Tags

Type your tag names separated by a space and hit enter

Characterization and identification of multiple constituents in Yinhuang granules by high-performance liquid chromatography with diode-array and time-of-flight mass spectrometry detection.
J Sep Sci. 2011 Oct; 34(19):2566-75.JS

Abstract

A fast high-performance liquid chromatography (HPLC) method with diode-array detection (DAD) and time-of-flight mass spectrometry (TOF/MS) has been developed for the analysis of multi-constituent in Yinhuang granules, a well-known combined herbal remedy prepared from the extract mixtures of Flos Lonicerae and Radix Scutellariae. The fast HPLC analysis was performed on an Agilent ZorBax SB-C(18) column (4.6×50 mm, 1.8 μm) and 0.2% aqueous formic acid and acetonitrile was the optimum mobile phase for gradient elution in 17 min, which is five times faster than the performance of conventional columns packed with 5.0 μm particles. With various fragmentor voltages in TOF/MS, accurate mass measurements (<5 ppm error) for molecular ions and characteristic fragment ions represented reliable identification criteria for different constituents. A total of 28 compounds, including nine phenolic acids, three iridoid glycosides and nine saponins from Flos Lonicerae and seven flavonoids from Radix Scutellariae, were identified or tentatively characterized in the extract of Yinhuang granules. The established fast HPLC-DAD-TOF/MS method turns out to be useful and efficient for quality control of this commonly used Chinese herbal preparation.

Authors+Show Affiliations

Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Ministry of Education, Nanjing, P. R. China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21823224

Citation

Liu, E-Hu, et al. "Characterization and Identification of Multiple Constituents in Yinhuang Granules By High-performance Liquid Chromatography With Diode-array and Time-of-flight Mass Spectrometry Detection." Journal of Separation Science, vol. 34, no. 19, 2011, pp. 2566-75.
Liu EH, Liu Q, Chu C, et al. Characterization and identification of multiple constituents in Yinhuang granules by high-performance liquid chromatography with diode-array and time-of-flight mass spectrometry detection. J Sep Sci. 2011;34(19):2566-75.
Liu, E. H., Liu, Q., Chu, C., & Li, P. (2011). Characterization and identification of multiple constituents in Yinhuang granules by high-performance liquid chromatography with diode-array and time-of-flight mass spectrometry detection. Journal of Separation Science, 34(19), 2566-75. https://doi.org/10.1002/jssc.201100254
Liu EH, et al. Characterization and Identification of Multiple Constituents in Yinhuang Granules By High-performance Liquid Chromatography With Diode-array and Time-of-flight Mass Spectrometry Detection. J Sep Sci. 2011;34(19):2566-75. PubMed PMID: 21823224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and identification of multiple constituents in Yinhuang granules by high-performance liquid chromatography with diode-array and time-of-flight mass spectrometry detection. AU - Liu,E-Hu, AU - Liu,Qun, AU - Chu,Chu, AU - Li,Ping, Y1 - 2011/08/08/ PY - 2011/03/21/received PY - 2011/06/15/revised PY - 2011/06/22/accepted PY - 2011/8/9/entrez PY - 2011/8/9/pubmed PY - 2012/1/12/medline SP - 2566 EP - 75 JF - Journal of separation science JO - J Sep Sci VL - 34 IS - 19 N2 - A fast high-performance liquid chromatography (HPLC) method with diode-array detection (DAD) and time-of-flight mass spectrometry (TOF/MS) has been developed for the analysis of multi-constituent in Yinhuang granules, a well-known combined herbal remedy prepared from the extract mixtures of Flos Lonicerae and Radix Scutellariae. The fast HPLC analysis was performed on an Agilent ZorBax SB-C(18) column (4.6×50 mm, 1.8 μm) and 0.2% aqueous formic acid and acetonitrile was the optimum mobile phase for gradient elution in 17 min, which is five times faster than the performance of conventional columns packed with 5.0 μm particles. With various fragmentor voltages in TOF/MS, accurate mass measurements (<5 ppm error) for molecular ions and characteristic fragment ions represented reliable identification criteria for different constituents. A total of 28 compounds, including nine phenolic acids, three iridoid glycosides and nine saponins from Flos Lonicerae and seven flavonoids from Radix Scutellariae, were identified or tentatively characterized in the extract of Yinhuang granules. The established fast HPLC-DAD-TOF/MS method turns out to be useful and efficient for quality control of this commonly used Chinese herbal preparation. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/21823224/Characterization_and_identification_of_multiple_constituents_in_Yinhuang_granules_by_high_performance_liquid_chromatography_with_diode_array_and_time_of_flight_mass_spectrometry_detection_ L2 - https://doi.org/10.1002/jssc.201100254 DB - PRIME DP - Unbound Medicine ER -