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Antitumor efficacy and amelioration of oxidative stress by Trichosanthes dioica root against Ehrlich ascites carcinoma in mice.

Abstract

CONTEXT

Trichosanthes dioica Roxb. (Cucurbitaceae) is a dioecious climber, traditionally used in India for several medicinal purposes.

OBJECTIVE

The present study assessed the hydroalcoholic extract of T. dioica root (TDA) for antitumor effect and antioxidant influence against Ehrlich ascites carcinoma (EAC) in Swiss albino mice.

METHODS

Twenty four hours after intraperitoneal inoculation of tumor (EAC) cells in mice, TDA was administered at 5 and 10 mg/kg body weight daily for 9 consecutive days. On the 10th day, half of the mice were sacrificed for estimation of tumor proliferation, hematological, and liver antioxidant parameters viz. lipid peroxidation, reduced glutathione (GSH), glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT); and the rest were kept alive for assessment of increase in life span. The antitumor effect of TDA was assessed by evaluating tumor weight, tumor volume, packed cell volume, viable and non-viable tumor cell counts, median survival time and percentage increase in life span of EAC bearing mice.

RESULTS AND DISCUSSION

TDA exhibited dose dependent and significant (p < 0.001) decrease in tumor weight, tumor volume, packed cell volume and viable cell count and extended the life span of EAC bearing hosts. Hematological profiles were significantly (p < 0.001) restored near to normal in TDA treated mice as compared to EAC control. TDA treatment significantly (p < 0.001) modulated the aforesaid liver antioxidant parameters as compared to EAC control.

CONCLUSION

The present study demonstrated that TDA possessed promising antitumor efficacy in mice, plausibly mediated by amelioration of oxidative stress by multiple mechanisms.

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  • Authors+Show Affiliations

    ,

    Bengal School of Technology (A College of Pharmacy), Sugandha, Hooghly, West Bengal, India.

    , , ,

    Source

    Pharmaceutical biology 49:9 2011 Sep pg 927-35

    MeSH

    Animals
    Antineoplastic Agents
    Antioxidants
    Ascites
    Body Weight
    Carcinoma, Ehrlich Tumor
    Cell Count
    Cell Survival
    Drug Administration Schedule
    Glutathione
    Hematologic Tests
    India
    Male
    Malondialdehyde
    Mice
    Oxidative Stress
    Phytotherapy
    Plant Extracts
    Plant Roots
    Plants, Medicinal
    Superoxide Dismutase
    Trichosanthes
    Tumor Burden

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    21819262