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Sorbitol-fermenting, β-glucuronidase-positive, Shiga toxin-negative Escherichia coli O157:H7 in free-ranging red deer in South-Central Spain.
Foodborne Pathog Dis. 2011 Dec; 8(12):1313-5.FP

Abstract

We investigated the prevalence of Escherichia coli O157:H7 in free-ranging red deer in south-central Spain, to assess their potential as reservoir hosts of sorbitol-fermenting (SF) E. coli O157:H7 strains, which are emerging causes of hemolytic uremic syndrome in Europe. Fecal samples from 264 hunter-harvested Iberian red deer (Cervus elaphus) were collected in 25 different game estates and examined for E. coli O157:H7 by culture and PCR. E. coli O157:H7 was detected and isolated in 4 of the 25 game estates sampled (16%) and the isolates obtained (four in total) were further phenogenotypically characterized. One of them was biochemically typical of E. coli O157:H7, that is, neither fermented sorbitol nor exhibited β-glucuronidase (GUD) activity, and carried genes encoding Shiga toxins (Stx) 1 and 2, the intimin subtype γ1, the enterohemorrhagic E. coli (EHEC)-hemolysin, and the ter gene cluster. The rest of the isolates (three of four) fermented sorbitol, exhibited GUD activity after 18-24 h incubation, and carried genes encoding the intimin subtype γ1 and the EHEC-hemolysin, although no Stx-encoding genes were detected. All these atypical isolates carried the sfp gene cluster, lacked the ter gene cluster, and were unable to grow on cefixime tellurite sorbitol MacConkey agar, which are typical features of SF E. coli O157:H7 strains isolated from patients. In total, SF, GUD-positive, Stx-negative E. coli O157:H7 strains were isolated in 3 of the 25 game estates sampled (12%), with an overall sample-level prevalence of 1.1% (3/264). Our findings indicate that free-ranging red deer may be one of the possible reservoir hosts of Stx-negative derivatives of SF E. coli O157:H7.

Authors+Show Affiliations

Instituto de Investigación en Recursos Cinegéticos, CSIC-UCLM-JCCM, Ciudad Real, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21819212

Citation

Díaz, Sandra, et al. "Sorbitol-fermenting, Β-glucuronidase-positive, Shiga Toxin-negative Escherichia Coli O157:H7 in Free-ranging Red Deer in South-Central Spain." Foodborne Pathogens and Disease, vol. 8, no. 12, 2011, pp. 1313-5.
Díaz S, Vidal D, Herrera-León S, et al. Sorbitol-fermenting, β-glucuronidase-positive, Shiga toxin-negative Escherichia coli O157:H7 in free-ranging red deer in South-Central Spain. Foodborne Pathog Dis. 2011;8(12):1313-5.
Díaz, S., Vidal, D., Herrera-León, S., & Sánchez, S. (2011). Sorbitol-fermenting, β-glucuronidase-positive, Shiga toxin-negative Escherichia coli O157:H7 in free-ranging red deer in South-Central Spain. Foodborne Pathogens and Disease, 8(12), 1313-5. https://doi.org/10.1089/fpd.2011.0923
Díaz S, et al. Sorbitol-fermenting, Β-glucuronidase-positive, Shiga Toxin-negative Escherichia Coli O157:H7 in Free-ranging Red Deer in South-Central Spain. Foodborne Pathog Dis. 2011;8(12):1313-5. PubMed PMID: 21819212.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sorbitol-fermenting, β-glucuronidase-positive, Shiga toxin-negative Escherichia coli O157:H7 in free-ranging red deer in South-Central Spain. AU - Díaz,Sandra, AU - Vidal,Dolors, AU - Herrera-León,Silvia, AU - Sánchez,Sergio, Y1 - 2011/08/05/ PY - 2011/8/9/entrez PY - 2011/8/9/pubmed PY - 2013/6/20/medline SP - 1313 EP - 5 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 8 IS - 12 N2 - We investigated the prevalence of Escherichia coli O157:H7 in free-ranging red deer in south-central Spain, to assess their potential as reservoir hosts of sorbitol-fermenting (SF) E. coli O157:H7 strains, which are emerging causes of hemolytic uremic syndrome in Europe. Fecal samples from 264 hunter-harvested Iberian red deer (Cervus elaphus) were collected in 25 different game estates and examined for E. coli O157:H7 by culture and PCR. E. coli O157:H7 was detected and isolated in 4 of the 25 game estates sampled (16%) and the isolates obtained (four in total) were further phenogenotypically characterized. One of them was biochemically typical of E. coli O157:H7, that is, neither fermented sorbitol nor exhibited β-glucuronidase (GUD) activity, and carried genes encoding Shiga toxins (Stx) 1 and 2, the intimin subtype γ1, the enterohemorrhagic E. coli (EHEC)-hemolysin, and the ter gene cluster. The rest of the isolates (three of four) fermented sorbitol, exhibited GUD activity after 18-24 h incubation, and carried genes encoding the intimin subtype γ1 and the EHEC-hemolysin, although no Stx-encoding genes were detected. All these atypical isolates carried the sfp gene cluster, lacked the ter gene cluster, and were unable to grow on cefixime tellurite sorbitol MacConkey agar, which are typical features of SF E. coli O157:H7 strains isolated from patients. In total, SF, GUD-positive, Stx-negative E. coli O157:H7 strains were isolated in 3 of the 25 game estates sampled (12%), with an overall sample-level prevalence of 1.1% (3/264). Our findings indicate that free-ranging red deer may be one of the possible reservoir hosts of Stx-negative derivatives of SF E. coli O157:H7. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/21819212/Sorbitol_fermenting_β_glucuronidase_positive_Shiga_toxin_negative_Escherichia_coli_O157:H7_in_free_ranging_red_deer_in_South_Central_Spain_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2011.0923?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -