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Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescens.
Appl Microbiol Biotechnol. 2012 Feb; 93(3):1231-9.AM

Abstract

Through bioassay-guided fractionation, the EtOAc extract of a culture broth of the endophytic fungus Phoma species ZJWCF006 in Arisaema erubescens afforded a new α-tetralone derivative, (3S)-3,6,7-trihydroxy-α-tetralone (1), together with cercosporamide (2), β-sitosterol (3), and trichodermin (4). The structures of compounds were established on the basis of spectroscopic analyses. Compounds 1, 2, and 3 were obtained from Phoma species for the first time. Additionally, the compounds were subjected to bioactivity assays, including antimicrobial activity, against four plant pathogenic fungi (Fusarium oxysporium, Rhizoctonia solani, Colletotrichum gloeosporioides, and Magnaporthe oryzae) and two plant pathogenic bacteria (Xanthomonas campestris and Xanthomonas oryzae), as well as in vitro antitumor activities against HT-29, SMMC-772, MCF-7, HL-60, MGC80-3, and P388 cell lines. Compound 1 showed growth inhibition against F. oxysporium and R. solani with EC₅₀ values of 413.22 and 48.5 μg/mL, respectively. Additionally, compound 1 showed no cytotoxicity, whereas compound 2 exhibited cytotoxic activity against the six tumor cell lines tested, with IC₅₀ values of 9.3 ± 2.8, 27.87 ± 1.78, 48.79 ± 2.56, 37.57 ± 1.65, 27.83 ± 0.48, and 30.37 ± 0.28 μM, respectively. We conclude that endophytic Phoma are promising sources of natural bioactive and novel metabolites.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang LW
Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Xu BG
No affiliation info available
Wang JY
No affiliation info available
Su ZZ
No affiliation info available
Lin FC
No affiliation info available
Zhang CL
No affiliation info available
Kubicek CP
No affiliation info available

MeSH

Anti-Bacterial AgentsAntifungal AgentsAntineoplastic AgentsArisaemaAscomycotaBenzofuransCell Line, TumorCulture Media, ConditionedEndophytesFungiHL-60 CellsHT29 CellsHumansMedicine, Chinese TraditionalPlant DiseasesSitosterolsSpecies SpecificityTetralonesTrichoderminXanthomonas

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21814808

Citation

Wang, Li-Wei, et al. "Bioactive Metabolites From Phoma Species, an Endophytic Fungus From the Chinese Medicinal Plant Arisaema Erubescens." Applied Microbiology and Biotechnology, vol. 93, no. 3, 2012, pp. 1231-9.
Wang LW, Xu BG, Wang JY, et al. Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescens. Appl Microbiol Biotechnol. 2012;93(3):1231-9.
Wang, L. W., Xu, B. G., Wang, J. Y., Su, Z. Z., Lin, F. C., Zhang, C. L., & Kubicek, C. P. (2012). Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescens. Applied Microbiology and Biotechnology, 93(3), 1231-9. https://doi.org/10.1007/s00253-011-3472-3
Wang LW, et al. Bioactive Metabolites From Phoma Species, an Endophytic Fungus From the Chinese Medicinal Plant Arisaema Erubescens. Appl Microbiol Biotechnol. 2012;93(3):1231-9. PubMed PMID: 21814808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescens. AU - Wang,Li-Wei, AU - Xu,Bai-Ge, AU - Wang,Jia-Ying, AU - Su,Zhen-Zhu, AU - Lin,Fu-Cheng, AU - Zhang,Chu-Long, AU - Kubicek,Christian P, Y1 - 2011/08/04/ PY - 2011/02/25/received PY - 2011/07/10/accepted PY - 2011/06/25/revised PY - 2011/8/5/entrez PY - 2011/8/5/pubmed PY - 2012/4/18/medline SP - 1231 EP - 9 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 93 IS - 3 N2 - Through bioassay-guided fractionation, the EtOAc extract of a culture broth of the endophytic fungus Phoma species ZJWCF006 in Arisaema erubescens afforded a new α-tetralone derivative, (3S)-3,6,7-trihydroxy-α-tetralone (1), together with cercosporamide (2), β-sitosterol (3), and trichodermin (4). The structures of compounds were established on the basis of spectroscopic analyses. Compounds 1, 2, and 3 were obtained from Phoma species for the first time. Additionally, the compounds were subjected to bioactivity assays, including antimicrobial activity, against four plant pathogenic fungi (Fusarium oxysporium, Rhizoctonia solani, Colletotrichum gloeosporioides, and Magnaporthe oryzae) and two plant pathogenic bacteria (Xanthomonas campestris and Xanthomonas oryzae), as well as in vitro antitumor activities against HT-29, SMMC-772, MCF-7, HL-60, MGC80-3, and P388 cell lines. Compound 1 showed growth inhibition against F. oxysporium and R. solani with EC₅₀ values of 413.22 and 48.5 μg/mL, respectively. Additionally, compound 1 showed no cytotoxicity, whereas compound 2 exhibited cytotoxic activity against the six tumor cell lines tested, with IC₅₀ values of 9.3 ± 2.8, 27.87 ± 1.78, 48.79 ± 2.56, 37.57 ± 1.65, 27.83 ± 0.48, and 30.37 ± 0.28 μM, respectively. We conclude that endophytic Phoma are promising sources of natural bioactive and novel metabolites. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/21814808/Bioactive_metabolites_from_Phoma_species_an_endophytic_fungus_from_the_Chinese_medicinal_plant_Arisaema_erubescens_ L2 - https://dx.doi.org/10.1007/s00253-011-3472-3 DB - PRIME DP - Unbound Medicine ER -