healthy Young Adults Vaccinated With Live Attenuated Influenza a (H5N2) Vaccine." Clinical and Vaccine Immunology : CVI, vol. 18, no. 10, 2011, pp. 1710-8.
Chirkova TV, Naykhin AN, Petukhova GD, et al. Memory T-cell immune response in healthy young adults vaccinated with live attenuated influenza A (H5N2) vaccine. Clin Vaccine Immunol. 2011;18(10):1710-8.
Chirkova, T. V., Naykhin, A. N., Petukhova, G. D., Korenkov, D. A., Donina, S. A., Mironov, A. N., & Rudenko, L. G. (2011). Memory T-cell immune response in healthy young adults vaccinated with live attenuated influenza A (H5N2) vaccine. Clinical and Vaccine Immunology : CVI, 18(10), 1710-8. https://doi.org/10.1128/CVI.05116-11
Chirkova TV, et al. Memory T-cell Immune Response in Healthy Young Adults Vaccinated With Live Attenuated Influenza a (H5N2) Vaccine. Clin Vaccine Immunol. 2011;18(10):1710-8. PubMed PMID: 21813657.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Memory T-cell immune response in healthy young adults vaccinated with live attenuated influenza A (H5N2) vaccine. AU - Chirkova,T V, AU - Naykhin,A N, AU - Petukhova,G D, AU - Korenkov,D A, AU - Donina,S A, AU - Mironov,A N, AU - Rudenko,L G, Y1 - 2011/08/03/ PY - 2011/8/5/entrez PY - 2011/8/5/pubmed PY - 2012/1/24/medline SP - 1710 EP - 8 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 18 IS - 10 N2 - Cellular immune responses of both CD4 and CD8 memory/effector T cells were evaluated in healthy young adults who received two doses of live attenuated influenza A (H5N2) vaccine. The vaccine was developed by reassortment of nonpathogenic avian A/Duck/Potsdam/1402-6/68 (H5N2) and cold-adapted A/Leningrad/134/17/57 (H2N2) viruses. T-cell responses were measured by standard methods of intracellular cytokine staining of gamma interferon (IFN-γ)-producing cells and a novel T-cell recognition of antigen-presenting cells by protein capture (TRAP) assay based on the trogocytosis phenomenon, namely, plasma membrane exchange between interacting immune cells. TRAP enables the detection of activated trogocytosis-positive T cells after virus stimulation. We showed that two doses of live attenuated influenza A (H5N2) vaccine promoted both CD4 and CD8 T-memory-cell responses in peripheral blood of healthy young subjects in the clinical study. Significant differences in geometric mean titers (GMTs) of influenza A (H5N2)-specific IFN-γ(+) cells were observed at day 42 following the second vaccination, while peak levels of trogocytosis(+) T cells were detected earlier, on the 21st day after the second vaccination. The inverse correlation of baseline levels compared to postvaccine fold changes in GMTs of influenza-specific CD4 and CD8 T cells demonstrated that baseline levels of these specific cells could be considered a predictive factor of vaccine immunogenicity. SN - 1556-679X UR - https://www.unboundmedicine.com/medline/citation/21813657/Memory_T_cell_immune_response_in_healthy_young_adults_vaccinated_with_live_attenuated_influenza_A__H5N2__vaccine_ L2 - http://cvi.asm.org/cgi/pmidlookup?view=long&pmid=21813657 DB - PRIME DP - Unbound Medicine ER -