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Similar potency of catechin and its enantiomers in alleviating 1-methyl-4-phenylpyridinium ion cytotoxicity in SH-SY5Y cells.
J Pharm Pharmacol. 2011 Sep; 63(9):1169-74.JP

Abstract

OBJECTIVES

Previously, the flavonoid (±)-catechin was shown to exert potent neuroprotective action in the mouse 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease model. The purpose of this study was to investigate whether the different enantiomers of catechin ((+)-catechin, (-)-catechin and (±)-catechin, a 50:50 mixture of (+)-catechin and (-)-catechin) could protect SH-SY5Y cells against 1-methyl-4-phenylpyridinium ion (MPP(+)) toxicity by decreasing the generation of oxygen free radicals. The inhibitive effect of (±)-catechin on JNK/c-Jun activation was investigated.

METHODS

The effects of (+)-catechin, (-)-catechin or (±)-catechin in protecting against MPP(+) toxicity were evaluated and compared in SH-SY5Y cells by testing the release of lactate dehydrogenase. The generation of reactive oxygen species (ROS) was measured by immunochemistry and the phosphorylation level of JNK/c-Jun was determined by Western blotting.

KEY FINDINGS

In SH-SY5Y cells, (+)-catechin, (-)-catechin or (±)-catechin reduced apoptosis induced by MPP(+) and decreased ROS generation caused by MPP(+) . Different enantiomers of catechin showed protective effects at similar potency. Moreover (±)-catechin decreased JNK/c-Jun phosphorylation which was increased by MPP(+).

CONCLUSIONS

Catechin and its two enantiomers could protect SH-SY5Y cells against MPP(+) cytotoxicity at a similar potency. Antioxidative stress and inhibition of the JNK/c-Jun signalling pathway might have been involved in the neuroprotective mechanisms of catechin against MPP(+) cytotoxicity in SH-SY5Y cells.

Authors+Show Affiliations

Guangdong Institute for Drug Control, Guangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21827489

Citation

Ruan, Hao-lan, et al. "Similar Potency of Catechin and Its Enantiomers in Alleviating 1-methyl-4-phenylpyridinium Ion Cytotoxicity in SH-SY5Y Cells." The Journal of Pharmacy and Pharmacology, vol. 63, no. 9, 2011, pp. 1169-74.
Ruan HL, Yang Y, Zhu XN, et al. Similar potency of catechin and its enantiomers in alleviating 1-methyl-4-phenylpyridinium ion cytotoxicity in SH-SY5Y cells. J Pharm Pharmacol. 2011;63(9):1169-74.
Ruan, H. L., Yang, Y., Zhu, X. N., Wang, X. L., & Chen, R. Z. (2011). Similar potency of catechin and its enantiomers in alleviating 1-methyl-4-phenylpyridinium ion cytotoxicity in SH-SY5Y cells. The Journal of Pharmacy and Pharmacology, 63(9), 1169-74. https://doi.org/10.1111/j.2042-7158.2011.01293.x
Ruan HL, et al. Similar Potency of Catechin and Its Enantiomers in Alleviating 1-methyl-4-phenylpyridinium Ion Cytotoxicity in SH-SY5Y Cells. J Pharm Pharmacol. 2011;63(9):1169-74. PubMed PMID: 21827489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Similar potency of catechin and its enantiomers in alleviating 1-methyl-4-phenylpyridinium ion cytotoxicity in SH-SY5Y cells. AU - Ruan,Hao-lan, AU - Yang,Yi, AU - Zhu,Xiao-nan, AU - Wang,Xue-lan, AU - Chen,Ru-zhu, Y1 - 2011/06/21/ PY - 2011/8/11/entrez PY - 2011/8/11/pubmed PY - 2012/3/1/medline SP - 1169 EP - 74 JF - The Journal of pharmacy and pharmacology JO - J Pharm Pharmacol VL - 63 IS - 9 N2 - OBJECTIVES: Previously, the flavonoid (±)-catechin was shown to exert potent neuroprotective action in the mouse 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease model. The purpose of this study was to investigate whether the different enantiomers of catechin ((+)-catechin, (-)-catechin and (±)-catechin, a 50:50 mixture of (+)-catechin and (-)-catechin) could protect SH-SY5Y cells against 1-methyl-4-phenylpyridinium ion (MPP(+)) toxicity by decreasing the generation of oxygen free radicals. The inhibitive effect of (±)-catechin on JNK/c-Jun activation was investigated. METHODS: The effects of (+)-catechin, (-)-catechin or (±)-catechin in protecting against MPP(+) toxicity were evaluated and compared in SH-SY5Y cells by testing the release of lactate dehydrogenase. The generation of reactive oxygen species (ROS) was measured by immunochemistry and the phosphorylation level of JNK/c-Jun was determined by Western blotting. KEY FINDINGS: In SH-SY5Y cells, (+)-catechin, (-)-catechin or (±)-catechin reduced apoptosis induced by MPP(+) and decreased ROS generation caused by MPP(+) . Different enantiomers of catechin showed protective effects at similar potency. Moreover (±)-catechin decreased JNK/c-Jun phosphorylation which was increased by MPP(+). CONCLUSIONS: Catechin and its two enantiomers could protect SH-SY5Y cells against MPP(+) cytotoxicity at a similar potency. Antioxidative stress and inhibition of the JNK/c-Jun signalling pathway might have been involved in the neuroprotective mechanisms of catechin against MPP(+) cytotoxicity in SH-SY5Y cells. SN - 2042-7158 UR - https://www.unboundmedicine.com/medline/citation/21827489/Similar_potency_of_catechin_and_its_enantiomers_in_alleviating_1_methyl_4_phenylpyridinium_ion_cytotoxicity_in_SH_SY5Y_cells_ L2 - https://doi.org/10.1111/j.2042-7158.2011.01293.x DB - PRIME DP - Unbound Medicine ER -