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[Identification of foliumn pyrrosiae from different habitats and species by HPLC fingerprint].
Zhong Yao Cai. 2011 Jan; 34(1):20-6.ZY

Abstract

OBJECTIVE

To establish HPLC fingerprint of Folium Pyrrosiae for identification.

METHODS

The HPLC method was developed with Diamonsil C18 column (250 mm x 4.6 mm, 5 microm),and a mixture liquid of acetonitrile-0.8% acetic acid solution as mobile phase in a gradient elution. HPLC fingerprints of44 samples were analyzed by similarity, cluster and principal component analysis.

RESULTS

The HPLC fingerprint common pattern of Pyrrosia petiolosa, Pyrrosia lingua and common pattern of Pyrrosia sheareri were set up separately. Samples from different species were classified based on the result of cluster and principal component analysis. Fingerprints of Pyrrosia sheareri and Pyrrosia lingua have high degree of similarity, but were different from Pyrrosia petiolosa, while Pyrrosia calvata and Pyrrosia assimlis were classified as adulterants with their dissimilar fingerprints.

CONCLUSION

The method is stable and reliable with a good reproducibility and provides a reference standard for identifying Folium Pyrrosiae from different habitats and species.

Authors+Show Affiliations

Zhang YX
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Li KT
No affiliation info available
Li QY
No affiliation info available
Shi Y
No affiliation info available

MeSH

Anti-Inflammatory Agents, Non-SteroidalChlorogenic AcidChromatography, High Pressure LiquidCluster AnalysisDrugs, Chinese HerbalPlant Components, AerialPlants, MedicinalPolypodiaceaeQuality ControlReproducibility of ResultsSaponins

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

21818964

Citation

Zhang, Yi-xuan, et al. "[Identification of Foliumn Pyrrosiae From Different Habitats and Species By HPLC Fingerprint]." Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, vol. 34, no. 1, 2011, pp. 20-6.
Zhang YX, Li KT, Li QY, et al. [Identification of foliumn pyrrosiae from different habitats and species by HPLC fingerprint]. Zhong Yao Cai. 2011;34(1):20-6.
Zhang, Y. X., Li, K. T., Li, Q. Y., & Shi, Y. (2011). [Identification of foliumn pyrrosiae from different habitats and species by HPLC fingerprint]. Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials, 34(1), 20-6.
Zhang YX, et al. [Identification of Foliumn Pyrrosiae From Different Habitats and Species By HPLC Fingerprint]. Zhong Yao Cai. 2011;34(1):20-6. PubMed PMID: 21818964.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Identification of foliumn pyrrosiae from different habitats and species by HPLC fingerprint]. AU - Zhang,Yi-xuan, AU - Li,Kai-tong, AU - Li,Qiu-yue, AU - Shi,Yue, PY - 2011/8/9/entrez PY - 2011/8/9/pubmed PY - 2012/1/6/medline SP - 20 EP - 6 JF - Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials JO - Zhong Yao Cai VL - 34 IS - 1 N2 - OBJECTIVE: To establish HPLC fingerprint of Folium Pyrrosiae for identification. METHODS: The HPLC method was developed with Diamonsil C18 column (250 mm x 4.6 mm, 5 microm),and a mixture liquid of acetonitrile-0.8% acetic acid solution as mobile phase in a gradient elution. HPLC fingerprints of44 samples were analyzed by similarity, cluster and principal component analysis. RESULTS: The HPLC fingerprint common pattern of Pyrrosia petiolosa, Pyrrosia lingua and common pattern of Pyrrosia sheareri were set up separately. Samples from different species were classified based on the result of cluster and principal component analysis. Fingerprints of Pyrrosia sheareri and Pyrrosia lingua have high degree of similarity, but were different from Pyrrosia petiolosa, while Pyrrosia calvata and Pyrrosia assimlis were classified as adulterants with their dissimilar fingerprints. CONCLUSION: The method is stable and reliable with a good reproducibility and provides a reference standard for identifying Folium Pyrrosiae from different habitats and species. SN - 1001-4454 UR - https://www.unboundmedicine.com/medline/citation/21818964/[Identification_of_foliumn_pyrrosiae_from_different_habitats_and_species_by_HPLC_fingerprint]_ DB - PRIME DP - Unbound Medicine ER -