Tags

Type your tag names separated by a space and hit enter

Poly-N-acetyl glucosamine fibers are synergistic with vacuum-assisted closure in augmenting the healing response of diabetic mice.
J Trauma 2011; 71(2 Suppl 1):S187-93JT

Abstract

BACKGROUND

Vacuum-assisted closure (VAC) has become the preferred modality to treat many complex wounds but could be further improved by methods that minimize bleeding and facilitate wound epithelialization. Short fiber poly-N-acetyl glucosamine nanofibers (sNAG) are effective hemostatic agents that activate platelets and facilitate wound epithelialization. We hypothesized that sNAG used in combination with the VAC device could be synergistic in promoting wound healing while minimizing the risk of bleeding.

METHODS

Membranes consisting entirely of sNAG nanofibers were applied immediately to dorsal excisional wounds of db/db mice followed by application of the VAC device. Wound healing kinetics, angiogenesis, and wound-related growth factor expression were measured.

RESULTS

The application of sNAG membranes to wounds 24 hours before application of the VAC device was associated with a significant activation of wounds (expression of PDGF, TGFβ, EGF), superior granulation tissue formation rich in Collagen I as well as superior wound epithelialization (8.6% ± 0.3% vs. 1.8% ± 1.1% of initial wound size) and wound contraction.

CONCLUSIONS

The application of sNAG fiber-containing membranes before the application of the polyurethane foam interface of VAC devices leads to superior healing in db/db mice and represents a promising wound healing adjunct that can also reduce the risk of bleeding complications.

Authors+Show Affiliations

Division of Plastic and Reconstructive Surgery, University Hospitals of Basel and Lausanne, Switzerland. erbapaolo@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21814117

Citation

Erba, Paolo, et al. "Poly-N-acetyl Glucosamine Fibers Are Synergistic With Vacuum-assisted Closure in Augmenting the Healing Response of Diabetic Mice." The Journal of Trauma, vol. 71, no. 2 Suppl 1, 2011, pp. S187-93.
Erba P, Adini A, Demcheva M, et al. Poly-N-acetyl glucosamine fibers are synergistic with vacuum-assisted closure in augmenting the healing response of diabetic mice. J Trauma. 2011;71(2 Suppl 1):S187-93.
Erba, P., Adini, A., Demcheva, M., Valeri, C. R., & Orgill, D. P. (2011). Poly-N-acetyl glucosamine fibers are synergistic with vacuum-assisted closure in augmenting the healing response of diabetic mice. The Journal of Trauma, 71(2 Suppl 1), pp. S187-93. doi:10.1097/TA.0b013e318225583c.
Erba P, et al. Poly-N-acetyl Glucosamine Fibers Are Synergistic With Vacuum-assisted Closure in Augmenting the Healing Response of Diabetic Mice. J Trauma. 2011;71(2 Suppl 1):S187-93. PubMed PMID: 21814117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Poly-N-acetyl glucosamine fibers are synergistic with vacuum-assisted closure in augmenting the healing response of diabetic mice. AU - Erba,Paolo, AU - Adini,Avner, AU - Demcheva,Marina, AU - Valeri,C Robert, AU - Orgill,Dennis P, PY - 2011/8/5/entrez PY - 2011/8/10/pubmed PY - 2011/10/25/medline SP - S187 EP - 93 JF - The Journal of trauma JO - J Trauma VL - 71 IS - 2 Suppl 1 N2 - BACKGROUND: Vacuum-assisted closure (VAC) has become the preferred modality to treat many complex wounds but could be further improved by methods that minimize bleeding and facilitate wound epithelialization. Short fiber poly-N-acetyl glucosamine nanofibers (sNAG) are effective hemostatic agents that activate platelets and facilitate wound epithelialization. We hypothesized that sNAG used in combination with the VAC device could be synergistic in promoting wound healing while minimizing the risk of bleeding. METHODS: Membranes consisting entirely of sNAG nanofibers were applied immediately to dorsal excisional wounds of db/db mice followed by application of the VAC device. Wound healing kinetics, angiogenesis, and wound-related growth factor expression were measured. RESULTS: The application of sNAG membranes to wounds 24 hours before application of the VAC device was associated with a significant activation of wounds (expression of PDGF, TGFβ, EGF), superior granulation tissue formation rich in Collagen I as well as superior wound epithelialization (8.6% ± 0.3% vs. 1.8% ± 1.1% of initial wound size) and wound contraction. CONCLUSIONS: The application of sNAG fiber-containing membranes before the application of the polyurethane foam interface of VAC devices leads to superior healing in db/db mice and represents a promising wound healing adjunct that can also reduce the risk of bleeding complications. SN - 1529-8809 UR - https://www.unboundmedicine.com/medline/citation/21814117/Poly_N_acetyl_glucosamine_fibers_are_synergistic_with_vacuum_assisted_closure_in_augmenting_the_healing_response_of_diabetic_mice_ L2 - http://Insights.ovid.com/pubmed?pmid=21814117 DB - PRIME DP - Unbound Medicine ER -