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Identification of CD3ε, CD4, CD8β splice variants of Atlantic salmon.
Fish Shellfish Immunol 2011; 31(6):815-22FS

Abstract

In vertebrates, CD3 complex and CD4 and CD8 co-receptors are essential for signal transduction during T cell activation. In the present study, we report the mRNA spliced variants of the Atlantic salmon CD3ε, CD4 and CD8β and the effect of pathogen encounter on the expression of these variants. CD3ε is alternatively spliced in thymus, head kidney, spleen and gills to give rise to the complete mRNA sequence and to an alternative product that lacks the transmembrane exon. CD4 is also alternatively spliced in the thymus, head kidney, spleen and gills to form two variants, although the alternative product is barely detectable. The alternative product lacks the exon 1B encoding the D1 domain, which is essential for binding to MHC class II proteins. Two amplicons were also found for the CD8β gene; sequencing analysis revealed that the main PCR product corresponds to the previously reported CD8β sequence, whereas the variant sequence encodes a potential protein that lacks the Ig-like domain. The expression of CD3, CD4, CD8β genes also analyzed in head kidney of LPS-treated and IPNV infected salmon and different patterns of expression were observed. The presence and balance of the different variants of T cell co-receptors could be related to the ability of fish to induce a particular type of immune response, as well as, the ability of the pathogen to modify the fish immune response.

Authors+Show Affiliations

Laboratorio de Inmunología, Centro de Biotecnología Acuícola (CBA), Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Alameda 3363, Correo 40, Casilla 33, Santiago, Chile.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21821134

Citation

Maisey, Kevin, et al. "Identification of CD3ε, CD4, CD8β Splice Variants of Atlantic Salmon." Fish & Shellfish Immunology, vol. 31, no. 6, 2011, pp. 815-22.
Maisey K, Toro-Ascuy D, Montero R, et al. Identification of CD3ε, CD4, CD8β splice variants of Atlantic salmon. Fish Shellfish Immunol. 2011;31(6):815-22.
Maisey, K., Toro-Ascuy, D., Montero, R., Reyes-López, F. E., & Imarai, M. (2011). Identification of CD3ε, CD4, CD8β splice variants of Atlantic salmon. Fish & Shellfish Immunology, 31(6), pp. 815-22. doi:10.1016/j.fsi.2011.07.022.
Maisey K, et al. Identification of CD3ε, CD4, CD8β Splice Variants of Atlantic Salmon. Fish Shellfish Immunol. 2011;31(6):815-22. PubMed PMID: 21821134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of CD3ε, CD4, CD8β splice variants of Atlantic salmon. AU - Maisey,Kevin, AU - Toro-Ascuy,Daniela, AU - Montero,Ruth, AU - Reyes-López,Felipe E, AU - Imarai,Mónica, Y1 - 2011/07/30/ PY - 2010/12/27/received PY - 2011/05/12/revised PY - 2011/07/20/accepted PY - 2011/8/9/entrez PY - 2011/8/9/pubmed PY - 2012/3/14/medline SP - 815 EP - 22 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 31 IS - 6 N2 - In vertebrates, CD3 complex and CD4 and CD8 co-receptors are essential for signal transduction during T cell activation. In the present study, we report the mRNA spliced variants of the Atlantic salmon CD3ε, CD4 and CD8β and the effect of pathogen encounter on the expression of these variants. CD3ε is alternatively spliced in thymus, head kidney, spleen and gills to give rise to the complete mRNA sequence and to an alternative product that lacks the transmembrane exon. CD4 is also alternatively spliced in the thymus, head kidney, spleen and gills to form two variants, although the alternative product is barely detectable. The alternative product lacks the exon 1B encoding the D1 domain, which is essential for binding to MHC class II proteins. Two amplicons were also found for the CD8β gene; sequencing analysis revealed that the main PCR product corresponds to the previously reported CD8β sequence, whereas the variant sequence encodes a potential protein that lacks the Ig-like domain. The expression of CD3, CD4, CD8β genes also analyzed in head kidney of LPS-treated and IPNV infected salmon and different patterns of expression were observed. The presence and balance of the different variants of T cell co-receptors could be related to the ability of fish to induce a particular type of immune response, as well as, the ability of the pathogen to modify the fish immune response. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/21821134/Identification_of_CD3ε_CD4_CD8β_splice_variants_of_Atlantic_salmon_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(11)00287-7 DB - PRIME DP - Unbound Medicine ER -