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Neuropeptide Y stimulates proliferation and migration in the 4T1 breast cancer cell line.
Int J Cancer. 2012 Jul 15; 131(2):276-86.IJ

Abstract

Stress has long been thought of to be associated with increased risk of cancer. Chronic stress is associated with elevated levels of sympathetic neurotransmitter (norepinephrine and neuropeptide Y: NPY) release and immunosuppression. The expression of NPY receptors has been reported in human breast carcinomas. Recently, activation of the NPY Y5 receptor was shown to stimulate cell growth and increase migration in human breast cancer cells; however the effects of NPY have yet to be investigated in a murine model of breast cancer. Thus, the specific aims of the current study were to: (i) characterize NPY receptor expression in 4T1 breast cancer cells and orthotopic tumors grown in BALB/c mice and (ii) investigate the impact of NPY receptor activation on 4T1 cell proliferation and migration in vitro. Positive expression of NPY receptors (Y1R, Y2R and Y5R) was observed in cells and tumor tissue. As well, NPY treatment of 4T1 cells promoted a concentration-dependent increase in proliferation, through increased phosphorylation of ERK 1/2. Using NPY receptor antagonists (Y1R:BIBP3226, Y2R:BIIE0246 and Y5R:L-152,804), we found the proliferative response to be Y5R mediated. Additionally, NPY increased chemotaxis through Y2R and Y5R activation. These data are in congruence with those from human cell lines and highlight the 4T1 cell line as a translatable model of breast cancer in which the effects of NPY can be studied in an immunocompetent system.

Authors+Show Affiliations

Department of Medical Biophysics, The University of Western Ontario, London, Ontario, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21823118

Citation

Medeiros, Philip J., et al. "Neuropeptide Y Stimulates Proliferation and Migration in the 4T1 Breast Cancer Cell Line." International Journal of Cancer, vol. 131, no. 2, 2012, pp. 276-86.
Medeiros PJ, Al-Khazraji BK, Novielli NM, et al. Neuropeptide Y stimulates proliferation and migration in the 4T1 breast cancer cell line. Int J Cancer. 2012;131(2):276-86.
Medeiros, P. J., Al-Khazraji, B. K., Novielli, N. M., Postovit, L. M., Chambers, A. F., & Jackson, D. N. (2012). Neuropeptide Y stimulates proliferation and migration in the 4T1 breast cancer cell line. International Journal of Cancer, 131(2), 276-86. https://doi.org/10.1002/ijc.26350
Medeiros PJ, et al. Neuropeptide Y Stimulates Proliferation and Migration in the 4T1 Breast Cancer Cell Line. Int J Cancer. 2012 Jul 15;131(2):276-86. PubMed PMID: 21823118.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuropeptide Y stimulates proliferation and migration in the 4T1 breast cancer cell line. AU - Medeiros,Philip J, AU - Al-Khazraji,Baraa K, AU - Novielli,Nicole M, AU - Postovit,Lynne M, AU - Chambers,Ann F, AU - Jackson,Dwayne N, Y1 - 2011/09/16/ PY - 2010/11/18/received PY - 2011/07/26/accepted PY - 2011/8/9/entrez PY - 2011/8/9/pubmed PY - 2012/7/31/medline SP - 276 EP - 86 JF - International journal of cancer JO - Int. J. Cancer VL - 131 IS - 2 N2 - Stress has long been thought of to be associated with increased risk of cancer. Chronic stress is associated with elevated levels of sympathetic neurotransmitter (norepinephrine and neuropeptide Y: NPY) release and immunosuppression. The expression of NPY receptors has been reported in human breast carcinomas. Recently, activation of the NPY Y5 receptor was shown to stimulate cell growth and increase migration in human breast cancer cells; however the effects of NPY have yet to be investigated in a murine model of breast cancer. Thus, the specific aims of the current study were to: (i) characterize NPY receptor expression in 4T1 breast cancer cells and orthotopic tumors grown in BALB/c mice and (ii) investigate the impact of NPY receptor activation on 4T1 cell proliferation and migration in vitro. Positive expression of NPY receptors (Y1R, Y2R and Y5R) was observed in cells and tumor tissue. As well, NPY treatment of 4T1 cells promoted a concentration-dependent increase in proliferation, through increased phosphorylation of ERK 1/2. Using NPY receptor antagonists (Y1R:BIBP3226, Y2R:BIIE0246 and Y5R:L-152,804), we found the proliferative response to be Y5R mediated. Additionally, NPY increased chemotaxis through Y2R and Y5R activation. These data are in congruence with those from human cell lines and highlight the 4T1 cell line as a translatable model of breast cancer in which the effects of NPY can be studied in an immunocompetent system. SN - 1097-0215 UR - https://www.unboundmedicine.com/medline/citation/21823118/Neuropeptide_Y_stimulates_proliferation_and_migration_in_the_4T1_breast_cancer_cell_line_ L2 - https://doi.org/10.1002/ijc.26350 DB - PRIME DP - Unbound Medicine ER -